Investigation of the protective effect of selenium supplementation on renal function in cisplatin-administered rats
Öz
Purpose: Selenium is an important antioxidant and anticarcinogen with the ability to protect cells from oxidative stress, a significant marker of cisplatin-induced toxicity. This study aimed to reveal the effect of selenium on free radicals in cisplatin-induced nephrotoxicity by examining changes in creatinine, neutrophil gelatinase-associated lipocalin (NGAL), and galectin-3, which are associated with kidney damage.
Materials and Methods: Twenty-four Wistar albino rats, aged 60 days, were equally divided into four groups: control, cisplatin, selenium, and cisplatin+selenium. The experiment started on the 39th day after the rats were born. Controls were intraperitoneally administered a single dose of physiological saline. Rats in the selenium and cisplatin+selenium groups were administered 1 mg/kg of selenium by gastric gavage per day for 21 days. The rats in the cisplatin and cisplatin+selenium groups were intraperitoneally administered 7.5 mg/kg of cisplatin on the 57th day. The experiment was terminated 3 days after single-dose administration. Tissue samples were analyzed using the ICP-MS method for selenium, the biochemical method for plasma creatinine, and the ELISA method for NGAL and galectin-3.
Results: Kidney tissue selenium levels were significantly higher in the selenium-supplemented groups (control;146.8 ± 10.8 ng/dl, selenium;520.2 ± 31.2 ng/dl, cisplatin;140 ± 6.4 ng/dl; cisplatin + selenium; 363.4 ± 33.6 ng/dl). Plasma creatinine levels were statistically significantly higher in the cisplatin-administered groups (control; 0.32 ± 0.01 mg/dl, selenium; 0.32 ± 0.01 mg/dl, cisplatin; 0.47 ± 0.02 mg/dl; cisplatin + selenium; 0.45 ± 0.04). There was no difference in kidney tissue NGAL levels; however, galectin-3 levels were significantly increased in the cisplatin group compared with the other groups. This increase was lower in the cisplatin+selenium group than in the cisplatin group. Heart tissue NGAL and galectin-3 levels were higher in the cisplatin group.
Conclusion: Selenium supplementation may have a healing effect on the nephrotoxicity and cardiotoxicity caused by cisplatin, as indicated by changes in creatinine, NGAL, and galectin-3 levels.
Anahtar Kelimeler
Destekleyen Kurum
This work was financially supported by the Scientific Research Projects (BAP) Department of Selçuk University (23401079).
Proje Numarası
23401079
Etik Beyan
This study was approved by the Animal Experiments Ethics Committee of Selçuk University Experimental Medicine Application and Research Center with decision number 2023-23, dated April 28, 2023.
Kaynakça
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