Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells

Gulfidan Coskun; Dilek Şaker; Tuğçe Sapmaz Erçakallı; Özdem Karaoğlan; Sait Polat

doi:10.17826/cumj.1929465

EN TR

Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells

Öz

Purpose: This study aimed to investigate the effects of prednisolone and calcitriol on metabolic activity, steroidogenesis, and apoptosis-related markers in TM3 Leydig cells under human chorionic gonadotropin (hCG)-stimulated conditions. Materials and Methods: TM3 Leydig cells were divided into six groups: control, hCG, calcitriol, hCG + prednisolone, hCG + calcitriol, and hCG + prednisolone + calcitriol. CCK-8-based metabolic activity was evaluated using the CCK-8 assay. 3β-HSD and caspase-3 expression levels were examined immunocytochemically. Testosterone and INSL3 levels in the supernatant were measured by ELISA. Results: CCK-8-based metabolic activity increased in the hCG-treated group compared with the control group (116.88 ± 5.54% vs. 100.00 ± 6.80%). In contrast, metabolic activity decreased to 60.12 ± 5.53% in the calcitriol group, 45.02 ± 7.23% in the hCG + prednisolone group, 50.52 ± 2.97% in the hCG + calcitriol group, and 38.17 ± 3.54% in the triple-combination group. Total caspase-3-positive area was highest in the calcitriol-treated group (5.35 ± 1.38%). Testosterone levels were highest in the hCG + calcitriol group (34.39 ± 0.64 ng/mL). INSL3 levels increased from 41.02 ± 0.38 pg/mL in the control group to 50.28 ± 3.97 pg/mL in the calcitriol group, whereas they decreased to 35.57 ± 0.81 pg/mL in the hCG group and 35.06 ± 0.86 pg/mL in the triple-combination group. Conclusion: These findings suggest that the effects of calcitriol on Leydig cell responses may vary according to the conditions of administration, and that the suppressive effects of prednisolone may be modulated by accompanying stimuli.

Anahtar Kelimeler

TM3 Leydig cells, Calcitriol, Prednisolone, Human Chorionic Gonadotropin, Testosterone, INSL3, 3β-HSD, Apoptosis

TM3 leydig hücrelerinde kalsitriol ve prednizolonun steroidogenik fonksiyon üzerine etkileri

Öz

Amaç: Bu çalışmada, TM3 Leydig hücrelerinde hCG ile uyarılmış koşullarda prednizolon ve kalsitriolün metabolik aktivite, steroidogenez ve apopitozla ilişkili belirteçler üzerindeki etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada TM3 Leydig hücreleri; kontrol, hCG, kalsitriol, hCG + prednizolon, hCG + kalsitriol ve hCG + prednizolon + kalsitriol olmak üzere altı gruba ayrıldı. CCK-8 temelli metabolik aktivite CCK-8 yöntemiyle değerlendirildi. 3β-HSD ve Kaspaz-3 ekspresyonları immünositokimyasal olarak incelendi. Süpernatanda testosteron ve INSL3 düzeyleri ELISA ile ölçüldü. Bulgular: hCG uygulanan grupta CCK-8 temelli metabolik aktivite kontrol grubuna göre artış gösterdi (116.88 ± 5.54% vs. 100.00 ± 6.80%). Buna karşılık metabolik aktivite kalsitriol grubunda 60.12 ± 5.53%, hCG + prednizolon grubunda 45.02 ± 7.23%, hCG + kalsitriol grubunda 50.52 ± 2.97% ve üçlü kombinasyon grubunda 38.17 ± 3.54% olarak azaldı. Total kaspaz-3-pozitif alan en yüksek kalsitriol grubunda izlendi (5.35 ± 1.38%). Testosteron düzeyi en yüksek hCG + kalsitriol grubunda saptandı (34.39 ± 0.64 ng/mL). INSL3 düzeyi kontrol grubunda 41.02 ± 0.38 pg/mL iken, kalsitriol grubunda 50.28 ± 3.97 pg/mL’ye yükseldi; hCG ve üçlü kombinasyon gruplarında ise sırasıyla 35.57 ± 0.81 pg/mL ve 35.06 ± 0.86 pg/mL’ye düştü. Sonuç: Bulgular, kalsitriolün Leydig hücre yanıtları üzerindeki etkilerinin uygulama koşullarına göre değişebildiğini, prednizolonun baskılayıcı etkilerinin ise eşlik eden uyarılarla modüle edilebildiğini düşündürmektedir.

Anahtar Kelimeler

TM3 Leydig hücreleri, Kalsitriol, Prednizolon, İnsan Koryonik gonadotropin, Testosteron, INSL3, 3β-HSD, Apoptoz

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Histoloji ve Embriyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Gulfidan Coskun ^*
0000-0001-8219-2816
Türkiye

Dilek Şaker
0000-0002-5055-4226
Türkiye

Tuğçe Sapmaz Erçakallı
0000-0001-6927-3582
Türkiye

Özdem Karaoğlan
0000-0002-5117-6159
Türkiye

Sait Polat
0000-0003-1646-8831
Türkiye

Yayımlanma Tarihi

29 Haziran 2026

Gönderilme Tarihi

15 Nisan 2026

Kabul Tarihi

16 Haziran 2026

Yayımlandığı Sayı

Yıl 2026 Cilt: 51 Sayı: 2

DOI

https://doi.org/10.17826/cumj.1929465

IZ

https://izlik.org/JA34KF99YD

APA

Coskun, G., Şaker, D., Sapmaz Erçakallı, T., Karaoğlan, Ö., & Polat, S. (2026). Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells. Cukurova Medical Journal, 51(2), 586-595. https://doi.org/10.17826/cumj.1929465

AMA

1.Coskun G, Şaker D, Sapmaz Erçakallı T, Karaoğlan Ö, Polat S. Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells. Cukurova Med J. 2026;51(2):586-595. doi:10.17826/cumj.1929465

Chicago

Coskun, Gulfidan, Dilek Şaker, Tuğçe Sapmaz Erçakallı, Özdem Karaoğlan, ve Sait Polat. 2026. “Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells”. Cukurova Medical Journal 51 (2): 586-95. https://doi.org/10.17826/cumj.1929465.

EndNote

Coskun G, Şaker D, Sapmaz Erçakallı T, Karaoğlan Ö, Polat S (01 Haziran 2026) Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells. Cukurova Medical Journal 51 2 586–595.

IEEE

[1]G. Coskun, D. Şaker, T. Sapmaz Erçakallı, Ö. Karaoğlan, ve S. Polat, “Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells”, Cukurova Med J, c. 51, sy 2, ss. 586–595, Haz. 2026, doi: 10.17826/cumj.1929465.

ISNAD

Coskun, Gulfidan - Şaker, Dilek - Sapmaz Erçakallı, Tuğçe - Karaoğlan, Özdem - Polat, Sait. “Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells”. Cukurova Medical Journal 51/2 (01 Haziran 2026): 586-595. https://doi.org/10.17826/cumj.1929465.

JAMA

1.Coskun G, Şaker D, Sapmaz Erçakallı T, Karaoğlan Ö, Polat S. Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells. Cukurova Med J. 2026;51:586–595.

MLA

Coskun, Gulfidan, vd. “Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells”. Cukurova Medical Journal, c. 51, sy 2, Haziran 2026, ss. 586-95, doi:10.17826/cumj.1929465.

Vancouver

1.Gulfidan Coskun, Dilek Şaker, Tuğçe Sapmaz Erçakallı, Özdem Karaoğlan, Sait Polat. Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells. Cukurova Med J. 01 Haziran 2026;51(2):586-95. doi:10.17826/cumj.1929465

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Effects of calcitriol and prednisolone on steroidogenic function in TM3 leydig cells

Öz

Anahtar Kelimeler

TM3 leydig hücrelerinde kalsitriol ve prednizolonun steroidogenik fonksiyon üzerine etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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