Gallik Asit Katklı Polivinil Alkol Nanoliflerinin Elektro Çekim Yöntemiyle Üretimi ve Morfolojik Karakterizasyonu

Yıl 2022, , 601 - 608, 17.10.2022

Halil İbrahim İçoğlu

https://doi.org/10.21605/cukurovaumfd.1189928

Öz

Elektro çekim tekniği ile üretilen nanoliflere farklı fonksiyonel özellikler kazandırmak amacıyla, polimer çözeltilerine farklı aktif maddelerin eklenmesi yaygın olarak yapılan bir uygulamadır. Birçok bitkide bulunan ve doğal bir fenolik asit olan gallik asit (GA) sahip olduğu avantajlardan (antienflamatuvar, antioksidan vb.) dolayı nanolifler içerisinde katkı maddesi olarak kullanılabilmektedir. Çalışmada, öncelikle farklı oranlarda GA içeren polivinil alkol/deiyonize su çözeltileri hazırlanmış ve çözelti özellikleri (yüzey gerilimi, iletkenlik, viskozite) tespit edilmiştir. Daha sonra bu çözeltilerden elektro çekim yöntemiyle nanolifler üretilmiştir. Üretilen nanoliflerin morfolojisi, alan emisyonlu taramalı elektron mikroskobu (FE-SEM) kullanılarak incelenmiştir. Ayrıca bir görüntü analiz yazılımı kullanılarak ortalama nanolif çapları ve çap dağılımları ölçülmüştür. GA oranı arttıkça çözelti viskozitesinin arttığı ve elektriksel iletkenliğinin azaldığı görülmüştür. Buna karşın, GA oranının çözeltilerin yüzey gerilimleri üzerinde net bir etkisi olmamıştır. Ayrıca GA oranının artması ortalama nanolif çapının artmasına neden olmuştur.

Anahtar Kelimeler

Elektro çekim, Nanolif, Polivinil alkol, Gallik asit, Morfoloji

Production and Morphological Characterization of Gallic Acid Loaded Polyvinyl Alcohol Nanofibers via Electrospinning

Öz

Adding of various active agents to polymer solutions is a common application in order to provide different functional properties to nanofibers produced by electrospinning method. Gallic acid, which is natural phenolic acid and found in various plants, can be used as additive for nanofibers due to its advantageous characteristics (anti-inflammatory, antioxidant etc.). In the study, polyvinyl alcohol/distilled water solutions containing gallic acid with different ratios were prepared and the solutions’ characteristics (surface tension, conductivity, viscosity) were measured. Then, the nanofibers were produced from those solutions via electrospinning method. The morphology of the electrospun nanofibers were investigated by field emission scanning electron microscopy (SEM). Also, average fiber diameter and variations were calculated via an image analysis software. Increasing of viscosity and decreasing of conductivity of the solutions were seen when GA ratio was increased. However, there was no effect of GA ratio on surface tension of the solutions. Additionally, increasing of GA ratio caused to increase of average fiber diameter.

Anahtar Kelimeler

Electrospinning, Nanofiber, Polyvinyl alcohol, Gallic acid, Morphology

Kaynakça

• 1. Meng, C., Xiao, Y., Wang, P., Zhang, L., Liu, Y., Tong, L., 2011. Quantum-Dot-Doped Polymer Nanofibers for Optical Sensing. Advanced Materials, 23(33), 3770-3774.
• 2. Huang, Z.M., Zhang, Y.Z., Kotaki, M., Ramakrishna, S., 2003. A Review on Polymer Nanofibers by Electrospinning and Their Applications in Nanocomposites. Composites Science and Technology, 63(15), 2223-2253.
• 3. Rutledge, G.C., Fridrikh, S.V., 2007. Formation of Fibers by Electrospinning. Advanced Drug Delivery Reviews, 59(14), 1384-1391.
• 4. Doshi, J., Reneker, D.H., 1995. Electrospinning Process and Applications of Electrospun Fibers. Journal of Electrostatics, 35(2-3), 151-160.
• 5. Baumgarten, P.K., 1934. Electrostatic Spinning of Acrylic Microfibers. Journal of Colloid and Interface Science, 36(1), 71-79.
• 6. Formhals, A., 1934. Process and Apparatus for Preparing Artificial Threads. US Patent No. 1975504.
• 7. Zeng, J., Hou, H., Wendorff, J.H., Greiner, A., 2005. Photo-Induced Solid-State Crosslinking of Electrospun Poly (vinyl alcohol) Fibers. Macromolecular Rapid Communications, 26(19), 1557-1562.
• 8. Hong, Y., Shang, T., Li, Y., Wang, L., Wang, C., Chen, X., Jing, X., 2006. Synthesis Using Electrospinning and Stabilization of Single Layer Macroporous Films and Fibrous Networks of Poly (Vinyl Alcohol). Journal of Membrane Science, 276(1-2), 1-7.
• 9. Kepekçi, R.A., İçoğlu, H.İ., Kireçci, A., 2017. Assessment of Antioxidant Activity and Phycocyanin Release of Spirulina Loaded Poly (ε-caprolactone) Electrospun Nanofibers. The Journal of the Textile Institute, 108(10), 1840-1846.
• 10. Chuysinuan, P., Thanyacharoen, T., Techasakul, S., Ummartyotin, S., 2018. Electrospun Characteristics of Gallic Acid- Loaded Polyvinyl Alcohol Fibers: Release Characteristics and Antioxidant Properties. Journal of Science: Advanced Materials and Devices, 3(2), 175-180.
• 11. Wutticharoenmongkol, P., Hannirojram, P., Nuthong, P., 2019. Gallic Acid-Loaded Electrospun Cellulose Acetate Nanofibers as Potential Wound Dressing Materials. Polymers for Advanced Technologies, 30(4), 1135-1147.
• 12. Hu, H., Nie, L., Feng, S., Suo, J., 2013. Preparation, Characterization and in Vitro Release Study of Gallic Acid Loaded Silica Nanoparticles for Controlled Release. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 68(6), 401-405.
• 13. Aytac, Z., Kusku, S.I., Durgun, E., Uyar, T. 2016. Encapsulation of Gallic Acid/ Cyclodextrin Inclusion Complex in Electrospun Polylactic Acid Nanofibers: Release Behavior and Antioxidant Activity of Gallic Acid. Materials Science and Engineering: C, 63, 231-239.
• 14. Quiles-Carrillo, L., Montanes, N., Lagaron, J. M., Balart, R., Torres-Giner, S., 2019. Bioactive Multilayer Polylactide Films with Controlled Release Capacity of Gallic Acid Accomplished by Incorporating Electrospun Nanostructured Coatings and Interlayers. Applied Sciences, 9(3), 533.
• 15. Acevedo, F., Hermosilla, J., Sanhueza, C., Mora-Lagos, B., Fuentes, I., Rubilar, M., Concheiro, A., Alvarez-Lorenzo, C., 2018. Gallic Acid Loaded PEO-Core/Zein-Shell Nanofibers for Chemopreventive Action on Gallbladder Cancer Cells. European Journal of Pharmaceutical Sciences, 119, 49-61.
• 16. Aydogdu, A., Sumnu, G., Sahin, S., 2019. Fabrication of Gallic Acid Loaded Hydroxypropyl Methylcellulose Nanofibers by Electrospinning Technique as Active Packaging Material. Carbohydrate Polymers, 208, 241-250.
• 17. Song, Y., Huang, H., He, D., Yang, M., Wang, H., Zhang, H., Li, J., Li, Y., Wang, C., 2021. Gallic Acid/2-hydroxypropyl-β-cyclodextrin Inclusion Complexes Electrospun Nanofibrous Webs: Fast Dissolution, Improved Aqueous Solubility and Antioxidant Property of Gallic Acid. Chemical Research in Chinese Universities, 37(3), 450-455.
• 18. Neo, Y.P., Swift, S., Ray, S., Gizdavic- Nikolaidis, M., Jin, J., Perera, C.O., 2013. Evaluation of Gallic Acid Loaded Zein Sub- Micron Electrospun Fibre Mats as Novel Active Packaging Materials. Food chemistry, 141(3), 3192-3200.
• 19. Neo, Y.P., Perera, C.O., Nieuwoudt, M.K., Zujovic, Z., Jin, J., Ray, S., Gizdavic- Nikolaidis, M., 2014. Influence of Heat Curing on Structure and Physicochemical Properties of Phenolic Acid Loaded Proteinaceous Electrospun Fibers. Journal of Agricultural and Food Chemistry, 62(22), 5163-5172.
• 20. Aydogdu, A., Yildiz, E., Aydogdu, Y., Sumnu, G., Sahin, S., Ayhan, Z., 2019. Enhancing Oxidative Stability of Walnuts by Using Gallic Acid Loaded Lentil Flour Based Electrospun Nanofibers as Active Packaging Material. Food Hydrocolloids, 95, 245-255.
• 21. İçoğlu, H.İ., Ceylan, Ş., Yıldırım, B., Topalbekiroğlu, M., Kılıç, A., 2021. Production of Aligned Electrospun Polyvinyl Alcohol Nanofibers via Parallel Electrode Method. The Journal of The Textile Institute, 112(6), 936-945.
• 22. Alonso, C., Lucas, R., Barba, C., Marti, M., Rubio, L., Comelles, F., Morales, J.C., Luisa, Coderch L., Parra, J.L., 2015. Skin Delivery of Antioxidant Surfactants Based on Gallic Acid and Hydroxytyrosol. Journal of Pharmacy and Pharmacology, 67(7), 900-908.
• 23. Phachamud, T., Phiriyawirut, M., 2011. In Vitro Cytotoxicity and Degradability Tests of Gallic Acid-Loaded Cellulose Acetate Electrospun Fiber. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2, 85-98.
• 24. Neo, Y.P., Ray, S., Jin, J., Gizdavic- Nikolaidis, M., Nieuwoudt, M.K., Liu, D., Quek, S.Y., 2013. Encapsulation of Food Grade Antioxidant in Natural Biopolymer by Electrospinning Technique: A Physicochemical Study Based On Zein–Gallic Acid System. Food Chemistry, 136(2), 1013-1021.
• 25. Kirecci, A., Özkoç, Ü., İçoğlu, H.İ., 2012. Determination of Optimal Production Parameters for Polyacrylonitrile Nanofibers. Journal of Applied Polymer Science, 124(6), 4961-4968.