Determination of Fluorescent Pseudomonas Against FusariumWilt of Cucumber (Fusarium oxysporum f. sp. cucumerinum) in Greenhouse

Öz

Fusarium oxysporum f.sp. cucumerinum is a soil–borne pathogen and can invade the host plant in all stages of developmental period. In our study, the biological control methods based on the nutritional competence and induced resistance which inhibits the F. oxysporum f. sp. cucumerinum were studied in order to achieve best integration. Fluorescent pseudomonad isolates (161) were screened for their antagonistic activity at both in vivo and in vitro conditions simultaneously, against F. oxysporum f. sp. cucumerinum. At the end of these experiment, 20 of fluorescent pseudomonas isolates, which are chosen, was tested in the terms of siderophore effect. Chosen 20 of fluorescent pseudomonas isolates, it is detected that it’s in vitro activity is generally resistant to siderophpre effect. İn the experiments which is done with this isolates, it is seen that in the assessment with in vivo, the effect changes between 7.69% and 38.4%

Anahtar Kelimeler

• Cucumber, Fusarium wilt, Fluorescent pseudomonad, Biological control

Serada Hıyar Fusarium Solgunluğu (Fusarium oxysporum f. sp. cucumerinum)’na Karşı Floresan Pseudomonasların Etkinliğinin Belirlenmesi

Öz

Toprak kaynaklı bir etmen olan Fusarium oxysporum f. sp. cucumerinum hıyar bitkisini tüm gelişme dönemlerinde hastalandırabilmektedir. Çalışmamızda Fusarium oxysporum f. sp. cucumerinum'un önlenmesinde özellikle besin elementi rekabeti ve uyarılmış dayanıklılığa dayanan biyolojik mücadelede hastalığa karşı en iyi entegrasyon yapılmaya çalışılmıştır. 161 adet floresan pseudomonas izolatı Fusarium oxysporum f.sp. cucumerinum’a karşı aynı anda in vitro ve in vivo koşullarda antagonistik yetenekleri açısından taranmıştır. Bu denemeler sonucunda seçilen 20 adet floresan pseudomonas izolatının siderefor etki yönünden testlemeleri yapılmıştır. Seçilen 20 adet floresan pseudomonas izolatının in vitro etkinlikleri genelde siderofor etkiye dayandığı belirlenmiştir. Bu izolatlar ile in vivoda yapılan değerlendirmede ise etkinin %7,69 ile %38,4 arasında değiştiği görülmüştür

Anahtar Kelimeler

• Hıyar, Fusarium solgunluğu, Floresan pseudomonas, Biyolojik mücadele

Kaynakça

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