Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis

Gamze Taşkın Şenol; Halil Mahir Kaplan; Neslihan Boyan; Özkan Oğuz; Ergin Şingirik

doi:10.17826/cumj.1091707

TR EN

Geraniol ve vitamin C’nin dietilnitrozamin kaynaklı deneysel hepatoselüler karsinogenez üzerindeki etkisi

Öz

Amaç: Bu çalışmanın amacı dietilnitrozamin (DENA) ile FL83B hepatosit hücre hattını indükleyerek oluşturulan deneysel hepatosellüler karsinogenezis (HSK) modelinde geraniol ve vitamin C'nin koruyucu etkisini araştırmaktır. Gereç ve Yöntem: Bu amaçla çalışmamızda besiyerinde hazırlanan hücreler DENA (5 μM), geraniol (5 μM) ve vitamin C (50 μM) ile 37 °C sıcaklıkta ve %5’lik CO2‘li etüvde 48 saat inkübe edildi. Hücreler inkübe edildikten sonra gruplar standart hücre kültürü plakalarında Grup 1 (Kontrol), grup 2 (DENA Kontrol), grup 3 (DENA+Geraniol), grup 4 (DENA+Vitamin C) ve grup 5 (DENA+Geraniol+Vitamin C) olmak üzere beş gruba ayrıldı. Her deney grubundan altı plaka çalışıldı. Homojenatlar santrifüj edildikten sonra yolak mediyatörlerinden NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX-2 analizleri Elisa metodu ile yapıldı. Bulgular: Kanser hücrelerinde Bax, caspase-3, COX-2, NFkB, GADD153, AIF ve GRP78 ekspresyonu grup 1 ile kıyaslandığında artmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyonun azalmakta olduğu belirlenmiştir. Bcl-2 ekspresyonu grup 1 ile kıyaslandığında azalmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyon artmaktadır. Sonuç: Bu çalışmada, DENA ile indüklenen HCC modelinde geraniol ve vitamin C uygulanan gruplarda yolak mediyatörleri aracılığıyla önemli bir hepatoprotektif etki olduğu gözlendi.

Anahtar Kelimeler

Kanser , hücre kültürü , hepatositler , karaciğer , mediyatör , yolak

Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis

Abstract

Purpose: This study aimed to investigate the protective effect of geraniol and vitamin C on the experimental hepatocellular carcinogenesis (HCC) model by inducing FL83B hepatocyte cell lines with diethylnitrosamine (DENA). Materials and Methods: The cells prepared in the medium were incubated with DENA (5 μM), geraniol (5 μM), and vitamin C (50 μM) for 48 hours in an incubator at 37 °C and 5% CO2. Groups were designed as follows: Group 1 (Control), group 2 (DENA Control), group 3 (DENA+Geraniol), group 4 (DENA+Vitamin C), and group 5 (DENA+Geraniol+Vitamin C) on standard cell culture plates. Six plates from each experimental group were studied. After the homogenization was centrifuged, analyses of pathway mediators NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX were performed by the Elisa method. Results: The expression of Bax, caspase-3, COX-2, NFkB, GADD153, AIF, and GRP78 increased in cancer cells when compared to group 1 and decreased in other groups where antiproliferative agents were applied. Bcl-2 expression is decreased when compared to group 1, and expression is increased in other groups where antiproliferative agents are applied. Conclusion: There was a significant hepatoprotective effect in the groups administered geraniol+vitamin C on pathway mediators in a DENA-induced HCC model.

Keywords

Cancer , cell culture , hepatocytes , liver , mediators , pathway

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Gamze Taşkın Şenol ^*
0000-0001-5587-1055
Türkiye

Halil Mahir Kaplan
0000-0002-1911-7327
Türkiye

Neslihan Boyan
0000-0001-9524-9535
Türkiye

Özkan Oğuz
0000-0002-3081-1467
Türkiye

Ergin Şingirik
0000-0003-1219-6744
Türkiye

Yayımlanma Tarihi

30 Haziran 2022

Gönderilme Tarihi

23 Mart 2022

Kabul Tarihi

4 Mayıs 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 47 Sayı: 2

DOI

https://doi.org/10.17826/cumj.1091707

IZ

https://izlik.org/JA62ZR79AB

MLA

Taşkın Şenol, Gamze, vd. “Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis”. Cukurova Medical Journal, c. 47, sy 2, Haziran 2022, ss. 738-46, doi:10.17826/cumj.1091707.

Cited By

Geraniol and citral: recent developments in their anticancer credentials opening new vistas in complementary cancer therapy

Zeitschrift für Naturforschung C

https://doi.org/10.1515/znc-2023-0150

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Geraniol ve vitamin C’nin dietilnitrozamin kaynaklı deneysel hepatoselüler karsinogenez üzerindeki etkisi

Öz

Anahtar Kelimeler

Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Geraniol and citral: recent developments in their anticancer credentials opening new vistas in complementary cancer therapy