Microparticle profile during painful crisis and steady state in children with sickle cell anemia
Abstract
Purpose: Sickle cell anemia is a disease characterized by hemolytic anemia, hypercoagulopathy, and painful crises. Microparticles are 0.1-1 µm sized membrane particles derived during cellular activation or apoptotic phases of the cell cycle. In this study, we investigated the role of microparticles on clinical state and prognosis during painful crisis and steady state in children with sickle cell anemia.
Materials and Methods: Patients with sickle cell anemia who were followed up in Çukurova University Pediatric Hematology Department and presented with a painful crisis were included in the study. Children without any systemic disease were included in the control group. Total microparticle levels, erythrocyte (CD235a), endothelial (CD106), and monocyte (CD14) microparticle levels, and tissue factor expressing (CD142) microparticle levels were analyzed.
Results: A total of 29 patients with sickle cell anemia who presented with a painful crisis were included in the study. In addition, blood samples were collected from 26 of these patients in a steady state. Blood samples were obtained from 18 healthy children as the control group. Total microparticle levels were significantly higher in sickle cell anemia patients in painful crises than in control group. Erythrocyte and monocyte microparticle levels were significantly higher in patients with a painful crisis than in a steady state. Endothelial and tissue factor expressing microparticle levels were higher during a painful crisis than steady state, although not at statistically significant levels. Microparticle levels were lower in patients with hydroxyurea treatment than those without, although it was not a statistically significant difference.
Conclusion: Total microparticles as well as erythrocyte and monocyte microparticles were high in sickle cell anemia patients during a painful crisis. Studies involving larger numbers of patients are needed to better understand the role of microparticles in the pathophysiology of sickle cell anemia and their association with painful crises.
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