Isolation of Cucurbit Bacterial Disease Agents Affecting Commercial Nurseries in Adana and Biological Control of Cucumber Angular Leaf Spot Disease

Yıl 2024, Cilt: 39 Sayı: 2, 319 - 335, 30.12.2024

Merve Okur , Yesim Aysan

Öz

In this study, the identification of cucurbit bacterial pathogens and the biological control of cucumber angular leaf spot disease using antagonistic bacterial strains were investigated in commercial nurseries in Adana. Among the strains obtained from disease surveys in Adana, 27 were identified as Pseudomonas syringae pv. lachrymans, and 14 were identified as Pseudomonas syringae pv. syringae. In the biological control studies, the in vitro antibacterial effects, phosphorus solubilization, siderophore production, and indole-3-acetic acid (IAA) production capabilities of 298 candidate antagonistic bacteria against Pseudomonas syringae pv. lachrymans were examined, leading to the selection of 15 antagonistic bacteria for in vivo seed applications. In the first seed trial, 12 effective antagonistic bacteria achieved a 21-78% reduction in disease incidence. In the second seed trial, significant disease suppression was confirmed when five antagonistic bacteria (AD 4-2, OG1-2, OG7-7, NÇ-KK, X-77) and two combinations (OG1-2 and OG7-7) were applied. The antagonistic bacterial strains that demonstrated effective disease suppression were identified as Providencia rettgeri, Bacillus amyloliquefaciens, Bacillus mojavensis, Bacillus pumilus, and Enterococcus casseliflavus.

Anahtar Kelimeler

Pseudomonas syringae pv. lachrymans, antagonist, IAA, Siderophore, Antimicrobial effect

Proje Numarası

FYL-2023-15812

Ticari Fideliklerde Sorun Olan Kabakgil Bakteriyel Hastalık Etmenlerinin İzolasyonu ve Hıyar Köşeli Yaprak Leke Hastalığının Biyolojik Mücadelesi

Öz

Pseudomonas syringae pv. lachrymans’ın neden olduğu Hıyar Köşeli Yaprak Leke Hastalığı kabakgillerde verim kayıplarına neden olan önemli bir bakteriyel hastalıktır. Bu çalışmada, Adana’da bulunan ticari fideliklerde sorun olan kabakgil bakteriyel hastalık etmenlerinin tanılanması ve yerel antagonistleri kullanarak Hıyar Köşeli Yaprak Leke Hastalığı’nın biyolojik mücadelesi araştırılmıştır. Adana’da yapılan ziyaretlerde 27 izolat Pseudomonas syringae pv. lachrymans ve 14 izolat Pseudomonas syringae pv. syringae olarak tanılanmıştır. Biyolojik mücadele çalışmalarında 298 adet aday antagonist bakterinin in vitro antimikrobiyal etkisi, fosforu çözme, siderofor üretimi ve IAA üretme yeteneği incelenmiş ve biyolojik tohum uygulamaları için 15 adet antagonist bakteri seçilmiştir. kurulan birinci biyolojik tohum denemesinde, 12 etkili antagonist hastalığı %21-78 oranında baskılamıştır. İkinci tohum denemesinde, 5 adet (AD 4-2, OG1-2, OG7-7, NÇ-KK, X-77) antagonist ve bir çift kombinasyon (OG1-2 ve OG7-7) kullanıldığında hastalığın etkili bir şekilde baskılandığı tekrar teyit edilmiştir. Başarılı antagonistler Providencia rettgeri, Bacillus amyloliqofasciens, Bacillus mojavensis, Bacillus pumilus ve Enterecoccus casseliflavus olarak tanılanmıştır.

Anahtar Kelimeler

Pseudomonas syringae pv. lachrymans, Yerel antagonist, IAA, Siderofor, Antimikrobiyal etki

Destekleyen Kurum

Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FYL-2023-15812

Teşekkür

Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimine FYL-2023-15812 nolu projeye verdiği maddi desteğinden dolayı teşekkür ederiz.

Kaynakça

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