IRF5 inhibits prostate cancer metastasis and drug resistance by decreasing CXCR4/CXCL12 complex

Burcin Abas; Nesrin Büyükkarıncalı; Ozge Cevik

TR EN

IRF5, CXCR4/CXCL12 kompleksini azaltarak prostat kanseri metastazını ve ilaç direncini inhibe eder

Öz

Amaç: Prostat kanseri erkeklerde en sık görülen kanser türüdür ve tedavisinde ilaç direnci tipiktir. Kemokinler özellikle tümör büyümesinde ve ilaç direnç mekanizmalarında rol oynarlar. IRF5, immün yanıtta kritik bir transkripsiyon faktörüdür ve bu çalışmada kemokin ailesi CXCR4 ve CXCL12 ile ilişkisi araştırılmıştır. Gereç ve Yöntem: pIRF5 plazmidi, androjenden bağımsız bir insan prostat kanseri hücre hattında (PC3) transfekte edildi ve IRF5 proteini aşırı eksprese edildi. CXCR4 ve CXCL12 protein ekspresyon seviyeleri western blot ve gen ekspresyon seviyeleri ile qPCR yöntemi ile belirlendi. Ek olarak, IRF5 transfeksiyonundan sonra hücrelerde koloni oluşumu incelendi ve hücre ortamında CXCL12 sekresyonu ölçüldü. Bulgular: IRF5 ile transfekte edilmiş PC3 hücrelerinde hücre canlılığı ve koloni oluşumunun önemli ölçüde azaldığı bulundu. Ek olarak, IRF5 ile transfekte edilmiş hücrelerin CXCR4 ve CXCL12 protein ekspresyonu ve gen ekspresyon seviyelerinin önemli ölçüde azaldığı tespit edildi. Sonuç: Bu çalışma, bir transkripsiyon faktörü olan IRF5'in prostat kanserinde gelişen mikroçevre aracılı metastazda yer alan CXCR4/CXCL12'yi etkilediğini göstermektedir. Böylece prostat kanseri tedavisinde IRF5 gen tedavisi metastazı önleyebilir ve bu konuda yeni geliştirilen tedavi yöntemlerine önemli katkılar sunabilir.

Anahtar Kelimeler

IRF5 , Prostat kanseri , CXCR4 , CXCL12 , kemokinler

IRF5 inhibits prostate cancer metastasis and drug resistance by decreasing CXCR4/CXCL12 complex

Abstract

Purpose: Prostate cancer is the most common type of cancer in men, and drug resistance is typical in its treatment. Chemokines especially play a role in tumor growth and drug resistance mechanisms. IRF5 is a critical transcription factor in immune response, and its relationship with the chemokine family CXCR4 and CXCL12 was investigated in this study. Materials and Methods: The pIRF5 plasmid was transfected in an androgen-independent human prostate cancer cell line (PC3), and the IRF5 protein was overexpressed. CXCR4 and CXCL12 protein expression levels were determined by western blot and gene expression levels by the qPCR method. In addition, colony formation was examined in cells after IRF5 transfection, and CXCL12 secretion was measured in cell media. Results: Cell viability and colony formation were found to be significantly reduced in IRF5 transfected PC3 cells. In addition, CXCR4 and CXCL12 protein expression and gene expression levels of IRF5 transfected cells were found to be significantly decreased. Conclusion: This study shows that IRF5, a transcription factor, affects CXCR4/CXCL12, which is involved in microenvironment-mediated metastasis developing in prostate cancer. Thus, in the treatment of prostate cancer, IRF5 gene therapy can prevent metastasis and offer essential contributions to newly developed treatment methods in this regard.

Keywords

IRF5 , Prostate cancer , CXCR4 , CXCL12 , chemokines

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Burcin Abas Bu kişi benim
0000-0002-1018-5577
Türkiye

Nesrin Büyükkarıncalı Bu kişi benim
0000-0003-0422-1225
Türkiye

Ozge Cevik ^*
0000-0002-9325-3757
Türkiye

Yayımlanma Tarihi

30 Aralık 2021

Gönderilme Tarihi

16 Ağustos 2021

Kabul Tarihi

19 Ekim 2021

Yayımlandığı Sayı

Yıl 1970 Cilt: 46 Sayı: 4

IZ

https://izlik.org/JA98SX76WL

MLA

Abas, Burcin, vd. “IRF5 inhibits prostate cancer metastasis and drug resistance by decreasing CXCR4/CXCL12 complex”. Cukurova Medical Journal, c. 46, sy 4, Aralık 2021, ss. 1632-9, https://izlik.org/JA98SX76WL.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

IRF5, CXCR4/CXCL12 kompleksini azaltarak prostat kanseri metastazını ve ilaç direncini inhibe eder

Öz

Anahtar Kelimeler

IRF5 inhibits prostate cancer metastasis and drug resistance by decreasing CXCR4/CXCL12 complex

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

IZ

Kaynak Göster