Investigation of benfotiamine’s protective effects on liver tissue in experimental carbon tetrachloride induced liver injury

Bülent Karakaya; Tuncay Kuloğlu; Erhan Önalan; Nalan Kaya Tektemur; Emir Dönder

doi:10.17826/cumj.676060

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Karbon tetraklorür ile oluşturulan deneysel karaciğer hasarında benfotiamin’in karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi

Öz

Amaç: Bu çalışmada karbon tetraklorür (CCl4) ile oluşturulan deneysel karaciğer hasarında benfotiaminin karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi amaçlanmıştır.
Gereç ve Yöntem: Çalışmada, 30 adet Wistar albino cinsi erkek sıçanlar kullanıldı. Deney hayvanları 5 eşit gruba ayrıldı. Kontrol grubuna 14 günlük deney süresince herhangi bir uygulama yapılmadı. CCl4 grubuna ise 1. ve 8. günlerde 1ml/kg CCl4: zeytinyağı (1:2) intraperitoneal (i.p) verildi. CCl4 +benfotiamin grubuna 1. ve 8. günlerde iki defa 1 ml/kg CCl4: zeytinyağı (1:2) karışımı ve 70 mg/kg/gün oral benfotiamin verildi. Benfotiamin grubuna 70 mg/kg/gün oral benfotiamin verildi. Zeytinyağı grubuna 1. ve 8. günlerde 2 ml/kg zeytinyağı i.p. olarak verildi. Deney sonunda sıçanlar anestezi altında dekapite edildi. Karaciğer dokuları çıkarılıp incelemeler için parafin bloklar hazırlandı. Ayrıca malondialdehid (MDA) çalışması için dokular – 80 0 C de saklandı.
Bulgular: Kontrol, benfotiamin ve zeytinyağı grupları arasında bakılan parametrelerde anlamlı bir farklılık yoktu. Kontrol grubu ile karşılaştırıldığında CCl4 grubunda, MDA, apoptozis ve bax immünreaktivitesinde anlamlı artış izlendi. CCl4 grubu ile karşılaştırıldığında CCl4 + benfotiamin grubunda ise MDA, apoptozis ve bax immünreaktivitesinde anlamlı azalma görüldü.
Sonuç: CCl4’ün MDA, apoptozis ve bax immünreaktivitesini arttırdığı, tedavi olarak verilen benfotiaminin bu parametreleri azalttığı görüldü.

Anahtar Kelimeler

Sıçan,karbon tetraklorür,benfotiamin

Investigation of benfotiamine’s protective effects on liver tissue in experimental carbon tetrachloride induced liver injury

Abstract

Purpose: In this study, we aimed to investigate the protective effects of benfotiamine on experimental liver injury caused by carbon tetrachloride (CCl4).
Materials and Methods: In this study, 30 male Wistar albino rats were used. Rats were equally divided into 5 groups. No application was made to control group. The CCl4 group was injected i.p with1ml/kg CCl4:olive oil (1:2) mixture on the 1st and 8th days, and the CCl4+benfotiamine group was treated i.p with 1 ml/kg CCl4: olive oil (1:2) mixture twice on the 1st and 8th days and orally with 70 mg/kg/day benfotiamine. To the benfotiamine group, 70 mg/kg/day benfotiamine was given orally for 14 days. To the olive oil group, 2 ml/kg olive oil was given i.p. on 1st and 8th days. Finally, rats were decapitated. Liver tissues were removed and paraffin blocks were prepared. Tissues were stored at –80 oC for malonaldeyhde (MDA) assay.
Results: There were no significant differences between the control, benfotiamine and olive oil groups. Compared with the control group, there was a significant increase in MDA, apoptosis and bax immunoreactivity in CCl4 group. Compared with the CCl4 group, there was a significant decrease in MDA, apoptosis and bax immunoreactivity in the CCl4+benfotiamine group.
Conclusion: CCl4 increases MDA, apoptosis and bax immunoreactivity, and benfotiamine, given as treatment, reduces these parameters.

Keywords

Rat,carbon tetrachloride,benfotiamine

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Bülent Karakaya
0000-0001-8643-0017
Türkiye

Tuncay Kuloğlu
0000-0001-9874-3838
Türkiye

Erhan Önalan ^*
0000-0001-5395-0390
Türkiye

Nalan Kaya Tektemur
0000-0001-8880-4932
Türkiye

Emir Dönder
0000-0003-2537-6023
Türkiye

Yayımlanma Tarihi

30 Haziran 2020

Gönderilme Tarihi

16 Ocak 2020

Kabul Tarihi

13 Nisan 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 45 Sayı: 2

DOI

https://doi.org/10.17826/cumj.676060

IZ

https://izlik.org/JA75BA94ZW

MLA

Karakaya, Bülent, vd. “Investigation of benfotiamine’s protective effects on liver tissue in experimental carbon tetrachloride induced liver injury”. Cukurova Medical Journal, c. 45, sy 2, Haziran 2020, ss. 680-7, doi:10.17826/cumj.676060.

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Karbon tetraklorür ile oluşturulan deneysel karaciğer hasarında benfotiamin’in karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi

Öz

Anahtar Kelimeler

Investigation of benfotiamine’s protective effects on liver tissue in experimental carbon tetrachloride induced liver injury

Abstract

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Kaynakça

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Konular

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