Evaluation of epidermal growth factor receptor in odontogenic tumors and rare soft tissue tumors by immunohistochemical and fluorescence in situ hybridization methods

Kıvılcım Erdoğan; Mehmet Deveci; Gülfiliz Gönlüşen; Aysun Uğuz; Melek Ergin; Özge Dinigüzel; Serdar Özbarlas

doi:10.17826/cutf.209059

EN TR

Evaluation of epidermal growth factor receptor in odontogenic tumors and rare soft tissue tumors by immunohistochemical and fluorescence in situ hybridization methods

Abstract

Purpose: The aim of this study is to investigate the role of epidermal growth factor receptor (EGFR) in odontogenic tumors and rare soft tissue tumors.
Material and Methods: This study includes 28 cases (11 cases of odontogenic tumors and 17 cases of soft tissue tumors) which were diagnosed at Cukurova University Medical Faculty, Department of Pathology between 2002 and 2010. They were evaluated for immunohistochemical staining of EGFR and the presence of genetic abnormality by FISH method.
Results: Eleven cases were positively stained by immunohistochemistry. Eight cases had genetic abnormality of EGFR gene by FISH method. Nine of eleven odontogenic tumors (81,8%) showed overexpression of EGFR by immunohistochemical method or FISH method.
Conclusion: Our findings highlight that EGFR plays an important role in chordoma pathogenesis in soft tissue tumors. In addition to odontogenic tumors; two cases of vascular neoplasia, composite and retiform hemangioendothelioma, exhibited EGFR gene amplification. Interestingly, there is no avaliable data about EGFR gene status in these tumors. EGFR can be used for diagnostic purposes. Also, it is a target for anti-EGFR therapy. Therefore, EGFR gene status is a valuable marker for therapeutic management.

Keywords

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Yumuşak dokunun nadir görülen tümörlerinde ve odontojenik tümörlerde immünohistokimyasal yöntem ve floresan in situ hibridizasyonla epidermal growth faktör reseptör varlığının gösterilmesi

Öz

Amaç: Bu çalışmada amaç, çeşitli odontojenik tümörler ve nadir görülen yumuşak doku tümörlerinde epidermal growth faktör reseptörün (EGFR) rolünü ortaya koyabilmektir.
Gereç ve Yöntem: Bu çalışmada 2002-2010 yılları arasında Çukurova Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı’nda tanı almış onbir odontojenik tümör olgusu ve onyedi nadir görülen yumuşak doku tümörü olgusu olmak üzere toplam 28 olgu incelenmiştir. Bu olgularda immünohistokimyasal olarak EGFR boyanması ve FISH yöntemi ile genetik anormallik araştırılmıştır.
Bulgular: Çalışmamızda tüm olgulara immünohistokimyasal olarak uygulanan EGFR ile onbir olgu pozitif boyanmıştır. FISH yöntemi ile ise sekiz olguda genetik anormallik saptanmıştır. Odontojenik tümör olgularımızın dokuzunda (%81,8) immünohistokimyasal yöntemle ya da FISH metodu ile EGFR overekspresyonunun saptanmıştır.
Sonuç: Yumuşak doku tümörlerinde ise kordoma patogenezinde EGFR’nin önemi bulgularımızla tekrar vurgulanmış olup, daha önce EGFR varlığı gösterilmemiş olan odontojenik tümörler ile retiform ve kompozit hemanjioendotelyoma grubu tümörlerde de EGFR’nin rolü olduğu ileri sürülebilir. EGFR tanısal amaçlı kullanılabileceği gibi, anti-EGFR ilaçların hedefini teşkil ettiğinden, hastaların tedavi yönetiminde de oldukça değerlidir.

Anahtar Kelimeler

Odontojenik tümör, Yumuşak doku

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

-

Yazarlar

Kıvılcım Erdoğan Bu kişi benim

Mehmet Deveci Bu kişi benim

Gülfiliz Gönlüşen Bu kişi benim

Aysun Uğuz Bu kişi benim

Melek Ergin Bu kişi benim

Özge Dinigüzel Bu kişi benim

Serdar Özbarlas Bu kişi benim

Yayımlanma Tarihi

30 Haziran 2016

Gönderilme Tarihi

18 Ocak 2016

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2016 Cilt: 41 Sayı: 2

DOI

https://doi.org/10.17826/cutf.209059

IZ

https://izlik.org/JA38NP22LX

MLA

Erdoğan, Kıvılcım, vd. “Evaluation of epidermal growth factor receptor in odontogenic tumors and rare soft tissue tumors by immunohistochemical and fluorescence in situ hybridization methods”. Cukurova Medical Journal, c. 41, sy 2, Haziran 2016, ss. 316-25, doi:10.17826/cutf.209059.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Evaluation of epidermal growth factor receptor in odontogenic tumors and rare soft tissue tumors by immunohistochemical and fluorescence in situ hybridization methods

Abstract

Keywords

Yumuşak dokunun nadir görülen tümörlerinde ve odontojenik tümörlerde immünohistokimyasal yöntem ve floresan in situ hibridizasyonla epidermal growth faktör reseptör varlığının gösterilmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster