Ileal interposition reduces oxidative stress via oxidant-antioxidant enzymes in rats with metabolic syndrome
Abstract
Purpose: This study aims to examine the effect of ileal transposition (IT) on plasma levels of the total antioxidant status (TAS), total oxidant status (TOS), Oxidative Stress Index (OSI), Superoxide dismutase (SOD), Nicotinamide adenine dinucleotide phosphate oxidase (NOX), Catalase (CAT), Reduced Glutathione (GSH) in both rats with Metabolic syndrome (MetS) and healthy controls.
Materials and Methods: In the MetS model, newborn male Wistar albino rats were given MSG (4 g/mg) on days 0, 2, 4, 6, 8, and 10. The control group was injected only saline. In the 5th month, sham and IT animals underwent selected surgery. 2 months after surgery TOS, TAS, OSI, SOD, NOX, CAT, and GSH levels were assessed in the plasma.
Results: IT procedure significantly increased SOD and CAT levels in MetS + IT group when compared to the MetS group (SOD; MetS 1.75 ± 0.04, MetS+IT 2.1 ± 0.15, CAT; MetS 32.02 ± 1.73, MetS+IT 41.64 ± 1.18,). As expected, while GSH levels was increased in MetS+IT rats compared to MetS rats, but the difference was not significant (MetS 243.31 ± 6.36, MetS+IT 269.76 ± 9.17). The NOX activity was significantly lower in MetS+IT group than MetS and MetS+S groups (MetS 610.35 ± 26.25, MetS+IT 348.86 ± 14.12).
Conclusion: These data revealed the healing effect of IT surgery against oxidative stress associated with MetS. The available data endorses IT surgery as an effective strategy to reduce oxidative damage in rats with MetS by modulating systemic oxidant and antioxidant responses.
Keywords
Metabolic syndrome , ileal interposition , oxidative stress , monosodium glutamate
Kaynakça
- 1. Lee L, Arrington Sanders R. Metabolic Syndrome. Pediatr Rev. 2012;33:459-66.
- 2. Reaven GM. Role of insulin resistance in human disease. Diabetes 1988;37:1595–607.
- 3. Roberts CK, Sindhu KK. Oxidative stress and metabolic syndrome. Life Sci. 2001;84:705–12.
- 4. Colak E, Pap D. The role of oxıdatıve stress ın the development of obesıty and obesıty-related metabolıc dısorders. J Med Biochem. 2021;40:1–9.
- 5. Ceriello A, Motz E. Is oxidative stress the pathogenic mechanism underlying insülin resistance, diabetes, and cardiovascular disease? The common soil hypothesis revisited. Arterioscler Thromb and Vasc Biol. 2004;24:816–23.
- 6. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004;114:1752–61.
- 7. Poljsak B, Šuput D, Milisav I. Achieving the balance between ros and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev. 2013;2013:956792.
- 8. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017;2017:8416763.
- 9. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5: 9–19.
- 10. Palleja A, Kashani A, Allin KH, Nielsen T, Zhang C, Li Y, et al. Roux-en-Y gastric bypass surgery of morbidly obese patients induces swift and persistent changes of the individual gut microbiota. Genome Med. 2016;8:67.
