Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue

Badel Arslan; Hakan Seyit; Murat Eser Akyürek; Serdal Arslan; Damlanur Sakız; Çağatay Han Türkseven

doi:10.17826/cumj.1370487

EN TR

Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue

Abstract

Purpose. In this study, the expression levels of METTL3, METTL14; and WTAP genes involved in the N6-methyladenosine (m6A) pathway, regulating cellular processes including cell renewal, differentiation, invasion; and apoptosis, were investigated in duodenal tissue after sleeve gastrectomy (SG). Material and Methods: Duodenal biopsies were obtained from 24 obese patients before (BG) and 6 months after (AG) sleeve gastrectomy. Pathological evaluation was performed by H&E staining. Expression levels of METTL14, METTL3, and WTAP genes were analysed by Real-Time PCR. Results: The METTL14 gene was significantly increased after surgery (BG-AG Median (Q1-Q3): 0.36 [0.07-1.52]-9.83 [0.20-68.12]). METTL3 (BG-AG Median (Q1-Q3): 0.49 [0.32-0.84]-0.67 [0.36-20.5]) and WTAP (BG-AG Median (Q1-Q3): 0.95 [0.17-7.65]-5.54 [2.34-66.07]) gene expressions were not altered. However, a strong positive (r=0.692) and significant relationship was found between METTL3 and WTAP. Chronic inflammation 81.32 %, intestinal metaplasia 10.54 %, activity 68.88 %, atrophy 5.32 %. No malignant findings were detected. Conclusion: For the first time, we demonstrated that sleeve gastrectomy may increase the expression of the METTL14 gene involved in the m6A-methyladenosine pathway in duodenum. This alteration may lead to gastrointestinal tract diseases.

Keywords

Sleeve Gastrectomy , Bariatric Surgery , Epigenetic , Duodenum , N6-Methyladenosine

Duodenal dokuda N6-metiladenozin modifikasyonu üzerine sleeve gastrektomi etkileri

Öz

Amaç: Bu retrospektif çalışmada açık kalp cerrahisi geçiren 80 yaş üstü hastaların operasyon öncesi albümin değeri kullanılarak hesaplanan geriatrik nutrisyonel risk indeksinin, ameliyat sonrası mortalite oranını tahmin edebilme gücü ortaya konulmaya çalışıldı. Gereç ve Yöntem: Temmuz 2019 – Eylül 2022 tarihleri arasında koroner bypass ve kalp kapağı değişimi nedeniyle açık elektif kalp cerrahisi uygulanan toplam 22 hasta (13 erkek, 9 kadın; ortalama yaş: 82,1±2,4 yıl; dağılım, 80-88 yıl) geriatrik nutrisyonel risk indeksi ile retrospektif olarak incelendi. Bu indekse göre iki grup oluşturuldu. 98 ve altındaki değerler riskli, 99 ve üstündeki değerler risksiz grup olarak belirlendi. Riskli grupta 6 hasta vardı. Diğer 16 hasta risksiz grupta bulunuyordu. Tüm hasta grubu cerrahi işlemler, cinsiyet, yaş, vücut kütle indeksi (kg/m2), atrial fibrilasyon, diyabet, hipertansiyon, sigara içiciliği ve operatif özellikler yönüyle karşılaştırıldı. Bulgular: Çalışmada geriatrik nutrisyonel risk indeksine göre 6 riskli (%27,3) ve 16 risksiz (%72,7) hasta incelendi ve risk varlığı tespit edilen hastalarda mortalite oranı yüksek (%66,7) bulundu. Risk grubundaki hastalar, risk grubu olmayanlara kıyasla daha düşük vücut kitle indeksi ve albümin değerlerine sahipti (sırasıyla 25,0±1,8 ve 30,1±2,4; 28,1±2,4 ve 36,7±3,1). Ayrıca, ortalama entübasyon değerlerinin yüksek bulunduğu belirlendi (11,2±4,2). Diğer parametreler ile risk varlığı arasında anlamlı bir farklılığa rastlanılmadı. Sonuç: Açık kalp cerrahisi öncesi değerlendirilen geriatrik nutrisyonel risk indeksi, operasyon sonrası mortalite oranını öngörmede güçlü bir belirleyici olarak kullanılabilir.

Anahtar Kelimeler

Geriatrik nutrisyonel risk indeksi , oktogenarian , albümin , malnütrisyon

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Gastroenteroloji Cerrahisi , Tıbbi Genetik (Kanser Genetiği hariç)

Bölüm

Araştırma Makalesi

Yazarlar

Badel Arslan
0000-0002-0004-3567
Türkiye

Hakan Seyit
0000-0003-3708-5370
Türkiye

Murat Eser Akyürek
0000-0003-0740-7738
Türkiye

Serdal Arslan
0000-0002-3921-8061
Türkiye

Damlanur Sakız
0000-0002-2051-1049
Türkiye

Çağatay Han Türkseven ^*
0000-0002-0584-0661
Türkiye

Yayımlanma Tarihi

29 Aralık 2023

Gönderilme Tarihi

3 Ekim 2023

Kabul Tarihi

15 Aralık 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 48 Sayı: 4

DOI

https://doi.org/10.17826/cumj.1370487

IZ

https://izlik.org/JA89SF67JB

MLA

Arslan, Badel, vd. “Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue”. Cukurova Medical Journal, c. 48, sy 4, Aralık 2023, ss. 1365-73, doi:10.17826/cumj.1370487.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue

Abstract

Keywords

Duodenal dokuda N6-metiladenozin modifikasyonu üzerine sleeve gastrektomi etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster