Efficacy of boric acid therapy in a pseudomonas aeruginosa-induced chronic otitis media model in rats

Abdulkadir Şahin; Ayhan Kars; Korhan Kılıç; Muhammed Sedat Sakat; Sinan Köyceğiz; Serkan Yildirim

doi:10.17826/cumj.1126208

TR EN

Sıçanlarda pseudomonas aeruginosa ile indüklenen kronik otitis media modelinde borik asit tedavisinin etkinliği

Öz

Amaç: Kronik otitis media (KOM), bakteriyel veya viral patojenlerin neden olduğu orta kulağın sık görülen enfeksiyöz hastalıklarından biridir. Bu çalışmanın amacı, sıçanlarda Pseudomonas aeruginosa (P. aeruginosa) ile indüklenen KOM modelinde topikal %4’lük ve %8’lik borik asit (BA) uygulamasını sistemik siprofloksasin ile karşılaştırarak KOM tedavisinde borik asidin etkinliğini araştırmaktır. Gereç ve Yöntem: 42 Sprague Dawley cinsi sıçan 7 eşit gruba ayrıldı. P. aeruginosa ile KOM modeli oluşturuldu. Kontrol grubu, KOM grubu, topikal %4’lük ve %8’lik BA tedavi grubu, sistemik siprofloksasin tedavi grubu ve topikal %4’lük ve %8’lik BA temas grubu arasında klinik, histopatolojik ve immünohistokimyasal karşılaştırmalar yapıldı. Bulgular: KOM modelinde %4’lük BA uygulaması ile orta derecede ödem, enflamasyon, dejenerasyon ve orta derecede tümör nekroz faktör-alfa (TNF-α) ekspresyonu tespit edildi. %8’lik BA uygulaması ile hafif ödem, inflamasyon, dejenerasyon ve hafif TNF-α ekspresyonu tespit edildi. Sonuç: %4’lük ve %8’lik BA tedavisinin önemli klinik, histopatolojik ve immünohistokimyasal iyileşme sağladığı görüldü. %8’lik BA uygulamasının daha yüksek tedavi etkinliğine sahip olduğu ve orta kulak mukozasına zararlı bir etkisinin olmadığı gösterildi.

Anahtar Kelimeler

Borik asit , kronik otitis media , pseudomonas aeruginosa , sıçan , tümör nekroz faktörü-alfa

Efficacy of boric acid therapy in a pseudomonas aeruginosa-induced chronic otitis media model in rats

Abstract

Purpose: Chronic otitis media (COM) is one of the common infectious diseases of the middle ear caused by bacterial or viral pathogens. The purpose of the present study was to investigate the efficacy of boric acid (BA) in the treatment of COM by comparing topical 4% and 8% BA applications with systemic ciprofloxacin in a Pseudomonas aeruginosa (P. aeruginosa) inoculation-induced model of COM in rats. Materials and Methods: Forty-two Sprague Dawley rats were divided into seven equal groups. The COM model was created with P. aeruginosa. Clinical, histopathological and, immunohistochemical comparisons were performed between the control, COM, topical 4% and 8% BA treatment, a systemic ciprofloxacin treatment, and topical 4% and 8% BA contact groups. Results: In the COM model, moderate edema, inflammation, degeneration and moderate tumor necrosis factor-alpha (TNF-α) expression were detected with the application of 4% BA. Mild edema, inflammation, degeneration, and mild TNF-α expression were detected with 8% BA application. Conclusion: Both 4% and 8% BA treatment provided significant clinical, histopathological and immunohistochemical improvement. The application of 8% BA was found to exhibit greater therapeutic efficacy, with no harmful effect on the middle ear mucosa.

Keywords

Boric acid , chronic otitis media , pseudomonas aeruginosa , rat , tumor necrosis factor-alpha

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Abdulkadir Şahin ^*
0000-0002-8426-792X
Türkiye

Ayhan Kars
0000-0003-4580-315X
Türkiye

Korhan Kılıç
0000-0001-6048-034X
Türkiye

Muhammed Sedat Sakat
0000-0001-9588-1725
Türkiye

Sinan Köyceğiz
0000-0002-9596-6646
Türkiye

Serkan Yildirim
0000-0003-2457-3367
Türkiye

Yayımlanma Tarihi

30 Eylül 2022

Gönderilme Tarihi

5 Haziran 2022

Kabul Tarihi

8 Ağustos 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 47 Sayı: 3

DOI

https://doi.org/10.17826/cumj.1126208

IZ

https://izlik.org/JA64ZG25GZ

MLA

Şahin, Abdulkadir, vd. “Efficacy of boric acid therapy in a pseudomonas aeruginosa-induced chronic otitis media model in rats”. Cukurova Medical Journal, c. 47, sy 3, Eylül 2022, ss. 1163-71, doi:10.17826/cumj.1126208.

Cited By

Clinical, cytological, and microbiological evaluation of a topical boric acid–alcohol solution for the treatment of canine otitis externa: a randomized, open-label controlled clinical trial

Veterinary Research Communications

https://doi.org/10.1007/s11259-025-10903-9

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Sıçanlarda pseudomonas aeruginosa ile indüklenen kronik otitis media modelinde borik asit tedavisinin etkinliği

Öz

Anahtar Kelimeler

Efficacy of boric acid therapy in a pseudomonas aeruginosa-induced chronic otitis media model in rats

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Clinical, cytological, and microbiological evaluation of a topical boric acid–alcohol solution for the treatment of canine otitis externa: a randomized, open-label controlled clinical trial