Association between surfactant protein B gene locus and acute bronchiolitis in infants

Year 2022, Volume: 47 Issue: 4, 1440 - 1446, 28.12.2022

Sümeyya Deniz Aybek , Ömer Ateş , Saime Sezer Sondaş , Ali Gül , Şahin Takçı , Buket Altıntaş Seyyah

https://doi.org/10.17826/cumj.1124468

Abstract

Purpose: The aim of this study was to investigate whether there is a relationship between surfactant protein B (SFTPB) C1580T polymorphism and acute bronchiolitis.
Materials and Methods: The study analyzed the allele frequency and genotype distribution for the SFTPB C1580T polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique in 103 acute bronchiolitis infants and 102 healthy infants.
Results: The results showed no association between SFTPB C1580T polymorphism and clinical characteristics of acute bronchiolitis. The distribution of the CT genotype was higher in acute bronchiolitis infants (43%) than in healthy subjects (39%) and distribution of the TT genotype was found lower in acute bronchiolitis infants (38%) than in healthy subjects (41%). No significant differences in genotype distribution and allele frequency for the SFTPB C1580T polymorphism were found between case group and control group
Conclusion: SFTPB C1580T gene polymorphism plays no important role in susceptibility to acute bronchiolitis. Further work on the relevance of SFTPB C1580T polymorphism in larger cohorts will require validating our results.

Keywords

Acute bronchiolitis, genetic polymorphism, SFTPB gene, surfactant protein

Supporting Institution

Gaziosmanpaşa University Scientific Research Projects

Project Number

2015/59

Bebeklerde akut bronşiolit ve surfaktan protein B gen lokusu arasındaki ilişki

Abstract

Amaç: Çalışmamızda surfaktan protein B (SFTPB) C1580T polimorfizmi ile akut bronşiolit arasında bir ilişki olup olmadığını araştırmayı amaçladık.
Gereç ve Yöntem: Bu çalışmada, polimeraz zincir reaksiyonu- restriksiyon parça uzunluk polimorfizmi (PZR-RFLP) tekniği kullanılarak 103 akut bronşiolitli bebekte ve 102 sağlıklı bebekte SFTB C1580T gen polimorfizminin alel sıklığı ve genotip dağılımı analiz edildi.
Bulgular: Bu sonuçlar SFTPB C1580T polimorfizmi ve akut bronşiolitin klinik özellikleri arasında bir ilişki olmadığını gösterdi. CT genotipinin dağılımı akut bronşiolitli bebeklerde (%43) kontol grubuna (%39) göre daha yüksektir ve TT genotipinin dağılımı akut bronşiolitli bebeklerde (%38) kontol grubuna (%41) göre daha düşüktür. Hasta ve kontrol grubu arasında SFTPB C1580T polimorfizmi için genotip dağılımı ve allel sıklığında önemli bir fark yoktur.
Sonuç: SFTPB C1580T gen polimorfizminin akut bronşiolite yatkınlıkta önemli bir rolü olmadığı saptanmıştır. Çalışma sonuçlarımızın doğrulanabilmesi için SFTPB C1580T polimorfizminin ilişkisinin ileri ve daha büyük kohort çalışmaları yapılarak değerlendirilmesine ihtiyaç vardır.

Keywords

Akut bronşiolit, genetik polimorfizm, SFTPB geni, surfaktan protein, Acute bronchiolitis, genetic polymorphism, SFTPB gene, surfactant protein

Project Number

2015/59

References

• 1. Ravaglia C, Poletti V. Recent advances in the management of acute bronchiolitis. F1000Prime Reports. 2014; 6-103.
• 2. Bush A, Thomson AH. Acute bronchiolitis. BMJ. 2007;335-1037.
• 3. Lo¨fgren J, Ra¨met M, Renko M, Marttila R, Hallman M. Association between Surfactant Protein A Gene Locus and Severe Respiratory Syncytial Virus Infection in Infants. The Journal of Infectious Diseases. 2002;185:283–9.
• 4. Fjærli H, Farstad T, Rød G, Ufert GK, Gulbrandsen P, Nakstad B. Acute bronchiolitis in infancy as risk factor for wheezing and reduced pulmonary function by seven years in Akershus County, Norway. BMC Pediatrics. 2005; 5-31 doi:10.1186/1471-2431-5-31.
• 5. Barreira ER, Precioso AR, Bousso A. Pulmonary Surfactant in Respiratory Syncytial Virus Bronchiolitis: The Role in Pathogenesis and Clinical Implications. Pediatric Pulmonology. 2011;46:415–420.
• 6. van de Wetering JK, van Golde LMG, Batenburg JJ. Collections:players of the innate immune system. Eur J Biochem. 2004;271:1229–1249.
• 7. Puthothu B, Forster J, Heinze J, Heinzmann A, Krueger M. Surfactant protein B polymorphisms are associated with severe respiratory syncytial virus infection, but not with asthma. BMC Pulmonary Medicine. 2007; 7:6 DOI:10.1186/1471-2466-7-6.
• 8. Jat KR, Chawla D. Surfactant therapy for bronchiolitis in critically ill infants. Cochrane Database Syst Rev. 2012;9:CD009194 DOI: 10.1002/14651858.
• 9. Dargaville PA, South M, McDougall PN. Surfactant abnormalities in infants with severe viral bronchiolitis. Archives of Disease in Childhood. 1996;75:133-136.
• 10. Petrarca L, Jacinto T, Nenna R. The treatment of acute bronchiolitis: past,present and future. Breathe. 2017; 13: e24–e26.
• 11. Christmann U, Buechner-Maxwell VA, Witonsky SG, Hite RD. Role of Lung Surfactant in Respiratory Disease: Current Knowledge in Large Animal Medicine. J Vet Intern Med. 2009;23:227–242.
• 12. Floros J, Fan R, Diangelo S, Guo X, Wert J, Luo J. Surfactant protein (SP) B associations and interactions with SPA in white and black subjects with respiratory distress syndrome. Pediatr Int. 2001; 43(6): 567–576.
• 13. Puthothu B, Krueger M, Heinze J, Forster J, Heinzmann A. Haplotpyes of Surfactant Protein C are associated with common paediatric lung diseases. Pediatr Allergy Immunol. 2006; 17:572-577.
• 14. Gul A, Takçı Ş, Seyyah BA, Yılmaz R. Independent Predictors of Severity and Hospitalization in Acute Bronchiolitis: Neutrophil/Lymphocyte Ratio and Mean Platelet Volume. J Pediatr Infect Dis (EFirst). 2018; doi: 10.1055/s-0038-1657784.
• 15. Lin Z, Pearson C, Chinchilli V, Pietschmann SM, Luo J, Pison U, Floros J. Polymorphisms of human SP-A, SP-B, and SP-D genes: association of SP-B Thr131Ile with ARDS. Clin Genet. 2000; 58: 181–191.
• 16. Wang SZ, Doyle IR, Nicholas TE, Forsyth KD. Plasma surfactant protein-B is elevated in infants with respiratory syncytial virus-induced bronchiolitis. Pediatr Res. 1999;46(6):731-734. doi:10.1203/00006450-199912000-00023.
• 17. Bria M, Coates LEC. Wheezing in Infants: Bronchiolitis. In: Kliegman RM, ed. Nelson Textbook of Pediatrics. Twentieth ed. Canada: Elsevier. 2016;2044-50.
• 18. Wang EE, Law BJ, Boucher FD, et al. Pediatric Investigators Collaborative Network on Infections in Canada (PICNIC) study of admission and management variation in patients hospitalized with respiratory syncytial viral lower respiratory tract infection. J Pediatr. 1996;129(3):390-5.
• 19. Ruicheng HU, Yongjian XU, ZHANG Z. Surfactant Protein B 1580 Polymorphism Is Associated with Susceptibility to Chronic Obstructive Pulmonary Disease in Chinese Han Population. Journal of Huazhong University of Science and Technology. 2004;24 (3):216-218.
• 20. Pientong C, Ekalaksananan T, Teeratakulpisarn J, Tanuwattanachai S, Kongyingyoes B, Limwattananon C. Atypical bacterial pathogen infection in children with acute bronchiolitis in north east Thailand. Journal of Microbiology, Immunology and Infection. 2011; 44: 95-100.
• 21. Øymar K, Skjerven HO, Mikalsen IB. Acute bronchiolitis in infants, a review. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine. 2014, 22:23.
• 22. Sigurs N, Aljassim F, Kjellman B, et al. Asthma and allergy patterns over 18 years after severe RSV bronchiolitis in the first year of life. Thorax. 2010;65:1045e1052.
• 23. Murray J, Bottle A, Sharland M, et al. Risk factors for hospital admission with RSV bronchiolitis in England: a population-based birth cohort study. PLoS One. 2014; 26;9(2):e89186.
• 24. Siezen CL, Bont L, Hodemaekers HM, et al. Genetic susceptibility to respiratory syncytial virus bronchiolitis in preterm children is associated with airway remodeling genes and innate immune genes. Pediatr Infect Dis J. 2009; 28(4):333-5.
• 25. Haczku A. Protective role of the lung collectins surfactant protein A and surfactant protein D in airway inflammation. J Allergy Clin Immunol. 2008;122:861–879.
• 26. Lahti M, Lofgren J, Marttila R, et al.Surfactant protein D gene polymorphism associated with severe respiratory syncytial virus infection. Pediatr Res. 2002;51:696–699.
• 27. Hoebee B, Bont L, Rietveld E, et al. Influence of Promoter Variants of Interleukin-10, Interleukin-9, and Tumor Necrosis Factor–a Genes on Respiratory Syncytial Virus Bronchiolitis. The Journal of Infectious Diseases. 2004; 189:239–47.
• 28. Ge L, Liu X, Chen R, Xu Y, Zuo YY, Cooney RN, Wang G. Differential susceptibility of transgenic mice expressing human surfactant protein B genetic variants to Pseudomonas aeruginosa induced pneumonia. Biochemical and Biophysical Research Communications. 2016;469: 171-175.
• 29. Schoenborn, MC.Human Surfactant Protein B Expression in Humanized Transgenic Mice. Syracuse University Honors Program Capstone Projects. 2014; Paper 767.
• 30. Ezzeldin N, Shalaby A, Saad-Hussein A, Ezzeldin H, Lebedy DE, Farouk H, Kandil DM. Association of TNF- α –308G/A, SP-B 1580 C/T,IL-13 –1055 C/T gene polymorphisms and latent adenoviral infection with chronic obstructive pulmonary disease in an Egyptian population. Arch Med Sci. 2012; 8 (2): 286-295.
• 31. To KKW, Zhou J, Song YQ, et al. Surfactant Protein B Gene Polymorphism Is Associated With Severe Influenza. CHEST. 2014; 145(6):1237–1243.