Urtica dioica L. attenuates bisphenol A-induced hepatotoxicity in rats by suppressing apoptosis and inflammation
Öz
Purpose: The aim of this study was to investigate the protective effect of Urtica dioica L. (UD) against liver damage induced by bisphenol A (BPA) in rats. Materials and Methods: To this end, 48 healthy male Wistar albino rats were randomly divided into six groups: control (0.5 ml of normal saline); BPA (50 mg/kg); UD100 (100 mg/kg); UD200 (200 mg/kg); BPA+UD100; and BPA+UD200. All administrations were carried out for 56 days. The effects of the BPA and UD administrations on expression levels of Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2) and tumor necrosis factor-α (TNF-α) in liver tissue samples were evaluated using Western blotting and immunohistochemical analyses. Results: BPA caused significant increases in the Bax/Bcl-2 expression ratio (+45.2-fold) and TNF-α levels (+12.2-fold) in liver tissue. In groups treated with UD at 100 and 200 mg/kg doses, apoptosis was suppressed by upregulating Bcl-2 expression and downregulating Bax expression. Furthermore, the co-administration of UD was observed to significantly reduce the histopathological damage caused by BPA in liver tissue. In the BPA+UD100 group, inflammation was suppressed by downregulating TNF-α expression (-18.8-fold); however, in the BPA+UD200 group, TNF-α expression levels were higher than in the control group. Conclusion: These findings suggest that UD treatment can mitigate the adverse effects of BPA on liver tissue and enhance tissue healing in rats.
Anahtar Kelimeler
Bisphenol A, Urtica dioica L., hepatotoxicity, apoptosis, inflammation
Destekleyen Kurum
Etik Beyan
Kaynakça
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