Impact of genistein on androgen-independent PC3 prostate cancer cells

Nur Özten Kandaş; Şule Terzioğlu Uşak; Beyza Göncü

doi:10.17826/cumj.1633575

EN TR

Impact of genistein on androgen-independent PC3 prostate cancer cells

Abstract

Purpose: This study evaluates genistein’s effects on cell survival, migration, apoptosis, reactive oxygen species (ROS) generation, and Manganese Superoxide Dismutase (MnSOD) expression in androgen-independent PC3 prostate cancer cells, providing insight into its potential as an adjuvant therapy for castration-resistant prostate cancer (CRPC). Materials and Methods: Cells were treated with vehicle only and 0.5, 2.5, 5, 10, and 50 µM genistein concentrations for 24 and 48 hours. Cell proliferation assay, wound healing assay, ROS measurement, apoptosis detection, and MnSOD protein expression analysis were performed. Results: The findings indicate a biphasic effect of genistein on PC3 cell survival. Lower to physiologically relevant concentrations (0.5–10 µM) exhibit a modest stimulatory effect, whereas a higher, pharmacologically achievable concentration (50 µM) leads to a time-dependent decline in survival and a significant restriction on migration. In vehicle-treated cells, 77% remained viable, with low early (3.65%) and late apoptosis (16.35%). Lower genistein concentrations (0.5–10 µM) caused a slight increase in apoptosis and a modest decline in viability. However, at 50 µM, only 18.7% of cells remained viable, while 74.25% underwent late apoptosis or cell death. Conclusion: These findings demonstrate that genistein, particularly at higher concentrations, inhibits androgen-independent PC3 cell growth through apoptosis induction, MnSOD regulation, and elevated oxidative stress.

Keywords

androgen-independent prostate cancer cells , genistein , cell migration , ROS production , cell survival , apoptosis.

Genisteinin androjen-bağımsız PC3 prostat kanseri hücreleri üzerindeki etkisi

Öz

Amaç: Bu çalışma, genisteinin andojen-bağımsız prostat kanser hücrelerinde hücre sağkalımını ve migrasyonunu, apoptozu, reaktif oksijen türleri (ROS) oluşumunu, antioksidan protein Manganez Süperoksit Dismutaz (MnSOD) ekspresyonunu nasıl etkilediğini göstermeyi ve özellikle kastrasyona dirençli prostat kanser adjuvan tedavisi olası kullanımına ışık tutmayı amaçlamaktadır. Gereç ve Yöntem: Hücreler, 24 ve 48. saatlerde genistein bulunmayan ve 0.5, 2.5, 5, 10 ve 50 µM konsantrasyonlarda genistein bulunan ortamlarda büyütüldü. Sonuçları değerlendirmek için hücre proliferasyon analizi, hücre migrasyon analizi, reaktif oksijen türleri üretim ölçümü, apoptoz tespiti ve MnSOD protein ekspresyon analizi yapıldı. Bulgular: Bulgularımız, genisteinin PC3 hücreleri üzerinde bifazik etkileri olduğunu göstermiştir. Özellikle daha düşük-fizyolojik konsantrasyonlarda (0,5-10 µM) ılımlı bir uyarıcı özellik gösterdiği, daha yüksek, farmakolojik (50 µM) konsantrasyonda hücre sağkalımında düşüş ve hücre göçünde önemli bir kısıtlamaya sebep olduğu saptanmıştır. Sonuç: Analiz sonuçları genisteinin, özellikle yüksek konsantrasyonda, androjen-bağımsız PC3 prostat kanseri hücrelerinin büyümesini, apoptoz indüksiyonu, MnSOD regülasyonu ve yüksek oksidatif stres gibi süreçler yoluyla inhibe ettiğini göstermektedir.

Anahtar Kelimeler

androjen-bağımsız prostat kanseri hücreleri , genistein , hücre göçü , ROS üretimi , hücre sağkalımı , apoptoz

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Üroloji , Kanser Hücre Biyolojisi

Bölüm

Araştırma Makalesi

Yazarlar

Nur Özten Kandaş ^*
0000-0002-0441-8397
United States

Şule Terzioğlu Uşak
0000-0002-4594-2697
Türkiye

Beyza Göncü
0000-0001-6026-8218
Türkiye

Yayımlanma Tarihi

31 Mart 2025

Gönderilme Tarihi

5 Şubat 2025

Kabul Tarihi

24 Mart 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 50 Sayı: 1

DOI

https://doi.org/10.17826/cumj.1633575

IZ

https://izlik.org/JA69MK54LR

MLA

Özten Kandaş, Nur, vd. “Impact of genistein on androgen-independent PC3 prostate cancer cells”. Cukurova Medical Journal, c. 50, sy 1, Mart 2025, ss. 231-40, doi:10.17826/cumj.1633575.

Cited By

Genistein Nanopreparations: Recent Advances in preparation, bioactivities, and Applications

Journal of Cluster Science

https://doi.org/10.1007/s10876-025-02960-6

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Impact of genistein on androgen-independent PC3 prostate cancer cells

Abstract

Keywords

Genisteinin androjen-bağımsız PC3 prostat kanseri hücreleri üzerindeki etkisi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Genistein Nanopreparations: Recent Advances in preparation, bioactivities, and Applications