Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Emine Kılıç Toprak; Tarık Şengöz; Olga Yaylalı; Özgen Kılıç Erkek; Yasin Özdemir; Burak Oymak; Hande Şenol; Z. Melek Küçükatay; Vural Küçükatay; Doğangün Yüksel

doi:10.17826/cumj.1589549

EN TR

Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Abstract

Purpose: The purpose of this study was to ascertain how radioactive iodine therapy (RAIT). affected the DNA damage in the lymphocytes of differentiated thyroid cancer (DTC) patients. Materials and Methods: Our study included 21 DTC patients in total, with a mean age of 45.09±10.02 years. Six (28.6%) of the 21 patients had a low dose of RAIT (≤30 mCi), eleven (52.4%) received a moderate dose of ablation (31 to 100 mCi), and four (%19) received a high dose (>100 mCi). Before, one week, and six months following treatment, venous blood samples were obtained from each patient. DNA damage was evaluated using the COMET assay. For a quantitative analysis of DNA damage, a number of characteristics were assessed, including head length, tail length, head intensity, tail intensity, and tail moment. Results: Compared to before treatment (23.83±9.82), tail intensity levels were considerably lower after treatment (1st week and 6th months) (18.30±6.48 vs. 18.06±5.58). Following therapy (1st week and 6th months), the head intensity values were statistically substantially higher (81.69±6.48 vs. 81.93±5.58) than they were prior to treatment (76.16±9.82). Six months after therapy, the tail moment was lower than it was before treatment (5.24±2.51 vs. 8.04±3.86). Conclusion: Treatment has a beneficial effect on peripheral blood cells, as seen by the increase in head intensity and decrease in tail intensity and moment after treatment. By triggering repair mechanisms, RAIT may have activated protective effects on the DNA of lymphocytes.

Keywords

Differentiated thyroid cancer , I-131 , radioactive iodine theraphy , DNA damage , COMET assay

Diferansiye tiroid kanseri olan hastalarda I-131 ablasyonu/metastaz tedavisinin DNA hasarı parametreleri üzerindeki etkileri: pilot çalışma

Öz

Amaç: Bu çalışmanın amacı, radyoaktif iyot tedavisinin (RAIT) farklılaşmış tiroid kanseri (DTC) hastalarının lenfositlerindeki DNA hasarını nasıl etkilediğini belirlemekti. Gereç ve Yöntem: Çalışmamıza toplam 21 DTC hastası dahil edildi ve yaş ortalamaları 45.09±10.02 yıldı. 21 hastanın altısı (%28.6) düşük doz RAIT (≤30 mCi) aldı, on biri (%52.4) orta doz ablasyon (31 ila 100 mCi) aldı ve dördü (%19) yüksek doz (>100 mCi) aldı. Tedaviden önce, bir hafta ve altı ay sonra her hastadan venöz kan örnekleri alındı. DNA hasarı COMET testi kullanılarak değerlendirildi. DNA hasarının kantitatif analizi için baş uzunluğu, kuyruk uzunluğu, baş yoğunluğu, kuyruk yoğunluğu ve kuyruk momenti dahil olmak üzere çeşitli parametreler değerlendirildi. Bulgular: Kuyruk yoğunluğu değerleri tedaviden sonra (1 hafta ve 6 ay) tedavi öncesine (23,83 ± 9,82) kıyasla önemli ölçüde daha düşüktü (18,30 ± 6,48'e karşı 18,06 ± 5,58). Baş yoğunluğu değerleri tedaviden sonra (1. hafta ve 6. ay) (81.69±6.48'e karşı 81.93±5.58) tedavi öncesine (76.16±9.82) göre istatistiksel olarak anlamlı derecede daha yüksekti. Kuyruk momenti tedaviden 6 ay sonra tedavi öncesine göre daha düşüktü (5.24±2.51'e karşı 8.04±3.86). Sonuç: Tedaviden sonra baş yoğunluğunda artış ve kuyruk yoğunluğunda ve momentinde azalma, tedavinin periferik kan hücreleri üzerinde olumlu bir etkiye sahip olduğunu göstermiştir. RAIT'in onarım mekanizmalarını aktive ederek lenfosit DNA'sı üzerinde koruyucu etkileri aktive etmiş olabileceği düşünülebilir.

Anahtar Kelimeler

Farklılaşmış tiroid kanseri , I-131 , radyoaktif iyot tedavisi , DNA hasarı , COMET analizi

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Bölüm

Araştırma Makalesi

Yazarlar

Emine Kılıç Toprak ^*
0000-0002-8795-0185
Türkiye

Tarık Şengöz
0000-0003-2621-7585
Türkiye

Olga Yaylalı
0000-0002-4920-865X
Türkiye

Özgen Kılıç Erkek
0000-0001-8037-099X
Türkiye

Yasin Özdemir
0000-0002-7562-0744
Türkiye

Burak Oymak
0000-0002-2887-0783
Türkiye

Hande Şenol
0000-0001-6395-7924
Türkiye

Z. Melek Küçükatay
0000-0002-9366-0205
Türkiye

Vural Küçükatay
0000-0002-6850-6281
Türkiye

Doğangün Yüksel
0000-0003-0983-2834
Türkiye

Yayımlanma Tarihi

30 Eylül 2025

Gönderilme Tarihi

22 Kasım 2024

Kabul Tarihi

21 Haziran 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 50 Sayı: 3

DOI

https://doi.org/10.17826/cumj.1589549

IZ

https://izlik.org/JA79ME58NM

MLA

Kılıç Toprak, Emine, vd. “Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study”. Cukurova Medical Journal, c. 50, sy 3, Eylül 2025, ss. 609-16, doi:10.17826/cumj.1589549.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Abstract

Keywords

Diferansiye tiroid kanseri olan hastalarda I-131 ablasyonu/metastaz tedavisinin DNA hasarı parametreleri üzerindeki etkileri: pilot çalışma

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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