Influence of Seed Priming with Boric Acid on Germination and Seedling Growth of Buckwheat (Fagopyrum esculentum Moench.) Under Salt Stress

Year 2024, Volume: 12 Issue: 2, 211 - 222, 27.12.2024

Semih Açıkbaş , Gülen Özyazıcı , Mehmet Arif Özyazıcı

https://doi.org/10.33202/comuagri.1538921

Abstract

Salinity is a serious problem worldwide that negatively affects plant germination, growth and productivity. Seed priming is a technique that increases seed germination, seedling development, plant growth and yield by alleviating the effects of many abiotic stress factors, especially salt stress. Present study was carried out to investigate the effects of boron (B) priming on germination and seedling development of buckwheat (Fagopyrum esculentum Moench.) under salt stress. In this study seeds of buckwheat were primed with four different concentration levels (1, 2, 3, and 4 mM) of boric acid (H3BO3) solutions. After priming, these seeds were treated with different levels (0, 100, and 200 mM) of sodium chloride (NaCl). The effect of H3BO3 and NaCl was observe on different physiological growth parameter including germination percentage, mean germination time, germination uniformity coefficient, germination energy, germination index, seedling fresh weight, and seedling vigor index were observed. It was observed that seeds without priming exhibited reduced germination performance and seedling growth under NaCl stress. Whereas priming of seeds with H3BO3 showed improved growth in germination and seedling growth traits (except for germination percentage) as compared to non-primed seeds. According to these results, it is recommended to pretreat buckwheat seeds with 1.0 mM boric acid concentration before sowing to improve germination in areas experiencing salt stress.

Keywords

Buckwheat, boric acid, salt stress, germination percentage, seedling vigor index

Tuz Stresi Altında Karabuğday (Fagopyrum esculentum Moench.)’ın Çimlenmesi ve Fide Büyümesi Üzerine Borik Asit ile Tohum Ön Uygulamasının Etkisi

Abstract

Tuzluluk, bitkilerin çimlenmesini, büyümesini ve verimliliğini olumsuz yönde etkileyen dünya çapında ciddi bir sorundur. Tohum ön uygulaması (priming), başta tuz stresi olmak üzere birçok abiyotik stres fakörlerinin etkisini hafifleterek, tohumların çimlenmesini, fide gelişimini, bitkilerin büyümesini ve verimini artıran bir tekniktir. Bu çalışma, tuz stresi altındaki karabuğday (Fagopyrum esculentum Moench.)’ın bor (B) ile priming uygulamasının çimlenme ve fide gelişimine etkisini incelemek için yürütülmüştür. Çalışmada karabuğday tohumları, dört farklı konsantrasyon seviyesinde (1, 2, 3 ve 4 mM) borik asit (H3BO3) çözeltisi ile ön uygulamaya tabi tutulmuştur. Priming uygulamasından sonra bu tohumlar, farklı seviyelerde (0, 100 ve 200 mM) sodyum klorür (NaCl) ile muamele edilmiştir. Borik asit ve tuzluluğun çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme üniformite katsayısı, çimlenme enerjisi, çimlenme indeksi, fide yaş ağırlığı ve fide güç indeksi gibi farklı fizyolojik büyüme parametreleri üzerine etkisi gözlenmiştir. Priming uygulanmayan tohumların NaCl stresi altında daha az çimlenme performansı ve fide büyümesi gösterdiği gözlemlenmiştir. Borik asit ile ön işlem uygulanan tohumlar ön işlem uygulanmayan tohumlara kıyasla çimlenme ve fide gelişim özelliklerinde (çimlenme yüzdesi hariç) daha iyi büyüme göstermiştir. Bu sonuçlara göre, tuz stresi yaşanan alanlarda, çimlenmeyi iyileştirmeye yönelik olarak ekim öncesi karabuğday tohumlarının 1.0 mM borik asit konsantrasyonu ile ön işleme tabi tutulması önerilmektedir.

Keywords

Karabuğday, borik asit, tuz stresi, çimlenme yüzdesi, fide güç indeksi

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