İğde (Elaeagnus angustifolia L.) Yaprak Ekstraktı’nın Kök ur nematodu’na Karşı Kullanımı

Öz

Bu çalışmanın amacı, Elaeagnus angustifolia L. bitkisinin metanol ekstraktının Meloidogyne incognita'ya karşı toksikolojik potansiyelini in vitro ve kontrollü koşullar altında domates üzerinde değerlendirmektir. Çalışma dört konsantrasyon (0 (seyreltilmemiş), %25, %50 ve %100 seyreltilmiş) uygulanarak gerçekleştirilmiştir. İn vitroda dokuz ml ekstrakt içeren petrilere 20 L2 ml-1 içeren 1 ml süspansiyon eklenmiştir ve 48 saat sonra ölü bireyler sayılarak ölüm oranı kaydedilmiştir. Kontrollü koşullar altında, Özkan F1 domates çeşidinde her uygulama için tesadüf parselleri deneme deseninde 5 tekerrürlü olarak kurulmuştur. Domates fideleri saksılara şaşırtıldıktan hemen sonra 1000 ppm/saksı iğde yaprağı ekstraktı konsantrasyonlarına göre toprağa uygulanmış ve iyice karıştırılmıştır. Beş gün sonra, her saksıya 500 L2 ile nematod inokulasyonu gerçekleştirilmiştir. Elli gün sonra köklerdeki gal sayısı ve yumurta paketleri sayılmıştır. İn vitro L2 mortalitesi, iğde metanol yaprak ekstraktının konsantrasyonu seyreltildiğinde azalırken, kontrollü koşullar altında domates köklerinde ur ve yumurta paketi sayısında artış olmuştur. Seyreltilmemiş iğde yaprağı metanol ekstraktının M. incognita üzerindeki baskılayıcı etkisi diğer konsantrasyona göre daha yüksek olmuştur. Elaeagnus angustifolia ekstraktının toksik potansiyele sahip olduğu ve kök ur nematodunun kontrolünde etkili bir şekilde kullanılabileceği sonucuna varılmıştır. Elaeagnacea familyasının yaprak ekstraktının nematisit etkisi ilk kez bu çalışmada gösterilmiştir.

Anahtar Kelimeler

• iğde
• methanol
• yaprak ekstraktı
• kök ur nematodu
• biyolojik mücadele

Use of Oleaster (Elaeagnus angustifolia L.) Leaf Extract against Root knot nematode Abstract

Öz

The aim of the present study was to evaluate the toxicological potential of the methanol extract of Elaeagnus angustifolia L. plant against the Meloidogyne incognita in vitro and under controlled condition on tomato. The study were conducted by applying four concentrations (0 (undiluted), 25%, 50% and 100% diluted). In vitro, 1 ml of suspension containing 20 J2 ml-1 was added to petri dishes containing nine ml of extract, and the mortality rate was recorded after 48 hours by counting the dead individuals.Under controlled conditions, randomized plots of Özkan F1 tomato cultivar were established in a trial design with 5 replications for each application. Immediately after the tomato seedlings were transplanted into the pots, the oleaster leaf extracts of 1000 ppm/pot were applied to the soil according to the concentrations and mixed thoroughly. After five days, nematode inoculation was carried out with 500 J2 in each pot. The number of galls and egg masses were counted on roots after 50 days. In vitro L2 mortality was decreased as the concentration of the oleaster methanole leaf extract was diluted, while there was an increase in the number of galls and egg masses on tomato roots under controlled conditions. The suppressive effect of undiluted oleaster leaf methanol extract on M. incognita was higher than other concentration. It is concluded that extract of E. angustifolia has toxic potential and can be effectively used for the control of root knot nematode. The nematicidal effect of the leaf extract of the Elaeagnacea family was demonstrated for the first time in this study.

Anahtar Kelimeler

• oleaster
• methanole
• leaf extract
• root knot nematode
• biological control

Kaynakça

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