Investigation of MIR16-1 and MIR22 in chronic obstructive pulmonary disease
Öz
Purpose: Chronic obstructive pulmonary disease (COPD), characterized by respiratory tract inflammation, is a lung disease with increasing frequency with age and high morbidity and mortality. Small non-coding RNAs with a length of 19–25 nucleotides, known as microRNAs (miRNAs), control posttranscriptional levels of gene expression. Many dysregulated miRNAs have been demonstrated to be involved in various biological pathways like inflammation, cancer, airway epithelial differentiation, and lung development. Therefore, the aim of this study was to identify if MIR16-1 and MIR22 plasma expression levels might be utilized as a criterion for the diagnosis and monitoring of COPD.
Materials and Methods: This study contained a total of 58 male individuals, 33 COPD patients and 25 healthy controls. Real-time polymerase chain reaction (RT-PCR) was employed to examine the expression levels of MIR16-1-3p and MIR22-3p. Log2−delta delta CT (2-ΔΔCt) analysis was used to calculate miRNA expression data.
Results: No significant differences were discovered in the expression levels of MIR16-1-3p and MIR22-3p between healthy controls and COPD patients. When COPD patients were grouped as stable COPD and acute exacerbations of COPD, there was no change in the expression levels of MIR16-1-3p and MIR22-3p between different groups. Additionally, no correlation was found between the expression levels of either miRNA and the clinical features of COPD patients.
Conclusion: It was observed that there was no relationship between plasma MIR16-1-3p and MIR22-3p expression levels and COPD in this study. A deeper understanding of the connection between MIR16-1, MIR22, and COPD will be possible if the study is repeated with a wider sampling size.
Anahtar Kelimeler
Chronic obstructive pulmonary disease, MIR16-1, MIR22, RT-PCR
Destekleyen Kurum
None
Etik Beyan
Ethical approval was obtained from The Ethics Committee for Non-Interventional Scientific Research of Tokat Gaziosmanpasa University, Faculty of Medicine, with the registration number 25-MOBAEK-117 in its meeting dated 08.04.2025.
Kaynakça
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