Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue
Öz
Purpose. In this study, the expression levels of METTL3, METTL14; and WTAP genes involved in the N6-methyladenosine (m6A) pathway, regulating cellular processes including cell renewal, differentiation, invasion; and apoptosis, were investigated in duodenal tissue after sleeve gastrectomy (SG).
Material and Methods: Duodenal biopsies were obtained from 24 obese patients before (BG) and 6 months after (AG) sleeve gastrectomy. Pathological evaluation was performed by H&E staining. Expression levels of METTL14, METTL3, and WTAP genes were analysed by Real-Time PCR.
Results: The METTL14 gene was significantly increased after surgery (BG-AG Median (Q1-Q3): 0.36 [0.07-1.52]-9.83 [0.20-68.12]). METTL3 (BG-AG Median (Q1-Q3): 0.49 [0.32-0.84]-0.67 [0.36-20.5]) and WTAP (BG-AG Median (Q1-Q3): 0.95 [0.17-7.65]-5.54 [2.34-66.07]) gene expressions were not altered. However, a strong positive (r=0.692) and significant relationship was found between METTL3 and WTAP. Chronic inflammation 81.32 %, intestinal metaplasia 10.54 %, activity 68.88 %, atrophy 5.32 %. No malignant findings were detected.
Conclusion: For the first time, we demonstrated that sleeve gastrectomy may increase the expression of the METTL14 gene involved in the m6A-methyladenosine pathway in duodenum. This alteration may lead to gastrointestinal tract diseases.
Anahtar Kelimeler
Sleeve Gastrectomy, Bariatric Surgery, Epigenetic, Duodenum, N6-Methyladenosine
Destekleyen Kurum
This study was supported by the authors.
Kaynakça
- Zhai Z, Li G, Tao Y, Wang Z, Han J. Sleeve gastrectomy plus uncut jejunojejunal bypass for the treatment of obesity and type 2 diabetes. Chin Med J. 2022;135:2240-1.
- Gagner M. Effect of sleeve gastrectomy on type 2 diabetes as an alternative to Roux-en-Y gastric bypass: a better long-term strategy. Surg Obes Relat Dis. 2015;11:1280-1.
- Andrea GI, Ana BC. Obesity-related epigenetic changes after bariatric surgery. Front Endocrinol. 2019;10:232.
- González MM, Gracia MMN, Lourdes GS, Eduardo GF, Francisco T, Sonsoles M. Decreased blood pressure is related to changes in NF-kB promoter methylation levels after bariatric surgery. Surg Obes Relat Dis. 2018;14:1327-34.
- Assem S, Abdelbaki TN, Mohy-E Dine SH, Ketat AF, Abdelmonsif DA. Serpine-1 gene methylation and protein as molecular predictors of laparoscopic sleeve gastrectomy outcome. Obes Surg. 2020;30:2620–30.
- Beisani M, Pappa S, Moreno P, Martínez E, Tarasco J, Granada ML et al. Laparoscopic sleeve gastrectomy induces molecular changes in peripheral white blood cell. Clin Nutr. 2020;39:592-98.
- He L, Li J, Wang X, Ying Y, Xie H, Yan H et al. The dual role of N6-methyladenosine modification of RNAs is involved in human cancers. J Cell Mol Med. 2018;22:4630–9.
- Batista PJ. The RNA modification N (6)-methyladenosine and Its implications in human disease. Genomics Proteomics Bioinform. 2017;15:154–63.
- Xie B, Deng Z, Pan Y, Fu C, Fan S, Tao Y. Post-transcriptional regulation DPC4 gene by miR-190 in colorectal cancer cells. J Cancer Res Ther. 2018;14:838–43.
- Li J, Meng S, Xu M, Wang S, He L, Xu X et al. Downregulation of N (6)-methyladenosine binding YTHDF2 protein mediated by miR-493-3p suppresses prostate cancer by elevating N (6)-methyladenosine levels. Oncotarget. 2018;9:3752–64.
