Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells
Öz
Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum (Savi) DC. plants in breast cancer cells.
Materials and Methods: MDA-MB-231 triple-negative breast cancer (TNBC) cells were used to demonstrate reactive oxygen species (ROS) production effects and histone deacetylase (HDAC) inhibitory activity of flavonoids [5,3',4'-trihydroxy-7-methoxy isoflavone (Santal) (1), 5,5'',7''-trihydroxy-3,7-dimethoxy-4',4'''-O-biflavone (2), 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside (3), (8,3',4',5'-tetrahydroxy-7-O-α-glycosyl isoflavone)-6-8''-(4'''-hydroxy-7''-O-α-glycosyl isoflavone) biflavone (4)] isolated from C. italicum.
Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mg/ml. Compound 2 at 0.06 mg/ml, and compounds 2 and 4 at 0.08 and 0.1 mg/ml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mg/ml, compound 2 showed a mean value of 0.003±0.004. At 0.08 mg/ml, the mean values were 0.006±0.018 for compound 2 and 0.013±0.013 for compound 4. At 0.1 mg/ml, compound 2 and compound 4 exhibited mean values of 0.014±0.017 and 0.014±0.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant.
Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.
Anahtar Kelimeler
Asteraceae, breast cancer, Cirsium italicum, flavonoid, histone deacetylase, reactive oxygen species
Kaynakça
- World Health Organization. Global cancer burden growing, amidst mounting need for services. https://www.who.int/news/item/01-02-2024-global-cancer-burden-growing--amidst-mounting-need-for-services (accessed 2025).
- World Health Organization. Breast cancer now most common form of cancer: WHO taking action. https://www.who.int/news/item/03-02-2021-breast-cancer-now-most-common-form-of-cancer-who-taking-action (accessed 2025).
- Crous-Bou M, Fung TT, Prescott J, Julin B, Du M, Sun Q et al. Mediterranean diet and telomere length in Nurses’ Health Study: population based cohort study. BMJ. 2014;349:6674.
- Schneider AS, Szanto AP. BRS Pathology Board Review Series. 6th edition, LWW. 2020.
- Cıkrıkcı S. Dioktilamino-3-hidroksiflavon temelli floresans probların sentezleri ve özelliklerinin incelenmesi [MSc Thesis] Istanbul: Istanbul Technical University Institute of Science. 2005.
- Singh AK, Bishayee A, Pandey AK. Targeting histone deacetylases with natural and synthetic agents: An emerging anticancer strategy. Nutrients. 2018;10:731.
- Allfrey VG, Faulkner R, Mirsky AE. Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc Natl Acad Sci USA. 1964;51:786-94.
- Gershey EL, Vidali G, Allfrey VG. Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone. J Biol Chem. 1968;243:5018-22.
- Basset SA, Barnett MP. The role of dietary histone deacetylases (HDACs) inhibitors in health and disease. Nutrients. 2014;6:4273-301.
- Bayram A, Igci M. Sirtuin genleri ve işlevleri. Fırat Tıp Dergisi. 2013;18:136-40.
