Doku Mühendisliği Uygulamalarında Kullanılmak üzere Aljinat ve Perikardiyal Sıvı ile Hidrojel Üretimi

Öz

Yumuşak doku hasarının tedavisinde, kontrollü ilaç salınım sistemlerinde, 3D biyoyazıcılarla doku/organ üretiminde, akıllı malzeme üretiminde ve daha pek çok doku mühendisliği çalışmalarında hidrojeller kullanılmaktadır. Doğal polimerlerden elde edilen polimerler ya da sentetik olarak üretilen polimerler hidrojel üretiminde kullanılıyor olmasına rağmen çeşitli biyouyumluluk problemleri taşıyabilmektedir. Bu çalışmada 3D biyoyazıcılarda biyomürekkep üretiminde kullanılan aljinatın biyouyumluluğunu artırmak için Pericardial fluid structure (PFS) kullanılmıştır. PFS Perikardiyal sıvıdan (PF) izole edilen bir yapı olup doğal Ekstrasellüler Matrikse (ECM) hem morfolojik hem de içerik olarak çok benzeyen kompleks bileşenlerden oluşmaktadır. Dondurarak kurutulmuş olan PFS değişen oranlarda aljinat ile karıştırılarak oluşturulan grupların karşılaştırması ve analizi için SEM görüntüleme yapılmıştır. SEM görüntüleri sonuçlarına göre PFS yüksek oranda kolajen ve elastin proteini içerdiği için kolajen-elastin fiber ağı PFS eklenen gruplarda belirgin bir şekilde gözlenmiştir. PFS eklenen aljinat hidrojellerinde doğal ECM’ye benzeyen yapı daha iyi oluşturularak malzeme biyouyumluluğunun da arttırıldığı sonucuna varılmıştır.

Anahtar Kelimeler

• Aljinat
• Pericardiyal sıvı
• Hidrojel
• 3D biyoyazıcı
• Doku mühendisliği

Production of Hydrogel with Alginate and Pericardial Fluid for use in Tissue Engineering Applications

Öz

Hydrogels are used in the treatment of soft tissue damage, controlled drug release systems, tissue/organ production with 3D bioprinters, smart material production, and many other tissue engineering studies. Although polymers obtained from natural polymers or synthetically produced polymers are used in hydrogel production, they may have various biocompatibility problems. In this study, Pericardial fluid structure (PFS) was used to increase the biocompatibility of the alginate and was used in the production of bioink for use in 3D bioprinters. PFS is a structure isolated from pericardial fluid (PF) and consists of complex components that are very similar to natural Extracellular Matrix (ECM) both morphologically and in content. According to the results of SEM images, the collagen-elastin fiber network was clearly observed in the groups with PFS added, since PFS contains high levels of collagen and elastin proteins. It was concluded that the biocompatibility of the material was also increased thanks to the structure similar to the natural ECM in the alginate hydrogels with PFS added.

Anahtar Kelimeler

• Alginate
• Pericardial fluid
• Hydrogel
• 3D bioprinter
• Tissue engineering

Kaynakça

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