Ganoderma lucidum inhibits proliferation and promotes autophagy in breast cancer cell lines with varying hormonal sensitivity by regulating Beclin-1, LC3, and p62
Öz
Purpose: The aim of this study was to investigate the role of Ganoderma lucidum (GL) in cell proliferation and autophagy in breast cancer cell lines.
Materials and Methods: Cell lines were cultured in vitro and the appropriate dose was determined by the MTT method. In order to show the effect of GL on autophagy in MCF-7 and MDA-MB-231 breast cancer cell lines, Beclin-1, LC3 and p62 expression were examined by immunohistochemistry staining. Additionally, cell cycle analysis was carried out in GL-treated and untreated cells using a cell cycle analysis kit to look into how GL affects cell division.
Results: Viability level was significantly reduced in MCF-7 and MDA-MB-231 cells incubated with 40 and 80 μM concentrations of GL for 24 hours. In addition, GL induced the initiation of autophagy in MDA-MB-231 and MCF-7 cells, as evidenced by enhanced levels of Beclin-1, LC3 and p62 expression. At the same time, this study showed that GL induced cell cycle arrest in the G2/M phases.
Conclusion: GL has anti-proliferative activity by arresting the cell cycle at the G2/M phase and accelerates cell death by inducing autophagy, particularly in aggressive breast cancers that are resistant to treatment..
Anahtar Kelimeler
Destekleyen Kurum
Erciyes University scientific research projects coordination unit
Proje Numarası
TSA-2020-10396
Teşekkür
The experimental stages of the study took place at the Erciyes University genome and stem cell center (GENKOK).
Kaynakça
- 1. Farghadani R, Naidu R. Curcumin: Modulator of Key Molecular Signaling Pathways in Hormone-Independent Breast Cancer. Cancers 2021; 13(14): 3427.
- 2. Ensenyat-Mendez M, Llinàs-Arias P, Orozco JIJ, Íñiguez-Muñoz S, Salomon MP, Sesé B, DiNome ML, Marzese DM. Current Triple-Negative Breast Cancer Subtypes: Dissecting the Most Aggressive Form of Breast Cancer. Front Oncol 2021; 11: 681476.
- 3. Musgrove EA, Sutherland RL. Biological determinants of endocrine resistance in breast cancer. Nat Rev Cancer 2009; 9:631–43.
