Determination of Antioxidant Effect of Walnut (Juglans regia L.) on Lung and Muscle Tissue
Öz
This study was to investigate the antioxidant role of Juglans regia L. (walnut) against ethanol-induced oxidative stress in lung and muscle tissues. Female Albino 36 rats were divided into six groups and treated as follows: I (control), II (20% ethanol), III (10%walnut), IV (20% ethanol + 10%walnut), V (5% walnut) and VI (20% ethanol + 5% walnut). The rats were sacrificed after 50 days of administration and the tissues were analyzed after isolation. According to the results, it was found that the increased malondialdehyde (MDA) content owing to the alcohol-induced oxidative stress in both tissues decreased in the walnut treated tissues. In addition alcohol and alcohol + walnut treatment of nutritional supplemented rats changed catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and superoxide dismutase (SOD) content and antioxidant defence system (ADS) components compared to control rats. As a result, ethanol has been shown to cause fluctuations in ADS components as a result of oxidative stress in rats and it can be said that walnut has a curative effect on the this complications caused by oxidative stress.
Anahtar Kelimeler
Kaynakça
- Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S., Rezaiee, A., 2004. Pesticides and oxidative stress: a review. Medical Science Monitor, 10(6), 41-47.
- Aebi, H., 1974, Catalase, ‘‘In Methods of Enzymatic Analysis’’. In H. U. Bergemeyer (Ed.), Academic Press: New York 673-684.
- Aykac, G., Uysal, M., Yalcin, A. S., Koçak-Toker, N., Sivas, A., Öz, H., 1985. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology, 36, 71–76.
- Bagnyukova, T.V., Vasylkiv, O.Y., Storey KB, Lushchak VI., 2005. Catalase inhibition by amino triazole induces oxidative stress in goldfish brain. Brain Research, 1052(2), 180-6.
- Bati, B., Celik, I., Dogan, A., 2015. Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell Biochemistry and Biophysics, 71(2), 1191-1198.
- Beutler, E., Dubon, O.B., Kelly, M., 1963. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882–888.
- Bhor, V.M., Raghuram, N., Sivakami, S., 2004. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. The International Journal of Biochemistry & Cell Biology, 36(1), 89-97.
- Carlberg, I., Mannervik, B., 1975. Purification and characterization of the flavoenzyme glutathione reductase from rat live. Journal of Biological Chemistry, 250, 5475–5480.
- Celik, I., Temur, A., Isık, I., 2015. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum L.). Cell Biochem Biophys, 71, 1191–1198.
- Christaki. E., Bonos, E., Giannenas, I., Florou-Paneri, P., 2012. Aromatic plants as a source of bioactive compounds. Agriculture, 2, 228-243.