Lipid Production by Yarrowia lipolytica B9 Using Crude Glycerol as Carbon Source

Yıl 2021, Cilt: 5 Sayı: 2, 126 - 130, 31.12.2021

Nazlı Pınar Arslan , Özden Canlı Taşar

https://doi.org/10.31594/commagene.895886

Öz

The present study was carried out to optimize some culture conditions to increase lipid production from Yarrowia lipolytica B9 in crude glycerol-based medium. The experiments displayed that too high concentrations of ammonium sulfate, KH2PO4, and MgSO4 increased cell growth but inhibited lipid synthesis. In contrast, more synthesis of lipids was determined to be achived at high concentrations of NaCl. The optimum concentrations of glycerol, ammonium sulfate, KH2PO4, MgSO4, and NaCl for lipid synthesis were determined as 50, 3, 1.5, 1 and 5 g/L, respectively. The optimal incubation time for lipid synthesis was found to be 6 days. Lipid concentration of 2.69 g/L and lipid content of 60.1%, were reached under optimal culture conditions.

Anahtar Kelimeler

oleaginous, yeast, mineral salts, optimization

Karbon Kaynağı Olarak Ham Gliserolü Kullanarak Yarrowia lipolytica B9 ile Lipit Üretimi

Öz

Mevcut çalışma, ham gliserol bazlı besiyerinde Yarrowia lipolytica B9’dan lipit üretimini artırmak için bazı kültür koşullarını optimize etmek amacıyla gerçekleştirilmiştir. İnceleme sonucunda amonyum sülfat, KH2PO4 ve MgSO4’ün çok yüksek konsantrasyonlarının hücre büyümesini artırdığı fakat lipit sentezini inhibe ettiği gözlemlendi. Buna ek olarak, daha fazla lipit sentezinin yüksek NaCl konsantrasyonlarında başarıldığı ortaya çıktı. Lipit sentezi için gliserol, amonyum sülfat, KH2PO4, MgSO4 ve NaCl’nin optimum konsantrasyonları sırasıyla 50; 3; 1,5; 1 ve 5 gr/lt olarak belirlendi. Lipid sentezi için optimal inkübasyon süresinin 6 gün olduğu bulundu. Optimal kültür koşullarında 2.69 gr/lt lipit konsantrasyonuna ve %60.1 lipit içeriğine ulaşıldı.

Anahtar Kelimeler

Yağlı, maya, mineral tuz, optimizasyon

Kaynakça

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