Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis

Yıl 2022, Cilt: 47 Sayı: 2, 738 - 746, 30.06.2022

Gamze Taşkın Şenol Halil Mahir Kaplan Neslihan Boyan Özkan Oğuz Ergin Şingirik

https://doi.org/10.17826/cumj.1091707

Öz

Purpose: This study aimed to investigate the protective effect of geraniol and vitamin C on the experimental hepatocellular carcinogenesis (HCC) model by inducing FL83B hepatocyte cell lines with diethylnitrosamine (DENA).
Materials and Methods: The cells prepared in the medium were incubated with DENA (5 μM), geraniol (5 μM), and vitamin C (50 μM) for 48 hours in an incubator at 37 °C and 5% CO2. Groups were designed as follows: Group 1 (Control), group 2 (DENA Control), group 3 (DENA+Geraniol), group 4 (DENA+Vitamin C), and group 5 (DENA+Geraniol+Vitamin C) on standard cell culture plates. Six plates from each experimental group were studied. After the homogenization was centrifuged, analyses of pathway mediators NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX were performed by the Elisa method.
Results: The expression of Bax, caspase-3, COX-2, NFkB, GADD153, AIF, and GRP78 increased in cancer cells when compared to group 1 and decreased in other groups where antiproliferative agents were applied. Bcl-2 expression is decreased when compared to group 1, and expression is increased in other groups where antiproliferative agents are applied.
Conclusion: There was a significant hepatoprotective effect in the groups administered geraniol+vitamin C on pathway mediators in a DENA-induced HCC model.

Anahtar Kelimeler

Cancer, cell culture, hepatocytes, liver, mediators, pathway

Destekleyen Kurum

Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Bİrimi

Proje Numarası

TDK-2017-9717

Teşekkür

The authors thanks to Ahmet Hilmi Yücel, Prof. Dr., who suddenly passed away and had a huge contribution to the study that is in the working phase and thanks for the using laboratory facilities to the Cukurova University Department of Medical Pharmacology.

Geraniol ve vitamin C’nin dietilnitrozamin kaynaklı deneysel hepatoselüler karsinogenez üzerindeki etkisi

Öz

Amaç: Bu çalışmanın amacı dietilnitrozamin (DENA) ile FL83B hepatosit hücre hattını indükleyerek oluşturulan deneysel hepatosellüler karsinogenezis (HSK) modelinde geraniol ve vitamin C'nin koruyucu etkisini araştırmaktır.
Gereç ve Yöntem: Bu amaçla çalışmamızda besiyerinde hazırlanan hücreler DENA (5 μM), geraniol (5 μM) ve vitamin C (50 μM) ile 37 °C sıcaklıkta ve %5’lik CO2‘li etüvde 48 saat inkübe edildi. Hücreler inkübe edildikten sonra gruplar standart hücre kültürü plakalarında Grup 1 (Kontrol), grup 2 (DENA Kontrol), grup 3 (DENA+Geraniol), grup 4 (DENA+Vitamin C) ve grup 5 (DENA+Geraniol+Vitamin C) olmak üzere beş gruba ayrıldı. Her deney grubundan altı plaka çalışıldı. Homojenatlar santrifüj edildikten sonra yolak mediyatörlerinden NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX-2 analizleri Elisa metodu ile yapıldı.
Bulgular: Kanser hücrelerinde Bax, caspase-3, COX-2, NFkB, GADD153, AIF ve GRP78 ekspresyonu grup 1 ile kıyaslandığında artmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyonun azalmakta olduğu belirlenmiştir. Bcl-2 ekspresyonu grup 1 ile kıyaslandığında azalmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyon artmaktadır.
Sonuç: Bu çalışmada, DENA ile indüklenen HCC modelinde geraniol ve vitamin C uygulanan gruplarda yolak mediyatörleri aracılığıyla önemli bir hepatoprotektif etki olduğu gözlendi.

Anahtar Kelimeler

Kanser, hücre kültürü, hepatositler, karaciğer, mediyatör, yolak

Proje Numarası

TDK-2017-9717

Kaynakça

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