ITO Yüzeylerinde Elektrodepozitlenen ZnO Nanoyapılar: Kolesterol Biyosensör Uygulamaları için Etkinliklerinin Araştırılması

Öz

Bu çalışmada, ZnO nanoyapıları, kolesterol tespiti için ITO/cam altlıklar üzerine elektrodepozitlenen Zn'nin elektrokimyasal anodizasyonu ile hazırlanmıştır. Geliştirilen ZnO nanoyapıların Kolesterol oksidaz (ChOx) enziminin tespitindeki etkinliği döngüsel voltametri yöntemi ile belirlenmiştir. XRD ve SEM sonuçları, çeşitli parametrelerle anodizasyon yöntemiyle hazırlanan ZnO nanoyapıların sentezini doğrulamıştır. Elektrodepozisyon ve anodizasyon süresinin morfoloji üzerindeki etkisi gözlemlenmiştir. ZnO/Zn/ITO/cam ve Pt/ZnO/Zn/ITO/cam elektrotların çeşitli kolesterol konsantrasyonlarına sahip elektrolitlerdeki döngüsel voltametrisi gerçekleştirilmiştir. Elde edilen Pt/ZnO/Zn/ITO/cam yapılı elektrotun tespit limiti 0,965x10-3M olarak hesaplanmıştır. Elde edilen katmanlı yapıya sahip malzeme, biyomedikal uygulamalarda elektrokimyasal sensörlerde ve biyosensörlerde potansiyel uygulamalara sahip olabilir. Bu çalışma, biyosensör performansına ek olarak, ZnO tabanlı biyosensörlerin geliştirilmesi için pahalı altyapı ve hammadde maliyeti gerektirmeyen yeni bir yaklaşım önermekte ve gelecek çalışmalarda yapılacak iyileştirmelerle daha düşük tespit limitlerine sahip yüksek hassasiyetli biyosensör elektrotlarının geliştirilmesini mümkün kılmaktadır.

Anahtar Kelimeler

• ZnO nanoyapılar
• elektrokimyasal anotlama
• kolesterol
• biyosensör

Proje Numarası

2019.KB.FEN.029

Electrodeposited ZnO Nanostructures on ITO Surfaces: Exploring Their Efficacy for Cholesterol Biosensing Applications

Öz

In this study, ZnO nanostructures were prepared by electrochemical anodization of electrodeposited Zn on ITO/glass substrates for cholesterol detection. The efficiency of the developed ZnO nanostructures in the detection of the Cholesterol oxidase (ChOx) enzyme was determined by the cyclic voltammetry method. The XRD and SEM results confirmed the synthesis of ZnO nanostructures prepared by the anodization method with various parameters. The effect of electrodeposition and anodization time on the morphology was observed. Cyclic voltammetry of ZnO/Zn/ITO/glass and Pt/ZnO/Zn/ITO/glass electrodes in electrolytes with various cholesterol concentrations was performed. The detection limit of the obtained Pt/ZnO/Zn/ITO/glass structured electrode was calculated as 0.965x10-3M. The resulting material with a layered structure may have potential applications in electrochemical sensors and biosensors in biomedical applications. In addition to biosensing performance, this study proposes a new approach for the development of ZnO-based biosensors that does not require expensive infrastructure and raw material costs, making it possible to develop high-sensitivity biosensor electrodes with lower detection limits with improvements to be made in future studies.

Anahtar Kelimeler

• Keywords: ZnO nanostructures
• electrochemical anodization
• cholesterol
• biosensor

Destekleyen Kurum

Dokuz Eylül University Scientific Research Coordination Unit. Project Number: 2019.KB.FEN.029

Proje Numarası

2019.KB.FEN.029

Etik Beyan

"This article does not require ethics committee approval." "This article has no conflicts of interest with any individual or institution."

Teşekkür

The authors are indebted to the infrastructural support from Dokuz Eylül University and İzmir Katip Çelebi University. This study was supported by the Dokuz Eylül University Scientific Research Coordination Unit. Project Number: 2019.KB.FEN.029.

Kaynakça

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