Antioxidative Effects of Boron Minerals in LPS-Induced Psoriasis Model

Öz

Objectives: Psoriasis is a chronic inflammatory skin disorder that substantially affects patients’ quality of life. Oxidative stress arises when there is a disruption in the balance between reactive oxygen species (ROS) and the body's antioxidant defense mechanisms, significantly contributing to its pathophysiology. This study aimed to investigate the effects of various boron compounds—borax, colemanite, boric acid, and ulexite—on oxidative stress markers in an LPS-induced psoriasis-like cell culture model using HaCaT keratinocytes. Methods: HaCaT cells were stimulated with LPS to induce a psoriasis-mimicking inflammatory response. Subsequently, cells were treated with different boron compounds. To evaluate antioxidant activity and lipid peroxidation, oxidative stress markers such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) were examined. Results: Boron compounds modulated oxidative stress parameters by enhancing SOD and GPx activities and reducing lipid peroxidation, as reflected by decreased MDA levels. Among the tested agents, colemanite and ulexite exhibited the strongest antioxidant activities, likely attributable to their distinct mineral compositions. Conclusion: Findings suggest that boron compounds hold therapeutic potential for reducing oxidative stress in psoriasis-like conditions. Further research is needed to clarify the underlying molecular mechanisms and to refine boron-based therapeutic applications.

Anahtar Kelimeler

• Psoriasis
• LPS
• boron compounds
• borax
• ulexit
• colemanite

LPS ile İndüklenen Psöriasis Modelinde Bor Minerallerinin Antioksidan Etkileri

Öz

Amaç: Psoriasis, hastaların yaşam kalitesini önemli ölçüde etkileyen kronik inflamatuvar bir deri hastalığıdır ve patogenezinde reaktif oksijen türleri (ROS) ile antioksidan savunma mekanizmaları arasındaki dengenin bozulduğu oksidatif stres kritik bir rol oynar. Bu çalışma, LPS ile psöriasis benzeri inflamatuar yanıt oluşturulan HaCaT keratinosit kültüründe bor bileşiklerinin (boraks, kolemanit, borik asit ve üleksit) oksidatif stres belirteçleri üzerindeki etkilerini araştırmayı amaçlamaktadır. Yöntemler: HaCaT hücrelerinde LPS ile inflamasyon indüklenmiş ve ardından farklı bor bileşikleri uygulanmıştır. Süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve malondialdehit (MDA) düzeyleri ölçülerek antioksidan enzim aktiviteleri ve lipit peroksidasyonundaki değişimler değerlendirilmiştir. Bulgular: Boron bileşiklerinin SOD ve GPx aktivitelerini artırarak antioksidan yanıtı güçlendirdiği, aynı zamanda MDA düzeylerindeki azalma ile lipit peroksidasyonunu azalttığı belirlenmiştir. Bileşikler arasında özellikle kolemanit ve üleksitin mineral yapılarından kaynaklanabilecek belirgin antioksidan etki gösterdiği saptanmıştır. Sonuç: Bu bulgular, bor bileşiklerinin psöriasis gibi oksidatif stresle ilişkili deri hastalıklarında terapötik potansiyel taşıdığını göstermektedir. Altta yatan moleküler mekanizmaların aydınlatılması ve bor temelli tedavi stratejilerinin optimize edilmesi için ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Psöriasis
• LPS
• boraks
• üleksit
• kolemanit.

Kaynakça

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