Assessment of antioxidant efficacy of saffron-withania somnifera herbal gel against oral ulcers: an invitro study
Öz
Abstract
Purpose: Recurrent aphthous stomatitis (RAS) is the most commonly seen ulcerative lesion of the oral mucosa. The lesions are generally recurrent, painful and can affect the quality of life. The aim of this study was to evaluate the efficacy of herbal extracts of saffron, withania somnifera, Dry ginger, Tulsi as an antioxidant agent in RAS.
Materials and Methods: 1g powder of tulsi, withania somnifera, dry ginger was mixed with 100ml of distilled water separately and boiled in a heating mantle at 50 to 60 degree for 15 minutes. Anti-inflammatory, antimicrobial, antioxidant,cytotoxic activities were tested by adding 5 ml extract to empty gel containing a combination of carboxylate methyl cellulose 5g, carbapel 5g. Antioxidant property of withania somnifera, saffron, dry ginger and tulsi were evaluated using DPPH assay, H2O2 assay and FRAP assay.
Results: Our study revealed highest zone of inhibition of 90.22, 84.3, 88.93% at a concentration of 50 microgram per milliliter which is near to that of standard value
Conclusion: This study gives an insight that the naturally occurring constituents of medicinal herbs will be able to resolve oral ulcers thereby improving the quality of life of the patients. Hence, herbal medicines can be used as an adjunct to synthetic drugs available commercially.
Anahtar Kelimeler
Withania somnifera, Tulsi, Dry ginger, Saffron, ulcer, medicine, quality of life
Proje Numarası
SRB/SDC/OMED-2204/23/136
Etik Beyan
Scientific Review Board- Saveetha Dental College number for the study entitled "ASSESSMENT OF ANTIOXIDANT PROPERTY OF SAFFRON BASED HERBAL GEL AGAINST RECURRENT APHTHOUS STOMATITIS- AN INVITRO STUDY " is SRB/SDC/OMED-2204/23/136
Kaynakça
- Akintoye SO, Greenberg MS. Recurrent aphthous stomatitis. Dent Clin North Am. 2014;58:281-97..
- Srivastava A, Gc S, Pathak S, Ingle E, Kumari A, Shivakumar S et al. Evidence-based effectiveness of herbal treatment modality for recurrent aphthous ulcers - A systematic review and meta-analysis. Natl J Maxillofac Surg. 2021;12:303-10.
- Manwar J, Mahadik K, Sathiyanarayanan L, Paradkar A, Patil S. Comparative antioxidant potential of Withania somnifera based herbal formulation prepared by traditional and non-traditional fermentation processes. Integr Med Res. 2013;2:56-61.
- Kumbukgolla WW, Attanayake AM, Jayaweera JA. Antibacterial and antioxidant activity of different extracts obtained from Withania somnifera (Ashwagandha). International Journal of Ayurvedic Medicine,2021;12:873-7.
- Rahaiee S, Moini S, Hashemi M, Shojaosadati SA. Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): a review. J Food Sci Technol. 2015;52:1881-8.
- Zhang A, Shen Y, Cen M, Hong X, Shao Q, Chen Y. Polysaccharide and crocin contents, and antioxidant activity of saffron from different origins. Ind Crops Prod. 2019;133:111-7.
- Chaudhary A, Sharma S, Mittal A, Gupta S, Dua A. Phytochemical and antioxidant profiling of Ocimum sanctum. J Food Sci Technol. 2020;57:3852-63.
- Mustafa I, Chin NL. Antioxidant properties of dried ginger (Zingiber officinale Roscoe) var. Bentong. Foods. 2023;12:178.
- Porter SR, Scully C, Pedersen A. Recurrent aphthous stomatitis. Crit Rev Oral Biol Med. 1998;9(3):306-21.
- Pandharipande R, Chandak R, Sathawane R, Lanjekar A, Gaikwad R, Khandelwal V. To evaluate efficiency of curcumin and honey in patients with recurrent aphthous stomatitis: a randomized clinical controlled trial. Int J Res Rev. 2019;6:449-55.
