Protective effect of daidzein on ovarian ischemia‑reperfusion injury in rats

Erdem Toktay; Muhammet Ali Gürbüz; Tuğba Bal; Özlem Özgül; Elif Erbaş; Rüstem Anıl Ugan; Jale Selli

doi:10.17826/cumj.993250

TR EN

Daidzeinin ovaryum iskemi reperfüzyonu hasarındaki koruyucu etkisi

Öz

Amaç: Bu çalışmada, güçlü antioksidan daidzeinin (DZ) over iskemi ve reperfüzyon hasarı üzerindeki potansiyel etkisini araştırdık. Gereç ve Yöntem: Toplam 42 adet dişi Sprague-Dawley sıçan rastgele yedi gruba ayrıldı. Deney modeli için, 3saatlik iskeminin ardından klempler çıkarıldı ve tekrar kan akışı sağlandı. Reperfüzyon 3 saatin sonunda sonlandırıldı. Daidzein, iskemi (I) ve iskemi ve reperfüzyon (I/R) işlemlerinden 30 dakika önce hayvanlara ağızdan 35 ve 70 mg/kg dozlarında uygulandı. Bulgular: I ve I/R gruplarında IL-1β, IL 6 ve Caspase 3'ün şiddetli immünoreaktivite görüldü. I+DZ35 ve I/R+DZ35 gruplarında IL-1β, IL 6 ve Kaspaz 3'ün immünreaktivitesi orta düzeyde iken, I+DZ70 ve I/R+DZ70 gruplarında hafif pozitiftir. Daidzein ile tedavi edilen gruplarda SOD aktivite seviyesi artarken, MDA seviyeleri azaldı. Ayrıca I/R + DZ70 ve IR+DZ35 gruplarında I/R grubuna göre doza bağlı olarak kanama alanları ve inflamatuar hücre göçü azaldı. Sonuç: Daidzein, over iskemi-reperfüzyon hasarının tedavisinde güçlü bir koruyucu role sahiptir ve terapötik bir ajan olarak kullanılabilir.

Anahtar Kelimeler

Daidzein , ovaryum , iskemisi ve reperfüzyon hasarı , sıçan

Protective effect of daidzein on ovarian ischemia‑reperfusion injury in rats

Abstract

Purpose: In this study, our aim was to investigate the potential effects of strong antioxidant daidzein (DZ) on ovarian ischemia and reperfusion injury. Materials and Methods: A total of 42 female Sprague-Dawley rats were randomly divided into seven groups. For the experimental model, the clamps were removed after 3 hours of ischemia, and blood flow was provided again. Then, reperfusion process was terminated for 3 hours. Daidzein was orally administered to animals at doses of 35 and 70 mg/kg 30 minutes before ischemia (I) and ischemia and reperfusion (I/R) procedures. Results: Severe immunoreactivity of the IL-1β, IL-6 and Caspase-3 were detected in I and I/R groups. Moderate immunoreactivity of IL-1β, IL-6 and Caspase-3 was detected in I+DZ35 and I/R+DZ35 groups, and slightly positivity was detected in I+DZ70 and I/R+DZ70 groups. The SOD activity level increased in the groups treated with Daidzein, while MDA levels decreased. In addition, hemorrhage areas and inflammatory cell migration decreased in I/R+DZ70 and I/R+DZ35 groups, when compared to I/R group in a dose dependent manner. Conclusion: Daidzein has a strong protective role in the treatment of ovarian ischemia-reperfusion injury and can be used as a therapeutic agent.

Keywords

Daidzein , ovary , ischemia‑reperfusion injury , rat

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Erdem Toktay ^*
0000-0002-7447-6023
Türkiye

Muhammet Ali Gürbüz
0000-0001-6628-3363
Türkiye

Tuğba Bal
0000-0001-8257-8639
Türkiye

Özlem Özgül
0000-0001-9852-8839
Türkiye

Elif Erbaş
0000-0003-1750-3889
Türkiye

Rüstem Anıl Ugan
0000-0002-4837-2343
Türkiye

Jale Selli
0000-0002-1750-1130
Türkiye

Yayımlanma Tarihi

31 Mart 2022

Gönderilme Tarihi

14 Eylül 2021

Kabul Tarihi

4 Ocak 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 47 Sayı: 1

DOI

https://doi.org/10.17826/cumj.993250

IZ

https://izlik.org/JA66GY94UF

MLA

Toktay, Erdem, vd. “Protective effect of daidzein on ovarian ischemia‑reperfusion injury in rats”. Cukurova Medical Journal, c. 47, sy 1, Mart 2022, ss. 102-10, doi:10.17826/cumj.993250.