Apoptotic Effects of Reduced Brain Derived Neurotrophic Factor (BDNF) on Mouse Liver and Kidney
Öz
Objective: Brain-derived neurotrophic factor (BDNF) promotes the development and differentiation of neurons and synapses, as well as neuronal survival, by acting on specific neuronal groups in the central and peripheral nervous systems. However, the direct effect of BDNF on apoptosis in peripheral tissues is not known. The aim of this study was to investigate the relationship between BDNF and apoptosis, and the density and distribution of BDNF receptors in liver and kidney tissues by histological and immunehistochemical methods.
Methods: Seven wild-type and 7 BDNF heterozygous (reduced BDNF levels) male mice were used in the study. Caspase-3 and TUNEL immunehistochemical stainings were performed in order to investigate the presence of apoptosis in the liver and kidney tissues of the studied groups. Apoptosis-entering cells were counted and the groups were compared. Concentration and distribution of BDNF receptors, tropomyosin-related kinase B (TrkB) and nerve growth factor receptor p75 (NGFR p75), in liver and kidney tissues were also examined by immunehistochemical analyzes.
Results: As a result of Caspase-3 and TUNEL immunehistochemical staining, more cells were counted to enter the apoptotic process in sections of BDNF heterozygous group compared to control group (p<0.0001). In both groups TrkB and NGFR p75 receptors in liver and kidney tissues were determined in trace amounts, but there was no difference in intensity and distribution between the studied groups.
Conclusion: According to our histological and immunehistochemical stainings and statistical analysis of cell count between groups, it was found that BDNF is protective against apoptosis in liver and kidney. The lack of difference between the studied groups in terms of intensity and distribution of BDNF receptors, suggests that BDNF receptor distribution in the liver and kidney tissues may be different from the nervous system or that BDNF may differ in affinity for these receptors.
Anahtar Kelimeler
Kaynakça
- 1. Binder DK, Scharfman HE. Brain-derived neurotrophic factor. Growth Factors. 2004; 22:123-31.
- 2. Lewin GR, Barde YA. Physiology of neurotrophins. Annu Rev Neurosci. 1996; 19:289-317.
- 3. Barde YA, Edgar D, Thoenen H. Purification of a new neurotrophic factor from mammalian brain. EMBO Journal. 1982; 1:549-53.
- 4. Götz R, Köster R, Winkler C, et al. Neurotrophin-6 is a new member of the nerve growth factor family. Nature. 1994; 372:266-9.
- 5. Hallböök F, Ibanez CF, Persson H. Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary. Neuron. 1991; 6:845-58.
- 6. Hohn A, Leibrock J, Bailey K, et al. Identification and characterization of a novel member of the nerve growth factor/brain-derived neurotrophic factor family. Nature. 1990; 344:339-41.
- 7. Abidin I, Eysel UT, Lessmann V, et al. Impaired GABAergic inhibition in the visual cortex of brain-derived neurotrophic factor heterozygous knockout mice. J Physiol. 2008; 586:1885-901.
- 8. Acheson A, Conover JC, Fandl JP, et al. A BDNF autocrine loop in adult sensory neurons prevents cell death. Nature. 1995; 374:450-3.
Ayrıntılar
Birincil Dil
İngilizce
Konular
-
Bölüm
Araştırma Makalesi
Yazarlar
Gülay Hacıoğlu
Bu kişi benim
0000-0002-8528-2371
Selcen Aydın Abidin
Bu kişi benim
0000-0002-5843-5539
İsmail Abidin
Bu kişi benim
0000-0003-2510-9718
Yayımlanma Tarihi
5 Aralık 2017
Gönderilme Tarihi
5 Aralık 2017
Kabul Tarihi
-
Yayımlandığı Sayı
Yıl 2017 Cilt: 44 Sayı: 4