Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin
Öz
Purpose: We aimed the potential protective effects of curcumin (CUR) and beta-carotene (BC) against cisplatin (CIS)-induced ovarian damage using histological and immunohistochemical methods.
Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 ml/kg sesame oil by gavage; the CIS group 5 mg/kg CIS; the CUR group 200 mg/kg CUR; the BC group 100 mg/kg BC; the CUR + CIS group, CIS after administration of CUR; the BC + CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone (AMH) and nuclear factor-kappa B (NF-κB) was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method.
Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR + CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC.
Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.
Anahtar Kelimeler
Anti-Mullerian hormon, Apoptosis, beta-karoten, cisplatin, kurkumin, NF-κB
Destekleyen Kurum
This study was supported by grants from the Erciyes University Scientific Research Projects Unit
Proje Numarası
project no. TYL-2016-7069
Kaynakça
- Hansen KR, Knowlton NS, Thyer AC, Charleston JS, Soules MR, Klein NA. A new model of reproductive aging: the decline in ovarian non-growing follicle number from birth to menopause. Hum Reprod. 2008;23:699–708.
- Rossi V, Lispi M, Longobardi S, Mattei M, Di Rella F, Salustri A et al. LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse. Cell Death Differ. 2017;24:72–82.
- Brown A, Kumar S, Tchounwou P. Cisplatin-based chemotherapy of human cancers. J Cancer Sci Ther. 2011;11:97–103.
- Gouveia BB, Barberino RS, Menezes VG, Monte APO, Silva RLS, Palheta RC Jr et al. Amburana cearensis leaf extract protects against cisplatin-induced ovarian damage through regulation of p-PTEN and p-Akt proteins in mice. Iran J Basic Med Sci. 2022;25:683-9.
- Gąsiorkiewicz BM, Koczurkiewicz-Adamczyk P, Piska K, Pękala E. Autophagy modulating agents as chemosensitizers for cisplatin therapy in cancer. Invest New Drugs. 2021;39:538-63.
- Anderson RA, Clatot F, Demeestere I, Lambertini M, Morgan A, Nelson SM et al. Cancer survivorship: Reproductive health outcomes should be included in standard toxicity assessments. Eur J Cancer. 2021;144:310-16.
- Pandya U, Saini MK, Jin GF, Awasthi S, Godley BF, Awasthi YC. Dietary curcumin prevents ocular toxicity of naphthalene in rats. Toxicol Lett. 2000;115:195-204.
- Farombi EO, Ekor M. Curcumin attenuates gentamicin-induced renal oxidative damage in rats. Food Chem Toxicol. 2006;44:1443-8.
- Menon VP, Sudheer AR. Antioxidant and anti-inflammatory properties of Curcumin. Adv Exp Med Biol. 2007;595:105–25.
- Devasena T, Rajasekaran KN, Menon VP. Bis‑1,7‑(2‑hydroxyphenyl)‑hepta‑1,6‑diene‑3,5‑dione (a curcumin analog) ameliorates DMH‑induced hepatic oxidative stress during colon carcinogenesis. Pharmacol Res. 2002;46:39‑45.
