Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Ayşe Ceyhan; Münevver Baran; Pınar Alişan Suna; Özge Cengiz Mat; Arzu Yay

doi:10.17826/cumj.1345688

TR EN

Kurkumin ve beta-karotenin sisplatinin neden olduğu over hasarı üzerindeki etkilerinin karşılaştırılması

Öz

Amaç: Cisplatin (CIS) kaynaklı over hasarına karşı kurkumin (CUR) ve beta-karotenin (BC) potansiyel koruyucu etkilerinin histolojik ve immünohistokimyasal yöntemlerle araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışmada, rastgele yedi gruba ayrılan 56 dişi Wistar albino sıçan kullanıldı. Kontrol sıçanları herhangi bir tedavi görmedi; sham grubuna gavajla 1 ml/kg susam yağı verildi; CIS grubuna 5 mg/kg CIS; CUR grubuna 200 mg/kg CUR; BC grubuna 200 mg/kg BC; CUR + CIS grubuna, CUR uygulamasından sonra CIS uygulandı; BC + CIS grubu, BC ile ön işleme tabi tutuldu, ardından CIS uygulandı. Tüm grupların overleri son uygulamadan beş gün sonra eksize edildi. Elde edilen dokularda, histopatolojik değerlendirme yapıldı ve folikül rezervi hesaplandı. Anti-Mullerian hormon (AMH) ve nükleer faktör-kappa B (NF-κB) ekspresyonu immünohistokimyasal olarak saptandı. Apoptoz ise, TUNEL yöntemi kullanılarak değerlendirildi. Bulgular: CUR ve BC'nin, CIS'in neden olduğu azalan primordial, primer, preantral, sekonder ve tersiyer folikül sayılarına karşı koruyucu olduğu gözlendi. AMH immünoreaktivitesi, kontrol ve CUR + CIS grupları için hemen hemen aynıydı. CUR ve BC gruplarında kontrollere kıyasla over NF-κB ekspresyon seviyelerini düşürdü. TUNEL pozitif apoptotik hücre sayıları, CUR'nin BC'den daha güçlü bir anti-apoptotik etki gösterdiğini gösterdi. Sonuç: CIS’nin neden olduğu over hasarına karşı CUR'nin BC'den daha fazla antioksidan ve antiinflamatuar etkiye sahip olduğunu gösterilmiştir.

Anahtar Kelimeler

Anti-Mullerian hormone , apoptosis , beta-karoten , cisplatin , kurkumin , NF-κB

Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Abstract

Purpose: We aimed the potential protective effects of curcumin (CUR) and beta-carotene (BC) against cisplatin (CIS)-induced ovarian damage using histological and immunohistochemical methods. Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 ml/kg sesame oil by gavage; the CIS group 5 mg/kg CIS; the CUR group 200 mg/kg CUR; the BC group 100 mg/kg BC; the CUR + CIS group, CIS after administration of CUR; the BC + CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone (AMH) and nuclear factor-kappa B (NF-κB) was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method. Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR + CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC. Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.

Keywords

Anti-Mullerian hormon , Apoptosis , beta-karoten , cisplatin , kurkumin , NF-κB

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Bölüm

Araştırma Makalesi

Yazarlar

Ayşe Ceyhan
0000-0002-4289-2305
Türkiye

Münevver Baran
0000-0003-0369-1022
Türkiye

Pınar Alişan Suna
0000-0002-6861-416X
Türkiye

Özge Cengiz Mat
0000-0003-4638-6116
Türkiye

Arzu Yay ^*
0000-0002-0541-8372
Türkiye

Yayımlanma Tarihi

29 Aralık 2023

Gönderilme Tarihi

25 Ağustos 2023

Kabul Tarihi

16 Kasım 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 48 Sayı: 4

DOI

https://doi.org/10.17826/cumj.1345688

IZ

https://izlik.org/JA24EH92YM

MLA

Ceyhan, Ayşe, vd. “Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin”. Cukurova Medical Journal, c. 48, sy 4, Aralık 2023, ss. 1248-57, doi:10.17826/cumj.1345688.

Cited By

Oxidative Stress, Inflammation, and Antioxidant Strategies in Cervical Cancer—A Narrative Review

International Journal of Molecular Sciences

https://doi.org/10.3390/ijms26104961

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Kurkumin ve beta-karotenin sisplatinin neden olduğu over hasarı üzerindeki etkilerinin karşılaştırılması

Öz

Anahtar Kelimeler

Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Oxidative Stress, Inflammation, and Antioxidant Strategies in Cervical Cancer—A Narrative Review