Pankreas duktal adenokarsinomunda NF-Kappa B ekspresyonu

Year 2019, Volume: 44 Issue: 2, 494 - 501, 30.06.2019

Deniz Tunçel , N. Ümit Bayol

https://doi.org/10.17826/cumj.481396

Abstract

Amaç: Bu çalışmada, NF-KB kompleksinin alt grubu olan p65 ve fosfo-IKB-alfa antikor ile NF-KB’nin aktivasyonu durumu araştırılmıştır.

Gereç ve Yöntem: Doku mikroarray yöntemi ile 107 olgu üzerinden yapılan çalışmada antikorun boyanması, boyanma şiddeti ve yüzdesi beraber değerlendirilerek skorlandı. Cell Signaling Technology ürünlerinden p65 ve fosfo-IKB-alfa(ser32/36) kullanıldı. Boyanma skorlarının arşiv bilgileri doğrultusunda cerrahi sınır, lenf bezi durumu, evre, tümör boyutu ve klinik bilgiler ışığında genel yaşam süresi ile korelasyonu araştırıldı. 

Bulgular: P65 nükleer, p-IkB-alfa sitoplazmik boyanma paterni izledi. Karsinomda, tümör içermeyen bölgelerle kıyaslandığında boyanma belirgin oranda şiddetli olup ve NF-KB aktivitesinde artış mevcuttu. NF-kB(p65) ekspresyonu, lenf bezi durumu ile anlamlı bir korelasyon göstermekle birlikte  yaş, ırk, cinsiyet, tümör lokalizasyonu, tümör boyutu, tümör derecesi ve genel yaşam süresi ile NF-KB(p65), pIkBalfa arasında korelasyon izlenmedi.

Sonuç: Lenf bezi durumu ile NF-KB aktivitesinin korelasyonu tümörün invaziv olma özelliğini ön plana çıkartmakta olup pankreas karsinogenezinde erken aşamalarda görülmesi tedavi açılımlarının geliştirilmesi açısından hedef molekül olabileceğini düşündürmektedir.

Keywords

NF-Kappa B, pankreas

NF-Kappa B expression in pancreatic ductal carcinoma

Abstract

Purpose: In this study we investigated the expression of the p65 subunit of the nuclear factor-kappaB (NF-kB)  complex and  the activation status of NF-kB by phospho-IkB-alpha antibody.

Materials and Methods: A tissue microarray based on material obtained from 107 patients was utilized. The antibody staining was scored by combining staining intensity with percentage of tumor staining. The antibodies used were NF-kB p65 and phospho-IkB-alpha(ser32/36), both from Cell Signaling Technology. The staining scores were correlated with the archival data available on some patients on margin and lymph node status, stage, tumor size, as well as clinical data including survival.

Results: The staining was nuclear (p65) and cytoplasmic (p-IkBalpha) respectively. In general there was an increased expression and activation of NF-kB in the carcinomas, compared to non-tumoral regions. None of the markers had a significant correlation with the overall survival. NF-kB(p65) expression had a correlation with positive lymph node status.

Conclusion: The correlation with the positive lymph node status suggests a role in invasive properties of the tumor. Activation of NF-kB is most likely an early event in pancreatic carcinogenesis. Despite the lack of an effect on overall survival, due to its increased activation in pancreatic cancer, NF-kB is still a good target for therapeutic interventions.

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