Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury

Derya İşler; Ferhat Şirinyıldız; Rauf Onur Ek

doi:10.17826/cumj.1002023

TR EN

Miyoglobinürik akut böbrek hasarında Ficus carica (incir) çekirdek yağı uygulamasının GSH düzeyleri, nekroz ve kast oluşumu üzerine etkisi

Öz

Amaç: Bu çalışmada, Gliserolle oluşturulmuş Deneysel Miyoglobinürük akut böbrek hasarı hayvan modelinde, soğuk pres yöntemiyle elde edilmiş olan Ficus carica (incir) çekirdeği yağının farklı dozlarda uygulanmasının, böbrek dokusu ve serum GSH düzeyi, ayrıca nekroz ve kast oluşumu üzerindeki etkisi araştırılmıştır. Gereç ve Yöntem: Ağırlığı 460-540 gr arasında değişen, 32 adet Wistar albino erkek sıçan randomize 8’erli dört gruba ayrıldı. Sham Kontrol, MAKI, MAKI+FC3, MAKI+FC6. Grupların Üre ve kreatinin düzeyleri biyokimyasal yöntemle analiz edildi. Doku nekroz düzeyi böbrek doku kesitlerinin histolojik analizi ile belirlendi. Bulgular: MAKI grubunda üre ve kreatinin düzeyi tüm gruplara göre anlamlı olarak artış gösterirken, yüksek ve düşük doz tedavi grubunda aralarında anlamlı fark olmazsızın düşük tespit edildi. MAKI grubu doku ve serum GSH düzeyi tüm gruplara göre anlamlı olarak azaldı. MAKI+FC3 ve MAKI+FC6 gruplarında, dokuda doz farkı olmaksızın, serumda ise yalnız yüksek doz ile artış tespit edildi. Böbrek dokusu kast ve nekroz düzeyinde en yüksek skor MAKI grubunda gözlenirken, tedavi gruplarında anlamlı düzeyde iyileşme tespit edildi. Sonuç: Ficus carica (incir) çekirdeği yağı ise, fonksiyonel hasarda iyileşme ve antioksidatif kapasitede artış ile birlikte morfolojik hasarda iyileşme sağlamıştır.

Anahtar Kelimeler

Antioksidan , Ficus carica çekirdek yağı , indirgenmiş glutatyon , miyoglobinürik akut böbrek hasarı , nekroz.

Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury

Abstract

Purpose: In this study, the effect of applying different doses of Ficus carica (fig) seed oil obtained by cold pressing method on the kidney tissue and serum GSH level, as well as the formation of necrosis and cast in the experimental myoglobinuric acute kidney injury animal model created with glycerol was investigated. Materials and Methods: 32 Wistar albino male rats weighing 460-540 g were randomly divided into four groups of 8 each. Sham Control, MAKI, MAKI+FC3, MAKI+FC6. Urea and creatinine levels of the groups were analyzed by biochemical method. Tissue necrosis level was determined by histological analysis of kidney tissue sections. Results: While urea and creatinine levels increased significantly in the MAKI group compared to all groups, they were found to be lower in the high and low dose treatment groups with no significant difference between them. Tissue and serum GSH levels in the MAKI group were significantly decreased compared to all groups. In the MAKI+FC3 and MAKI+FC6 groups, an increase was detected in the tissue without dose difference, and in the serum only with high dose. The highest score in kidney tissue cast and necrosis levels were observed in the MAKI group, while significant improvements were detected in the treatment groups. Conclusion: Ficus carica(fig) seed oil, provided improvement in morphological damage with improvement in functional damage and increase in antioxidative capacity.

Keywords

Antioxidant , Ficus carica seed oil , myoglobinuric acute kidney injury , necrosis , reduced glutathione.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Derya İşler
0000-0001-8235-4803
Türkiye

Ferhat Şirinyıldız ^*
0000-0001-8800-9787
Türkiye

Rauf Onur Ek
0000-0003-3923-0156
Türkiye

Yayımlanma Tarihi

31 Mart 2022

Gönderilme Tarihi

29 Eylül 2021

Kabul Tarihi

17 Ocak 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 47 Sayı: 1

DOI

https://doi.org/10.17826/cumj.1002023

IZ

https://izlik.org/JA83GS49DR

MLA

İşler, Derya, vd. “Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury”. Cukurova Medical Journal, c. 47, sy 1, Mart 2022, ss. 152-60, doi:10.17826/cumj.1002023.

Cited By

THE EFFECTS OF FICUS CARICA SEED OIL ADMINISTRATION ON RENAL TAS, TOS, OSI, PON AND TOTAL THIOL VALUES IN RENAL ISCHEMIA REPERFUSION DAMAGE

Turkish Journal of Health Science and Life

https://doi.org/10.56150/tjhsl.1293701

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury

Miyoglobinürik akut böbrek hasarında Ficus carica (incir) çekirdek yağı uygulamasının GSH düzeyleri, nekroz ve kast oluşumu üzerine etkisi

Öz

Anahtar Kelimeler

Effect of Ficus carica (fig) seed oil administration on GSH levels, necrosis and cast formation in myoglobinuric acute kidney injury

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

THE EFFECTS OF FICUS CARICA SEED OIL ADMINISTRATION ON RENAL TAS, TOS, OSI, PON AND TOTAL THIOL VALUES IN RENAL ISCHEMIA REPERFUSION DAMAGE

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Renoprotective activity of Ficus carica seed oil

PROTECTIVE AND THERAPEUTIC EFFECTS OF OLEIC ACID IN EXPERIMENTAL MYOGLOBİNURIC ACUTE RENAL FAILURE MODEL