Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells

Ayşe Erdoğan; Aysun Özkan; Orhan Ünal; Canan Dülgeroğlu

doi:10.17826/cumj.340273

EN TR

Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells

Abstract

Purpose: In this study, we evaluated Sideritis stricta (S. stricta), as potential-oxidative agents against parental and epirubicin-HCl resistant H1299 cells.

Material and Methods: Oxidative stress biomarkers such as malondialdehyde level determined in cell lysates. Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after 20-600 μg/mL essential oil concentrations treated to parental and epirubicin-HCl (drug) resistant H1299 cells for 24, 48 and 72 h. Malondialdehyde levels were assayed for determining the membrane damaging effects.

Results: Parental H1299 cells were found to be more sensitive to cytotoxic effect of the essential oil. Essential oil showed cytotoxic and more selective effects depend on time and concentration. Essential oil caused increasing malondialdehyde level on both parental and drug resistant H1299 cells. The highest concentration of the essential oil (IC70) treatment caused the highest membrane damage on both parental and drug resistant H1299 cells.

Conclusions: Parental and epirubicin-HCl resistant H1299 cells showed different cellular response against potential antitumour and pro-oxidative effects of essential oil.

Keywords

Sideritis stricta,essential oil,membrane damage,parental and epirubicin- resistant H1299 cell line

Parental ve epirubicin-HCl dirençli H1299 hücrelerinde dağ çayı (Sideritis stricta Boiss & Heldr.) uçucu yağının sitotoksik ve membran hasar verici etkilerinin değerlendirilmesi

Öz

Amaç:. Bu çalışmada, Sideritis stricta (S. stricta) parental ve epirubicin-HCl dirençli H1299 hücrelerine karşı potansiyel oksidatif ajan olarak değerlendirilmiştir.

Gereç ve Yöntem: Hücre lisatlarında malondialdehit seviyesi gibi oksidatif stres biyobelirteçleri saptanmıştır. Hücre sitotoksisitesinin değerlendirilmesi, 20-600 μg/mL konsantrasyonlarında 24, 48 ve 72 saat parental ve epirubicin-HCl (ilaç) dirençli H1299 hücrelerine uçucu yağ uygulamasından sonra CellTiter-Blue® Hücre Canlılık Testi ve 3- (4,5-dimetil-2-tiazolil) -2,5-difenil-2H-tetrazolyumbromid (MTT) testi ile yapılmıştır. Malondialdehid seviyeleri membran hasar etkisini ortaya koymak için belirlenmiştir.

Bulgular: Parental H1299 hücreleri uçucu yağın sitotoksik etkisine karşı daha duyarlı bulunmuştur. Uçucu yağ, zaman ve konsantrasyona bağlı olarak sitotoksik ve daha seçici etkiler göstermiştir. Uçucu yağ, hem parental hem de ilaca dirençli H1299 hücrelerinde malondialdehit seviyesinde artışa neden olmuştur. Uçucu yağın (IC70) en yüksek konsantrasyonu, hem parental hem de ilaca dirençli H1299 hücrelerinde en yüksek membran hasarına neden olmuştur.

Sonuç: Parental ve epirubicin-HCl dirençli H1299 hücreleri uçucu yağın potansiyel antitümör ve pro-oksidatif etkilerine karşı farklı hücresel tepkiler göstermiştir.

Anahtar Kelimeler

Sideritis stricta,uçucu yağ,membran hasarı,parental ve epirubicin dirençli H1299 hücre dizisi

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Ayşe Erdoğan Bu kişi benim
Türkiye

Aysun Özkan
Türkiye

Orhan Ünal

Canan Dülgeroğlu

Yayımlanma Tarihi

30 Eylül 2018

Gönderilme Tarihi

28 Eylül 2017

Kabul Tarihi

8 Aralık 2017

Yayımlandığı Sayı

Yıl 2018 Cilt: 43 Sayı: 3

DOI

https://doi.org/10.17826/cumj.340273

IZ

https://izlik.org/JA53JK33HT

MLA

Erdoğan, Ayşe, vd. “Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells”. Cukurova Medical Journal, c. 43, sy 3, Eylül 2018, ss. 669-77, doi:10.17826/cumj.340273.