Sülforafanın ratlarda asetaminofenin-indüklediği nefrotoksisitede sistatin-c düzeylerine etkisi

Yıl 2016, Cilt: 43 Sayı: 3, 383 - 389, 01.09.2016

Eda Dokumacıoğlu Hatice Iskender Mustafa Sinan Aktas Basak Hanedan Ali Dokumacıoglu Tugba Mazlum Sen Sinan Saral

Öz

Amaç: Canlıların kimyasallara ve ilaçlara maruziyeti sıklıkla
karaciğer ve böbrek toksisitesi ile sonuçlanır. Toplum
ve hastane kaynaklı böbrek hastalıklarının önemli ve
büyük bir kısmını ilaçlar oluşturmaktadır. Çalışmamızda,
asetaminofen (APAP) ile ratlarda indüklenen nefrotoksisitede
sülforafanın (SFN) sistatin-C düzeyleri ve lipid peroksidasyonu
üzerine olan etkilerinin araştırılması amaçlanmıştır.

Yöntemler: 36 Sprague-Dawley sıçan her bir grup eşit
hayvan içerecek şekilde dört gruba ayrıldı. Gruplarımız,
kontrol grubu, SFN grubu, APAP grubu ve APAP+SFN
grubundan oluşmaktadır. SFN ve APAP+SFN grubundaki
sıçanlara tedavi için üç gün süresince 500 μg/kg dozunda
SFN oral gavaj yoluyla verildi. APAP ve APAP+SFN grubundaki
sıçanlara hepatotoksisite oluşturmak için APAP
sıcak salin içerisinde çözüldü ve çalışmanın son gününde
1g/kg olacak şekilde tek doz oral gavaj yoluyla uygulandı.
APAP+SFN grubunda, APAP uygulaması SFN uygulamasından
üç saat sonra yapıldı. APAP uygulamasından 24
saat sonra sıçanlar sakrifiye edildi.
Bulgular: APAP uygulamasının serum üre, kreatinin,
LDH ve BUN düzeylerini kontrol grubuna göre anlamlı
derecede arttığı gözlemlenmiştir (p<0.001). SFN uygulamasının
sistatin C düzeylerini ve lipid peroksidasyonunu
APAP grubuna göre azalttığı gözlemlenmiştir (p<0.05).
Sonuç: Sülforafanın, asetaminofen kaynaklı nefrotoksisitede
tedavi protokolüne eklenmesinin yarar sağlayacağını
düşünmekteyiz. Böbrek hastalıklarında önemli bir rol
oynayan sistatin C’nin bir biyomarker olarak rutine girebilmesi
için çeşitli çalışmalara ihtiyaç vardır

Anahtar Kelimeler

Asetaminofen, Sistatin C, Lipid peroksidasyonu, Nefrotoksisite

The effect of sulforaphane on the levels of serum cystatin-c in acetaminophen-induced nephrotoxicity in rats

Öz

Objective: The exposure of living creatures to drugs and chemicals often results in toxicity of liver and kidney. Drugs constitute an important and big part of the commu­nity and hospital-acquired kidney diseases. In this study, we investigated the effect of sulforaphane (SFN) on the levels of cystatin-C and lipid peroxidation on acetamino­phen (APAP)- induced nephrotoxicity in rats. Methods: Thirty-six Sprague-Dawley rats were separat­ed equally into four experimental groups: control group, SFN group, APAP group, and APAP + SFN group. In the experimental treatment groups APAP was administered oral gavage at 1 g/kg 3 h after SFN treatment in last day and, in the APAP + SFN group, SFN was administered oral gavage at a dose of 500 μg/kg exactly for three days. Rats were euthanized and sacrificed 24 h after APAP ad­ministration. Results: APAP administration showed to significant in­crease in serum BUN, creatinine, urea and LDH concen­trations as compared to the control datas indicating the induction of severe nephrotoxicity (p<0.001). SFN treat­ment significantly decreased the cystatin-C levels and lipid peroxidation compared to APAP group (p<0.05). Conclusion: The present study demonstrate that the at­tachment of SFN to the nephrotoxicity treatment protocol will be beneficial and further studies should be conducted for cystatin C which plays an important role in kidney tox­icity and disease to be routinized as a biomarker.

Anahtar Kelimeler

Acetaminophen, Cystatin-C, Lipid peroxidation, Nephrotoxicity

Kaynakça

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