Kafa travması sonrası elde edilen beyin omurilik sıvısının sıçan kırık modelinde osteojenik potansiyeli

Yıl 2025, Cilt: 50 Sayı: 4, 1013 - 1024, 22.12.2025

Alaaddin Levent Özgözen , Nazlı Soygun İsmet Tan

https://doi.org/10.17826/cumj.1670756

Öz

Amaç: Travmatik beyin hasarı sonrası hızlanmış kırık iyileşmesi literatürde bildirilmiş olmasına rağmen, bu sürecin altında yatan mekanizmalar henüz netlik kazanmamıştır. Bu çalışmada, travmatik beyin hasarı sonrası sistemik dolaşıma katılan beyin omurilik sıvısının kırık iyileşmesini hızlandırabileceği hipotezini öne sürdük. Bu hipotezi test etmek amacıyla, travmatik beyin hasarı sonrası elde edilen beyin omurilik sıvısının sıçan modelinde kırık iyileşmesi üzerindeki etkilerini değerlendirmeyi amaçladık.
Gereç ve Yöntem: Çalışmaya toplam 39 erkek Wistar albino sıçan dahil edilerek üç gruba ayrıldı: kontrol grubu (n=15), deney grubu (n=14) ve kafa travması grubu (n=10). Kafa travması grubundaki sıçanlara deneysel kafa travması uygulandı ve bu sıçanlardan beyin omurilik sıvısı elde edildi. Kontrol ve deney gruplarında femur kırıkları oluşturularak intramedüller tespit uygulandı. Deney grubundaki sıçanların kırık bölgesine, kafa travması grubundan elde edilen beyin omurilik sıvısı enjekte edildi. Çalışmanın 30. gününde femurlar dezartiküle edildi ve kallus dokusu uzunluk ve genişlik açısından radyolojik olarak, histolojik olarak ise Huo derecelendirme sistemi kullanılarak değerlendirildi.
Bulgular: Deney grubunda kallus doku uzunluğu ortalama 11,96 ± 3,29 mm iken, kontrol grubunda 6,96 ± 3,30 mm olarak ölçülmüştür. Deney grubunun kallus doku uzunluğu kontrol grubuna kıyasla istatistiksel olarak anlamlı derecede daha yüksek bulunmuştur. Kallus doku kalınlığı ise deney grubunda 1,51 ± 0,60 mm, kontrol grubunda 1,17 ± 0,58 mm olarak saptanmış olup, iki grup arasında istatistiksel olarak anlamlı bir fark izlenmemiştir. Deney grubunun kallus maturasyon skoru (6,71 ± 0,73), kontrol grubuna (5,67 ± 1,05) kıyasla istatistiksel olarak anlamlı derecede daha yüksek bulunmuştur.
Sonuç: Travmatik beyin hasarı sonrası hızlanmış kırık iyileşmesinin mekanizması tam olarak aydınlatılamamıştır. Bu çalışmada, travmatik beyin hasarı sonrası elde edilen beyin omurilik sıvısının sıçan modelinde histolojik olarak anlamlı derecede kırık iyileşmesini hızlandırdığı ve kallus uzunluğunu artırdığı gösterilmiştir.

Anahtar Kelimeler

beyin omurilik sıvısı , kırık iyileşmesi , travmatik beyin hasarı

Proje Numarası

05042013

The osteogenic potential of cerebrospinal fluid obtained after traumatic brain injury in a rat fracture model

Öz

Purpose: Accelerated fracture healing following traumatic brain injury was reported in the literature, yet the underlying mechanisms remain unclear. This study hypothesizes that cerebrospinal fluid entering systemic circulation after traumatic brain injury contributes to the acceleration of fracture healing. To test this hypothesis, we aimed to evaluate the effects of cerebrospinal fluid collected post-traumatic brain injury on fracture healing in a rat model.
Materials and Methods: A total of 39 male Wistar albino rats were divided into three groups: control group (n=15), experimental group (n=14), and head trauma group (n=10). Head trauma was induced in the head trauma group, and cerebrospinal fluid was collected from the 10 rats following trauma. Femur fractures were induced and intramedullary fixation was performed in the control and experimental groups. Cerebrospinal fluid from the head trauma group was injected into the fracture site of the experimental group. On the 30th day, femurs were disarticulated, and callus tissue was evaluated radiologically for length and width and histologically using the Huo grading system.
Results: In the experimental group, the mean callus tissue length was 11.96 ± 3.29 mm, whereas in the control group it was 6.96 ± 3.30 mm. The callus tissue length in the experimental group was significantly higher compared to the control group. Callus tissue thickness was measured as 1.51 ± 0.60 mm in the experimental group and 1.17 ± 0.58 mm in the control group, with no statistically significant difference between the groups. The callus maturation score in the experimental group (6.71 ± 0.73) was significantly higher compared to the control group (5.67 ± 1.05).
Conclusion: The mechanism underlying accelerated fracture healing after traumatic brain injury remains elusive. In this study, cerebrospinal fluid collected post-traumatic brain injury demonstrated histologically significant acceleration of fracture healing and increased callus length.

Anahtar Kelimeler

cerebrospinal fluid , fracture healing , traumatic brain injury

Etik Beyan

Prior to the study, the necessary approvals were obtained from the Local Ethics Committee for Animal Experiments of Çukurova University, Republic of Turkey

Destekleyen Kurum

Çukurova University Faculty of Medicine

Proje Numarası

05042013

Teşekkür

We would like to express our gratitude to Prof. Dr. Gülfiliz Gönlüşen for her valuable assistance in the evaluation of bone preparations. We also thank Prof. Dr.Kenan Dağlıoğlu for his support during the animal experiments.

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