Effect of vitamin E on kidney histomorphology in rats exposed to bisphenol A

Yıl 2020, Cilt: 45 Sayı: 4, 1411 - 1417, 27.12.2020

Mustafa Yıldız Özay Güleş Murat Boyacıoğlu Şadiye Kum

Öz

Purpose: The aim of this study was to determine the effects of vitamin E on renal histomorphology in rats exposed to bisphenol A (BPA).
Materials and Methods: The rats were randomly divided into four groups as sham, vitamin E, BPA and vitamin E+BPA. The applications of vitamin E and BPA were continued for three weeks. Crossman's triple staining method was applied to the sections obtained from kidney tissues for histomorphological examinations at the end of the experiment. Also, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured in kidney tissue.
Results: The number of corpusculum renis, diameters of proximal tubule, distal tubule, ascending limb of Henle’s loop and collecting duct as well as SOD activity significantly decreased in BPA group compared to sham group. Also, the number of corpusculum renis, diameters of ascending limb of Henle’s loop and collecting duct as well as SOD activity significantly increased in vitamin E+BPA group compared to BPA group. On the other hand, MDA level significantly increased in BPA group compared to sham group and it significantly decreased in vitamin E+BPA group compared to BPA group.
Conclusion: BPA may cause histomorphological and oxidative changes in the kidney. In addition, vitamin E may contribute to the preservation of renal tissue by reducing oxidative damage in BPA exposure.

Anahtar Kelimeler

bisphenol A, rat, vitamin E

Bisfenol A'ya maruz kalan sıçanlarda E vitamininin böbrek histomorfolojisi üzerine etkisi

Öz

Amaç: Sunulan çalışmada, bisfenol A (BPA)'ya maruz bırakılan sıçanlarda E vitamininin böbrek histomofolojisi üzerine etkilerinin belirlenmesi amaçlandı.
Gereç ve Yöntem: Sıçanlar sham, E vitamini, BPA ve E vitamini+BPA olmak üzere rastgele dört gruba ayrıldı. E vitamini ve BPA uygulamaları üç hafta boyunca sürdürüldü. Deney sonunda böbrek dokularından elde edilen kesitlere histomorfolojik incelemeler için Crosman’ın üçlü boyama yöntemi uygulandı. Ayrıca böbrek dokusunda süperoksit dismutaz (SOD) aktivitesi ile malondialdehit (MDA) seviyesi ölçüldü.
Bulgular: Böbrekte korpuskulum renis sayısı, proksimal tübül, distal tübül, çıkan henle ve toplayıcı kanal çapları ile SOD aktivitesinin sham grubuna göre BPA grubunda anlamlı olarak azaldığı saptandı. Ayrıca korpuskulum renis sayısı, çıkan henle ve toplayıcı kanal çapları ile SOD aktivitesinin BPA grubuna göre E vitamini+BPA grubunda anlamlı olarak arttığı gözlendi. Diğer taraftan, MDA seviyesinin sham grubuna göre BPA grubunda anlamlı olarak arttığı, BPA grubuna göre E vitamini+BPA grubunda ise anlamlı olarak azaldığı tespit edildi.
Sonuç: BPA, böbrekte histomorfolojik ve oksidatif değişikliklere neden olabilir. Bununla birlikte, E vitamini BPA maruziyetinde oksidatif hasarı azaltarak böbrek dokusunun korunmasına katkı sağlayabilir.

Anahtar Kelimeler

Bisfenol A, E vitamini, histomorfoloji, böbrek

Kaynakça

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