Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri

Sevil Arabacı Tamer; Alper Yıldırım; Ozge Cevik; Burak Aksu; Meral Yüksel; Ekin Kuntsal; Serap Sirvancı; Berrak Yeğen

EN TR

Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri

Öz

maç: Bu çalışmda melatonin ön-tedavisinin, sıçanlarda mide ülserine bağlı gerçekleşen mikrobiyota değişimine ve oksidatif hasara karşı koruyucu etkilerini araştırmak hedeflenmiştir. Gereç ve Yöntem: Wistar albino sıçanlara (n=32) 12 gün boyunca içme suyu, melatonin (4mg/kg/gün), antibiyotik karışımı (AB; 1 g/L ampisilin + 1 g/L neomisin+ 1 g/L metronidazol), melatonin+ AB içme suyunda verildi. Ardından ülser oluşturuldu. Katkısız içme suyu alan sıçanların yarısına cerrahi işlem yapıldı ama asetik asit uygulanmadı (kontrol grubu; n=8). Cerrahi sonrasındaki 3 gün boyunca uygulanan tüm tedavilere devam edildi. Mide dokuları biyokimyasal ve histopatolojik incelemeler için çıkarıldı. Rektumdan alınan fekal örnekler bakteriyolojik ölçümler için uygun koşullarda saklandı. Bulgular: Tedavi almayan ülser grubunun mide dokusunda MDA, MPO, 8-OHdG, luminol ve lusigenin kemilüminesans seviyeleri, TNF-α ve IL-8 protein ekspresyonları kontrol grubuna göre anlamlı derecede artarken, antioksidan GSH seviyesinin azaldığı gözlendi. Melatonin+AB ön-tedavi uygulaması antioksidan GSH seviyesini ve anti-inflamatuvar IL-10 miktarını artırmış, kaspaz-3 aktivitesini baskılamış ve dokuya nötrofil göçünü kontrol düzeyine inecek şekilde azaltmıştır. Sonuç: Etkin bir antioksidan ve radikal süpürücü olan melatoninin antibiyotik ile birlikte verilmesinin, ülser iyileşmesini hızlandırmada ve kolondaki dağılımı bozulan bakteriyel çeşitliliği düzeltmede daha etkili olacağı öngörülmektedir.

Anahtar Kelimeler

Mide ülseri , asetik asit , melatonin , mikrobiyota , inflamasyon , oksidatif stres

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Tıbbi Fizyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Sevil Arabacı Tamer ^*
0000-0002-8701-6894
Türkiye

Alper Yıldırım Bu kişi benim
0000-0002-3328-1692
Türkiye

Ozge Cevik
0000-0002-9325-3757
Türkiye

Burak Aksu Bu kişi benim
0000-0002-3439-9158
Türkiye

Meral Yüksel
0000-0002-4760-3306
Türkiye

Ekin Kuntsal Bu kişi benim
0000-0002-1330-9221
Türkiye

Serap Sirvancı
0000-0001-7683-4587
Türkiye

Berrak Yeğen
0000-0003-0791-0165
Türkiye

Yayımlanma Tarihi

31 Mart 2021

Gönderilme Tarihi

14 Ağustos 2020

Kabul Tarihi

20 Ekim 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 46 Sayı: 1

IZ

https://izlik.org/JA35PN76GU

MLA

Arabacı Tamer, Sevil, vd. “Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri”. Cukurova Medical Journal, c. 46, sy 1, Mart 2021, ss. 186-00, https://izlik.org/JA35PN76GU.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri

The ameliorative effects of melatonin on acetic acid-induced gastric ulcer in rats via its modulatory effects on gut microbiota

Abstract

Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

IZ

Kaynak Göster