Kenevir Çeşitlerinde clpP Geninin Karakterizasyonu ve Mikrosatelit Analizi

Yıl 2025, Cilt: 13 Sayı: 2, 320 - 325, 24.12.2025

Saber Delpasand Khabbazi

https://doi.org/10.33202/comuagri.1827474

Öz

Kenevir, lifleri, tohumları ve kanabinoidleri nedeniyle endüstriyel, tıbbi ve eğlence amaçlı kullanılır. Kenevir çeşitlerinin doğru bir şekilde tanımlanmasına duyulan ihtiyaç arttıkça, moleküler belirteçler çeşitlerin ayırt edilmesi, evrimsel çalışmalar ve adli analizler için önemli araçlar haline gelmiştir. Mikrosatellitler, bitki genomunun tamamında, kloroplast DNA da dahil olmak üzere, yüksek değişkenliğe sahip ve ko-dominant DNA tekrarlarıdır. Kloroplast genleri arasında, ATP’ye bağımlı Clp proteazın önemli bir alt birimini kodlayan clpP geninin, mikrosatellit varyasyonu açısından zengin bir bölge olduğu bildirilmiştir. Bu çalışma, farklı tipleri ve coğrafi kökenleri temsil eden on beş kenevir örneği üzerinde clpP genini inceleyerek, genetik belirteç olarak kullanılabilecek mikrosatellit motiflerini tanımlamayı amaçlamıştır. Bu gen, üç ekzon ve iki introndan oluşmakta ve ekzon bölgeleri tamamen korunmuştur. Tüm dizilim farklılıkları, SNP ve Indel’ler dahil olmak üzere, intron bölgelerinde yer almaktadır. Mikrosatellit analizinde, çoğunlukla A, T ve TA motiflerinden oluşan mono- ve dinükleotid tekrarlar belirlenmiştir ve bunlar çeşitler arasında uzunluk ve dizilim açısından farklılık göstermiştir. Bazı motifler belirli genotiplere özgü olup, bu durum çeşitlerin coğrafi farklılıkları yansıtmaktadır. clpP geninde tanımlanan mikrosatellit profilleri, bu gen bölgesinin kenevir genetiği için bilgilendirici olduğunu ve clpP tabanlı belirteçlerin ıslah, filogenetik çalışmalar ve adli analizlerde kullanılma potansiyeline sahip olduğunu göstermektedir.

Anahtar Kelimeler

Kenevir , kloroplast DNA , genetik çeşitlilik , genetik belirteç , mikrosatellitler

Characterization and Microsatellite Analysis of the clpP Gene in Cannabis Cultivars

Öz

Cannabis is used for industrial, medicinal, and recreational purposes due to its fibers, seeds, and cannabinoids. As the need for identification of cannabis varieties increases, molecular markers have become important tools for distinguishing cultivars, studying evolution, and supporting forensic analyses. Microsatellites are highly variable, co-dominant DNA repeats found throughout plant genomes, including chloroplast DNA. Among chloroplast genes, clpP, which encodes a key subunit of the ATP-dependent clp protease, has been reported as a region rich in microsatellite variation. This study investigated the clpP gene in fifteen cannabis accessions representing different crop types and geographical backgrounds, with the aim of identifying microsatellite motifs useful as genetic markers. The gene consisted of three exons and two introns, with the exon regions completely conserved. All sequence differences, including SNPs and Indels, were located in the introns. Microsatellite analysis detected mono- and dinucleotide repeats mainly A, T, and TA motifs that differed in length and organization among cultivars. Some motifs are specific to certain genotypes, reflecting the geographical differences of the varieties. The microsatellite patterns identified in the clpP gene indicate that this region is informative for cannabis genetics and highlight the potential of clpP-based markers for use in breeding, phylogenetic studies, and forensic analyses.

Anahtar Kelimeler

Cannabis , chloroplast DNA , genetic diversity , genetic marker , microsatellites

Kaynakça

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