Investigation of uroprotective effects of seed methanol extracts of Hypericum triquetrifolium Turra. on cyclophosphamide-induced bladder hemorrhagic cystitis and nephrotoxicity in Wistar albino rats
Öz
Purpose: This study investigated the possible uroprotective effects of Hypericum triquetrifolium Turra. (HT) seed methanol extracts (25,50,100 mg/kg, i.p., for 6 days) against cyclophosphamide (CYP)-induced (150 mg/kg, single dose, i.p.) acute bladder hemorrhagic cystitis (HC) and nephrotoxicity in rats.
Materials and Methods: Wistar albino rats used in this study were divided into nine groups, each including seven rats. Group 1 (control) was treated with 0.5ml saline (SF) and Group 2 was treated with CYP (150 mg/kg). Groups 3, 4, 5 were treated with 25, 50, 100 mg/kg HT, respectively while groups 6, 7, 8 were treated with 25, 50, 100 mg/kg CYP + HT, respectively. Finally, Group 9 (control-2) was treated with 0.5ml-%0.2 dimethyl sulfoxide (DMSO). The serum creatinine, blood urea nitrogen (BUN), superoxide dismutase (SOD) and catalase (CAT) levels were measured in blood serum.
Results: The CYP-treated rats histopathologically had mild-moderate bladder and renal injuries. The serum creatinine and BUN levels, which are the biochemical markers of renal injury, significantly increased compared to the control group.
Conclusion: HT showed a protective effect on CYP-related bladder HC and nephrotoxicity in rats by inhibiting inflammation and apoptosis.
Anahtar Kelimeler
Destekleyen Kurum
This study was supported by Mardin Artuklu University - Coordination Unit of Scientific Research Project (MAU-BAP-15-SHMYO-23).
Proje Numarası
MAU-BAP-15-SHMYO-23
Kaynakça
- 1. Jurado-Garcia JM, Sanchez A, Pajaras B. Combined oral cyclophosphamide and bevacizumab in heavily pretreated ovarian cancer. Clin Transl Oncol. 2008;10(9):583-86.
- 2. Caglar K, Kinalp C, Arpaci F, Turan M, Saglam K, Ozturk B et al. Cumulative prior dose of cisplatin as a cause of the nephrotoxicity of high-dose chemotherapy followed by autologous stem-cell transplantation. Nephrol Dial Transplant. 2002;17(11):1931-35.
- 3. Abraham P, Rabi S. Nitrosative stress, protein tyrosine nitration, PARP activation and NAD depletion in the kidneys of rats after single dose of cyclophosphamide. Clin Exp Nephrol. 2009;13(4):281–87.
- 4. Chuang YC, Yoshimura N, Huang CC, Wu M, Tyagi P, Chancellor MB. Expression of E-series prostaglandin (EP) receptors and urodynamic effects of an EP4 receptor antagonist on cyclophosphamide-induced overactive bladder in rats. BJU Int. 2010;106(11):1782-87.
- 5. Korkmaz A, Topal T, Oter S. Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis. Cell Biol Toxicol. 2007;23(5):303-12.
- 6. Liu Q, Lin X, Li H, Yuan J, Peng Y, Dong L, Dai S. Paeoniflorin ameliorates renal function in cyclophosphamide-induced mice via AMPK suppressed inflammation and apoptosis. Biomed Pharmacother. 2016a;84:1899-905.
- 7. Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB. The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats. Urology. 2012;80(6):1392–98.
- 8. Topal T, Oztas Y, Korkmaz A, Sadir S, Oter S, Omer C, Bilgic H. Melatonin ameliorates bladder damage induced by cyclophosphamide in rats. J Pineal Res. 2005;38(4):272-77.
- 9. Bhatia K, Kaur M, Atif F, Ali M, Rehman H, Rahman S, Raisuddin S. Aqueous extract of Trigonella foenum-graecum L. ameliorates additive urotoxicity of buthionine sulfoximine and cyclophosphamide in mice. Food Chem Toxicol. 2006;44(10):1744-50.
- 10. Tripathi DN, Jena GB. Effect of melatonin on the expression of Nrf2 and NF-kappaB during cyclophosphamide-induced urinary bladder injury in rat. J Pineal Res. 2010;48(4):324–31.
