Rhodamine B Triggers Ovarian Toxicity Through Oxidative Stress

Syiska Atik Maryanti; Siti Suciati; Endang Sri Wahyuni; Sanarto Santoso; İ Wayan Arsana Wiyasa

doi:10.17826/cutf.17415

EN TR

Rhodamine B Triggers Ovarian Toxicity Through Oxidative Stress

Abstract

Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05). The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05). Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium.

Keywords

Dye , toxicity , ovarium , endometrium , oxidative stress

Rodamin B, Oksidatif Stress Aracılığı ile Ovarian Toksisitesi

Öz

Amaç: Çalışmanın amacı, rodamin B"ye maruziyetin, ovarian oksidatif stress, ovarian folikül, 17β-Estradiol hormonu ve endomerium kalınlığı üzerindeki etkisini araştırmak. Materyal Metod: Toplam 28 dişi sıçan kontrol grubu ile birlikte 4 gruba ayrılmıştır. Gruplar 4.5, 9 ve 18 miligram/200 gram vücut ağırlığı olacak şekilde rodamin B ile muamele edilmişir.Rodamin B, 36 gün boyunca probla birlikte oral olarak uygulanmıştır. MDA seviyelerinin analizi spektrofotometrik olarak yapılmıştır. Ovarian folikül sayısı ve endometrium kalınlığı analizleri histopatolojik olarak hematoksilin eozin boyama ile yapılmıştır. 17β-Estradiol seviyesi ELISA ile ölçülmüştür. Bulgular: Dişi sıçanlara farklı dozlarda Rodamin B uygulanması, ovarian MDA seviyelerini kontrollere gore anlamlı şekilde arttırmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozları primer, sekonder ve De Graaf folikül sayısını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozlarının uygulanması 17β-estradiol seviyesini kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Rodamin B uygulanması endometrium kalınlığını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Sonuç: Rodamin B uygulamasının, oksidatif stress aracılığı ile ovarian toksisitesini tetiklediği, folikül sayısını azalttığı ve, 17β-Estradiol seviyesini azaltalarak endometriyum kalınlığını düşürdüğü sonucuna varılmıştır.

Anahtar Kelimeler

Boya , Toksisite , ovarian , endometriyum , oksidatif stres

Kaynakça

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Yazışma Adresi / Address for Correspondence: Dr. Syiska Atik Maryanti Brawijaya University Faculty of Medicine Midwifery Master Study Programme, Jl. Veteran, Malang, East Java, INDONESIA Email: syiska_hermawan@yahoo.com G eliş tarihi/Received on: 30.01.2014
Kabul tarihi/Accepted on:28.02.2014

Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Syiska Atik Maryanti Bu kişi benim

Siti Suciati Bu kişi benim

Endang Sri Wahyuni Bu kişi benim

Sanarto Santoso Bu kişi benim

İ Wayan Arsana Wiyasa Bu kişi benim

Yayımlanma Tarihi

22 Temmuz 2014

Gönderilme Tarihi

22 Temmuz 2014

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 1970 Cilt: 39 Sayı: 3

DOI

https://doi.org/10.17826/cutf.17415

IZ

https://izlik.org/JA57ZR76FW

MLA

Maryanti, Syiska Atik, vd. “Rhodamine B Triggers Ovarian Toxicity Through Oxidative Stress”. Cukurova Medical Journal, c. 39, sy 3, Eylül 2014, ss. 451-7, doi:10.17826/cutf.17415.