COMPARISON OF TWO CHROMOGENIC MEDIA FOR YEAST IDENTIFICATION

Yıl 2018, , 23 - 31, 18.05.2018

M. Cem Ergon Buğse Tunç Mine Doluca Dereli

https://doi.org/10.5505/deutfd.2018.26121

Öz

Objective: Usage of catheter, prosthesis, treatment with extended
spectrum antibiotics for a long time, organ transplantation and
immunodeficiency enhanced invasive Candida infections. Quite a few chromogenic media
were developed for rapid identification of yeasts, recently. We aimed to
compare performances of CHROMagar Candida (CHROMagar, France) and Rosachrom
Candida Agar II (Gül Biyoloji Laboratuvarı, Turkey) for the identification of
the yeast species.

Material and Method: Totally 66 isolates including 22 C. glabrata, 16 C. tropicalis,
10 C. albicans, nine C. parapsilosis, four C. kefyr, two Saprochaete capitata, two Trichosporon
spp., one C. lusitaniae isolated
from various clinical specimens between September–October 2015 in Mycology
Laboratory of our hospital were included. The colonies of the isolates were
evaluated by considering the colony morphology, color and the existence of halo
around the colony by two different people after 24 and 48 hours.

Results: The isolates grew well on both chromogenic media, and
showed clearly distinguished colonies. The sensitivity and specificity values
for C. albicans were detected as 100%
for CHROMagar Candida; 70-80% and 94.6-96.4% for Rosachrom Candida, at 24 and
48 hours, respectively. These values for C.
tropicalis were 100% for CHROMagar Candida; 43.7–50 % and 98-100 % for
Rosachrom Candida, respectively.

Conclusion: CHROMagar Candida which
can identify, C. albicans, C. tropicalis and C. glabrata with high sensitivity and specificity rates was
concluded as a reliable medium for identification of Candida species. Rosachrom
Candida may be thought to be an alternative to CHROMagar Candida for C. albicans identification but its
performance in identification of C.
tropicalis was not found sufficient.

Anahtar Kelimeler

Identification of Yeasts, Chromogenic Media, Chromagar Candida, Rosachrom Candida

Maya mantarlarının identifikasyonunda iki farklı kromojenik besiyerinin karşılaştırılması

Öz

Amaç: Kateter, protez kullanımı,
uzun süren geniş spektrumlu antibiyotik tedavileri, organ nakli ve immün
yetersizlik gibi faktörler invazif Candida enfeksiyonlarında artışa neden
olmaktadır. Son yıllarda hızlı maya identifikasyonunu sağlamak amacıyla birçok kromojenik
besiyeri geliştirilmiştir. Bu çalışmada maya suşlarının tür düzeyinde
tanımlanmasında iki farklı kromojenik besiyeri olan CHROMagar Candida
(CHROMagar, Fransa) ile Rosachrom Candida Agar II’nin (Gül Biyoloji
Laboratuvarı, Türkiye) performanslarının karşılaştırılması amaçlandı.

Gereç ve Yöntem: Çalışmaya, Eylül – Ekim 2015
tarihleri arasında hastanemiz Mikoloji Laboratuvarında çeşitli klinik
örneklerden soyutlanan 22 C. glabrata,
16 C. tropicalis, 10 C. albicans, dokuz C. parapsilosis, dört C. kefyr, iki Saprochaete capitata, iki Trichosporon
spp., bir C. lusitaniae olmak
üzere toplam 66 suş alındı. Suşların her iki kromojenik besiyerindeki koloni
morfolojisi ile rengi ve koloni etrafında halenin varlığı, 24. ve 48. saatlerde
iki farklı kişi tarafından değerlendirildi. 

Bulgular: Çalışmaya alınan izolatların
tümü her iki kromojenik besiyerinde de ayırt edilebilen koloniler şeklinde iyi
üreme gösterdi. C. albicans için
24.-48. saatlerde duyarlılık ve özgüllük değerleri sırası ile CHROMagar Candida
için %100 ve Rosachrom Candida için %70-80 ile %94,6-96,4 olarak saptandı. Bu
değerler, C. tropicalis izolatlarında
sırası ile CHROMagar Candida için %100; Rosachrom Candida için %43,7–50 ve
%98-100 idi. 

Sonuç: CHROMagar Candida, C. albicans, C. tropicalis ve C. glabrata
türlerini doğru identifiye etmede gösterdiği yüksek duyarlılık ve özgüllük
oranları nedeniyle Candida türlerinin identifikasyonu için uygun bir besiyeri
olarak belirlendi. Rosachrom Candida besiyerinin ise, C. albicans identifikasyonunda CHROMagar Candida besiyerine
alternatif olarak düşünülebileceği ancak C.
tropicalis identifikasyonundaki performansının yeterli olmadığı görüşüne
varıldı. 

Anahtar Kelimeler

Maya Mantarı İdentifikasyonu, Kromojenik Besiyeri, Chromagar Candida, Rosachrom Candida

Kaynakça

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