Relationship between Klotho gene methylation level and diet habit
Öz
Purpose: This study aimed to determine the relationship between the methylation level of the Klotho gene and nutritional habits.
Materials and Methods: From our healthy sample group consisting of 20 people, two groups were created: 10 people fed with carbohydrates and 10 people had protein. Initially, a food consumption frequency determination form was administered as a survey to individuals. Based on the results of this survey, the amounts of food consumed by the participants (g/cc) were determined. According to the findings of the survey, two groups were formed: those classified as carbohydrate consumers (individuals consuming 33% or more of their diet from carbohydrates) and those classified as protein consumers (individuals consuming 17% or more of their diet from protein). Methylation level of Klotho gene in blood samples of individuals; DNA isolation, RT-PCR and Bisulfite Modification were examined.
Results: In the carbohydrate diet group; there was a very strong and inverse correlation between fat and methylation percentages (r = -0.765, p = 0.05). There was a strong correlation between the percentages of carbohydrate and methylation (r = 0.778, p = 0.004). A strong correlation was also found between BMI and methylation percentage (r = 0.712, p = 0.01). There was a strong inverse correlation between cholesterol and methylation percentages (r =-0.556, p = 0.04). In the protein diet group, there was a strong inverse correlation between BMI and methylation (r =-0.635, p = 0.024).
Conclusion: As a result of the analysis, the Klotho gene methylation percentage (33%) in individuals with a carbohydrate-based diet was found to be higher than that in individuals with a protein-based diet (17%). The data obtained indicate that as carbohydrate consumption increases, the methylation level of the Klotho gene also rises.
Anahtar Kelimeler
Destekleyen Kurum
This research was supported by Necmettin Erbakan University Scientific Research Projects Coordination with project number 181318008.
Etik Beyan
Decision numbered 2018/1279 was taken on 16.03.2018 at the 65th meeting.
Teşekkür
This research was supported by Necmettin Erbakan University Scientific Research Projects Coordination with project number 181318008.
Kaynakça
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