Protective effects of Aronia melanocarpa seed oil against doxorubicin-induced cardiotoxicity in H9c2 cells: insights from apoptosis-related mRNA expression and molecular docking analyses
Öz
Purpose: This study aimed to investigate the protective effects of Aronia melanocarpa (chokeberry) seed oil (ASO) against doxorubicin (DOX)-induced cardiotoxicity in H9c2 cells by evaluating apoptosis-related mRNA expression and assessing the potential interactions of ASO-derived fatty acids with apoptosis-related proteins using molecular docking.
Materials and Methods: ASO cytotoxicity was assessed using the MTT assay, and concentrations of 10, 25, and 50 µg/mL were selected for further experiments based on the IC₅₀ value (377 µg/mL). H9c2 cells were pretreated with ASO for 30 min before exposure to 5 µM DOX for 24 h. Cell viability was assessed using the MTT assay, and the mRNA expression levels of caspase-3, Bax, and Bcl-2 were determined using RT-qPCR. Molecular docking analyses were performed to evaluate the binding affinities of ASO-derived fatty acids toward apoptosis-related proteins.
Results: DOX increased caspase-3 and Bax mRNA expression (~3.31-fold and ~1.62-fold vs. control, respectively) while decreasing Bcl-2 expression (~0.42-fold vs. control). ASO pretreatment dose-dependently attenuated these alterations, with the strongest effects observed at 50 µg/mL, reducing caspase-3 and Bax mRNA expression (~0.36-fold and ~0.64-fold vs. DOX, respectively) while increasing Bcl-2 expression (~2.14-fold vs. DOX). In addition, ASO increased the Bcl-2/Bax ratio compared with the DOX-treated group (~1.25-, ~2.62-, and ~3.31-fold vs. DOX at 10, 25, and 50 µg/mL, respectively). Molecular docking demonstrated potential interactions between ASO-derived fatty acids and apoptosis-related proteins, with binding energies ranging from −4.8 to −5.8 kcal/mol.
Conclusion: ASO attenuated DOX-induced apoptotic responses by modulating apoptosis-related mRNA expression, supporting its potential as a natural cardioprotective agent. Further in vivo studies are warranted to confirm these findings and clarify the underlying cardioprotective mechanisms.
Anahtar Kelimeler
Apoptosis, Aronia melanocarpa seed oil, cardioprotection, H9c2 cardiomyoblasts, doxorubicin, molecular docking
Destekleyen Kurum
N/A
Proje Numarası
N/A
Etik Beyan
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Teşekkür
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Kaynakça
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