Öz
Micromeria fruticosa L. Druce, also called “taşnanesi”, is a Mediterranean plant with medicinal properties. In this study, response surface methodology (RSM) was used to optimize parameter values that have an effect on the concentration of menthone, isomenthone and pulegone in the supercritical CO2 extraction of Micromeria fruticosa L. Druce. For this purpose, 3-factor and 3-level Box-Behnken design was applied and temperature, pressure and time were chosen as independent variables. The assessment of results obtained were carried out by using Design-Expert software. The mathematical relationship between the responses and independent parameters were explained by quadratic equation. For supercritical CO2 extraction, the statistically significant relationship was obtained between the experimental results and predicted results. To reach the maximum concentration of menthone, isomenthone and pulegone, the optimum experimental conditions were found to be 47.16 oC, 216.99 atm ve 22.45 min. In terms of the major components of Micromeria fruticosa L. Druce, a study on the optimization of extraction conditions using supercritical CO2 has not been found in the literature. The fact that this study has been studied for the first time is considered to be important both in terms of its contribution to the literature and in terms of contributing to the pharmacology, food and nutraceuticals industries.