Dipiron farelerde anksiyolitik-benzeri etkiler oluşturuyor mu?

Ruhan Deniz Topuz; Özgür Gündüz; Dikmen Dökmeci; Çetin Hakan Karadağ; Ahmet Ulugöl

doi:10.17826/cumj.488406

TR EN

Dipiron farelerde anksiyolitik-benzeri etkiler oluşturuyor mu?

Öz

Amaç: Yakın zamanda parasetamol’ün anksiyolitik-benzeri etkileri olduğu ve bu etkilerine kannabinoid CB1 reseptörlerinin aracılık ettiği gösterilmiştir. Etkilerinde santral sinir sisteminin rolü olduğu uzun zamandır düşünülen ve etkileri açısından non-steroid anti-inflamatuvar ilaçlardan çok parasetamol ile benzerlik gösteren bir analjezik olan dipiron’un da antinosiseptif etkisinde kannabinoid sistemin rolü olduğu belirtilmiştir. Bu verilerden yola çıkarak dipiron’un anksiyolitik etki gösterebileceği düşünüldü; varsa bu etkisinde kannabinoid ve TRPV1 reseptörlerinin katkısının araştırılması planlandı.

Gereç ve Yöntem: Balb-c farelerde dipiron (150, 300, 600 mg/kg, i.p.) verildikten sonra üç odalı sosyallik ve sosyal yenilik testi, açık alan testi ve yükseltilmiş artı labirent testleri uygulanması, etki görülürse kannabinoid CB1 antagonisti AM251 (1 mg/kg i.p.), CB2 antagonisti SR 144528 (1 mg/kg i.p.) ve TRPV1 antagonisti kapsazepin (3 mg/kg i.p.) ile birlikte verilmesi düşünüldü.

Bulgular: Dipiron herhangi bir dozunda davranış testlerinin (üç odalı sosyallik ve sosyal yenilik testi, açık alan testi ve yükseltilmiş artı labirent testi) hiçbirisinde etki göstermedi. Bu nedenle, dipiron ile birlikte kannabinoid CB1, CB2 ve TRPV1 reseptör antagonistlerinin birlikte uygulanacağı aşamaya geçilmedi.

Sonuç: Parasetamol’den farklı olarak, dipiron farelerde anksiyolitik-benzeri etkilere yol açmamaktadır. Deneysel modeller ve ölçüm yöntemlerindeki farklılıklar bu bulgularımızın nedeni olabilir.

Anahtar Kelimeler

dipiron,anksiyete,kannabinoid

Does dipyrone produce anxiolytic-like effects in mice?

Abstract

Purpose: Paracetamol has been shown to exert anxiolytic-like effects mediated by endocannabinoids via cannabinoid CB1 receptors. Dipyrone is an analgesic with similar effects to paracetamol rather than non-steroidal anti-inflammatory drugs. Involvement of central structures to its effects are long under debate, whereas recent findings suggesting contribution of cannabinoid CB1 receptors to its antinociceptive effect support this argument. Taken together, the purpose of this study was to investigate whether dipyrone possesses anxiolytic-like behavior; contribution of cannabinoid CB1 and CB2 receptors and TRPV1 receptors will be determined in case of observing any effect of dipyrone in anxiety tests.

Material and Methods: Balb-c mice effects of dipyrone (150, 300, 600 mg/kg, i.p.) were assessed in three-chamber social interaction, open-field, elevated plus-maze and rota rod tests. The cannabinoid CB1 antagonist AM251 (1 mg/kg i.p.), the CB2 antagonist SR 144528 (1 mg/kg i.p.) and the TRPV1 antagonist capsazepine (3 mg/kg i.p.) were going to be administered before dipyrone injections if any effect of dipyrone occurs.

Results: Dipyrone had no effect at any dose in behavioral tests (three-chamber social interaction, open-field, elevated plus-maze and rota rod tests). Therefore, dipyrone is not tested together with the cannabinoid CB1 and CB2 antagonists and the TRPV1 receptor antagonist.

Conclusion: Unlike paracetamol, dipyrone did not possess anxiolytic-like effects in mice. Discrepancies in experimental models and methodologies may be the reason of our results.

Keywords

dipyrone,anxiety,cannabinoid

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Ruhan Deniz Topuz ^*
0000-0002-1520-3754
Türkiye

Özgür Gündüz Bu kişi benim
0000-0002-2470-3021

Dikmen Dökmeci Bu kişi benim
0000-0002-7685-127X

Çetin Hakan Karadağ Bu kişi benim
0000-0002-4763-986X

Ahmet Ulugöl
0000-0003-4643-1124

Yayımlanma Tarihi

30 Eylül 2019

Gönderilme Tarihi

27 Kasım 2018

Kabul Tarihi

13 Ocak 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 44 Sayı: 3

DOI

https://doi.org/10.17826/cumj.488406

IZ

https://izlik.org/JA45RP74FX

MLA

Topuz, Ruhan Deniz, vd. “Dipiron farelerde anksiyolitik-benzeri etkiler oluşturuyor mu?”. Cukurova Medical Journal, c. 44, sy 3, Eylül 2019, ss. 866-74, doi:10.17826/cumj.488406.