Ovarian Autografting In Rabbits: Hormonal And Morphological Evaluations.

Yıl 2013, Cilt: 38 Sayı: 3, 403 - 411, 01.09.2013

M. Turan Çetin İbrahim Ürünsak Abdulhakim Arısoy Cihan Çetin Taner Kafadar İlhan Tuncer

Öz

Purpose: The functional and histological changes in the ovarian tissue of 10 female mature rabbits were evaluated before and after ovarian autografting. Material and Methods: Preoperative (one month before the autograft) and postoperative (two months after the autograft) estradiol ( E2) and progesterone ( P) levels were measured, and histological evaluations were performed by light microscope. One month later, ovarian autografting was performed on the rabbits. Results: Ovarian tissue was observed in 80% of the transplanted ovarian samples. Both preoperative and postoperative P values increased from the basal to the late period and the differences were statistically significant. There was no significant difference between preoperative and postoperative E2 values from the basal to the late period. Preoperative P values were statistically significantly higher than postoperative P values in all three time periods. Preoperative E2 values were also higher than postoperative E2 values, but the differences were not statistically significant. Conclusion: If autografting is performed by employing microsurgical techniques, ovarian autografts will have similar postoperative hormonal function and morphology as in preoperative period.

Anahtar Kelimeler

ovarian autograft, ovarian hormonal function, ovarian morphology.

Tavşanlarda Overyen Otograft: Hormonal ve Morfolojik Değerlendirme

Öz

Giriş: 10 adet erişkin dişi tavşanda otograft öncesi ve sonrası ovaryumda oluşan fonksiyonel ve histolojik değişiklikler değerlendirildi. Materyal ve Metod: Preoperatif (otografttan bir ay önce) ve postoperatif (otograftan iki ay sonra) östradiol (E2) ve progesteron (P) seviyeleri ölçüldü, akabinde histolojik değerlendirmeler ışık mikroskobuyla yapıldı. Bir ay sonra, tavşanlarda ovaryum otograftı yapıldı. Sonuçlar: Ovaryum dokularının %80"inde transplante ovaryum örnekleri gözlendi. Preoperatif ve postoperatif P değerleri bazaldan geç periyoda doğru artış gösterdi ve farklar istatistiksel olarak anlamlıdır. Bazaldan geç periyoda doğru preoperatif ve postoperatif E2 değerleri arasında istatistiksel olarak anlamlı bir fark yoktur. Her üç zaman periyodunda da preoperatif P değerleri postoperatif P değerlerinden istatiktiksel olarak anlamlı şekilde yüksektir. Preoperatif E2 değeri postoperatif E2 değerine göre yüksektir fakat bu fark istatistiksel olarak anlamlı değildir. Tartışma: Eğer otograft işlemleri mikrocerrahi yöntemleri kullanılarak gerçekleştirilirse, postoperatif periyottaki ovaryum otograftları preoperatif periyotta olduğu gibi hormonal fonksiyon ve morfoloji gösterirler.

Anahtar Kelimeler

Ovaryum otograftı, ovaryum hormonal fonksiyonu, ovaryum morfolojisi

Kaynakça

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