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The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density

Yıl 2008, Cilt: 35 Sayı: 4, 240 - 244, 01.12.2008

Öz

The aim of this study was to investigate the effect of extremely low frequency magnetic field (ELF MF) on bone that exposed us in our daily life. In our study, sixty four male Wistar-Albino rats were used. The rats were exposed to ELF MF (50Hz, 1.5mT) during 4 hours/day for 45 days. The experimental rats were divided into eight groups (n=8 per group). The groups were as follows; only ELF MF was exposed to 1.group, the ELF MF was exposed to the 2., 3. and 4.groups with the doses manganese (Mn) of 3.75 mg/kg, 15mg/kg and 60mg/kg respectively, the 5., 6. and 7.groups received Mn with the doses of 3.75 mg/kg, 15mg/kg and 60mg/kg respectively and lastly the 8.group was used as a control group (cage control). Total bone mineral content (TBMC) and total bone mineral density (TBMD) of rats were determined by dualenergy X-ray absorptiometry (DXA). Measurements were recorded for all groups and the levels were compared with the control group. The levels of TBMC increased significantly in 3., 4. and 7. groups compare to control group (p

Kaynakça

  • Fadel MA. Growth assessment of children exposed to low frequency electromagnetic fields at the Abu Sultan area in Ismailia (Egypt). Anthropol Anz 2006; 64: 211-226.
  • Demsia G. Effect of 910-MHz electromagnetic field on rat bone marrow. Sci World J 2004; 4 Suppl 2: 48-54.
  • Rollwitz J. Fifty-hertz magnetic fields induce free radical formation in mouse bone marrow-derived promonocytes and macrophages. Biochim Biophys Acta 2004;1674: 231-238.
  • Sievert U. Effects of electromagnetic fields emitted by cellular phone on auditory and vestibular labyrinth. Laryngorhinootologie 2007; 86: 264-270.
  • Funk RH. Effects of electromagnetic fields on cells: physiological and therapeutical approaches and molecular mechanisms of interaction. A review. Cells Tissues Organs 2006; 182: 59-78.
  • Lirani AP. Evidences of physical agents action on bone metabolism and their potential clinical use. Arq Bras Endocrinol Metabol 2005; 49: 891-896.
  • Seaman RL. Comments on "Evaluation of interactions of electric fields due to electrostatic discharge with human tissue". IEEE Trans Biomed Eng 2005; 53: 1220.
  • Binderman I, Somjen D, Shimshoni Z, et al. Stimulation of skeletal-derived cell cultures by different electric field intensities is cell-specific. Biochim Biophys Acta 1985; 844:273-279.
  • Laughlan KA, Steiner UE The spin correlated radical pair as a reaction intermediate. Mol Phys 1991; 73:241
  • Aday WR, Lawrence AF. Nonlinear Electrodynamics in Biological Systems. Plenum, New York 1984, 3–22
  • Giugni TD, Braslan DL, Haigler HT. Electric field induced redistribution and postfield relaxation of epidermal growth factor receptors on A 431 cells. J Cell Biol 1987; 104:1291–1297
  • Chang K, Chang WH, Yu YH, Shih C. Pulsed electromagnetic field stimulation of bone marrow cells derived from ovariectomized rats affects osteoclast formation and local factor production. Bioelectromagnetics 2004; 25: 1 3441.
  • Rubin J, McLeod KJ, Titus L, et al. Formation of osteoclast-like cells is suppressed by low frequency, low intensity electric fields. J Orthopaed Res 1996;14:7-15.
  • Shankar VS, Simon BJ, Bax CM, et al. Effects of electromagnetic stimulation on the functional responsiveness of isolated rat osteoclasts. J Cell Physiol 1998;176:53744.
  • Chang K, Chang WH, Tsai MT, Shih C. Pulsed Electromagnetic Fields Accelerate Apoptotic Rate in Osteoclats. Connective Tissue Research 2006; 47: 222-228.
  • Palacıos C. The Role of Nutrients in Bone Health, from A to Z. Crit Rev Food Sci Nutr 2006; 46:621–628.
  • Ciombor DM. The role of electrical stimulation in bone repair. Foot Ankle Clin 2005;10: 579-593.
  • Wu H. Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells. J Huazhong Univ Sci Technolog Med Sci; 25, 2005; 2:185-187.
  • Icaro CA. Stimulation of osteoblast growth by an electromagnetic field in a model of bone-like construct. Eur J Histochem 2006; 50: 199-204.
  • Erdal N. Cytogenetic effects of extremely low frequency magnetic field on Wistar rat bone marrow. Mutat Res 2007; 630: 69-77.
  • Goodman R, Blank M. Insights into electromagnetic interaction mechanisms. J Cell Physiol 2002; 192:16-22.
  • Marmi V, Lisi A, Rieti S, et al. Low electromagnetic field (50 Hz) induces differentiation on (HOK).Bioelectromagnetics 2004; 25: 118-126. oral keratinocytes
  • Wei M, Guizzetti M, Yost M, Costa LG. Exposure to 60-Hz magnetic fields and proliferation of human astrocytoma cells in vitro. Toxicol Appi Pharmacol 2000;162 :166-176.
  • Aldinucci C, Palmi M, Sgaragli G, et al. The effect of pulsed electromagnetic fields on the physiologic behaviour of a human astrocytoma cell line. Biochim Biophys Acta 2000;1499: 101-108.
  • Manni V, Lissi A, Pozzi D, et al. Effects of extremely low frequency (50 Hz) magnetic field on morphological and biochemical properties of human keratinocytes. Bioelectromagnetics 2002;23: 298-305.
  • Pessina GP, Aldinucci C, Palmi M, et al. Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells. Bioelectromagnetics 2001;22:503-510.
  • Schiller PC, D'Ippolito G, Balkan W, Roos BA, Howard GA. Gap-junctional communication is required for the maturation process of osteoblastic cells in culture. Bone, 2001;28:362-369.
  • Zeng QL, Chiang H, Hu GL, et al. ELF magnetic fields induce internalization of gap junction protein connexin 43 in Chinese hamster lung cells. Bioelectromagnetics 2003; 24: 134-138.
  • Aaron RK, Boyan BD, Ciombor DM. Stimulation of growth factor synthesis by electric and electromagnetic fields. Clin Orthop Relat Res 2004; 419:30-37.

The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density

Yıl 2008, Cilt: 35 Sayı: 4, 240 - 244, 01.12.2008

Öz

Bu çalısmada günlük yasamda maruz kalınan oldukça düsük frekanslı (ELF) manyetik alanın (MA) kemik dokusu üzerine etkilerini arastırmayı amaçladık. Çalısmamızda 64 adet erkek Wistar-Albino sıçan kullanıldı. Rastgele sekizerli esit gruplara bölünen sıçan\'lara 45 gün süreyle uygulama yapıldı.1. gruba 1,5 mT siddetindeki ve 50 Hz frekanslı MA 4 saat/gün uygulandı. 2., 3. ve 4. grup sıçan\'lara ise sırasıyla 3.75 mg/kg, 15 mg/kg ve 60 mg/kg mangan (Mn) ile birlikte 1. gruba verilen ile aynı düzeyde MA verildi. 5. gruba (3.75 mg/kg/gün), 6. gruba (15 mg/kg/gün) ve 7. gruba (60 mg/kg/gün) dozda Mn verildi. Sekizinci grup sıçan kafes kontrol olarak kullanıldı. Tüm grupların toplam kemik mineral içerigi (TKM_)ve toplam kemik mineral yogunlugu (TKMY) degerleri ölçüldü. TKM_ yönünden 8. grup ile diger gruplar karsılastırıldıgında; 3., 4. ve 7. grup ile anlamlı degisiklik oldugu saptandı (p

Kaynakça

  • Fadel MA. Growth assessment of children exposed to low frequency electromagnetic fields at the Abu Sultan area in Ismailia (Egypt). Anthropol Anz 2006; 64: 211-226.
  • Demsia G. Effect of 910-MHz electromagnetic field on rat bone marrow. Sci World J 2004; 4 Suppl 2: 48-54.
  • Rollwitz J. Fifty-hertz magnetic fields induce free radical formation in mouse bone marrow-derived promonocytes and macrophages. Biochim Biophys Acta 2004;1674: 231-238.
  • Sievert U. Effects of electromagnetic fields emitted by cellular phone on auditory and vestibular labyrinth. Laryngorhinootologie 2007; 86: 264-270.
  • Funk RH. Effects of electromagnetic fields on cells: physiological and therapeutical approaches and molecular mechanisms of interaction. A review. Cells Tissues Organs 2006; 182: 59-78.
  • Lirani AP. Evidences of physical agents action on bone metabolism and their potential clinical use. Arq Bras Endocrinol Metabol 2005; 49: 891-896.
  • Seaman RL. Comments on "Evaluation of interactions of electric fields due to electrostatic discharge with human tissue". IEEE Trans Biomed Eng 2005; 53: 1220.
  • Binderman I, Somjen D, Shimshoni Z, et al. Stimulation of skeletal-derived cell cultures by different electric field intensities is cell-specific. Biochim Biophys Acta 1985; 844:273-279.
  • Laughlan KA, Steiner UE The spin correlated radical pair as a reaction intermediate. Mol Phys 1991; 73:241
  • Aday WR, Lawrence AF. Nonlinear Electrodynamics in Biological Systems. Plenum, New York 1984, 3–22
  • Giugni TD, Braslan DL, Haigler HT. Electric field induced redistribution and postfield relaxation of epidermal growth factor receptors on A 431 cells. J Cell Biol 1987; 104:1291–1297
  • Chang K, Chang WH, Yu YH, Shih C. Pulsed electromagnetic field stimulation of bone marrow cells derived from ovariectomized rats affects osteoclast formation and local factor production. Bioelectromagnetics 2004; 25: 1 3441.
  • Rubin J, McLeod KJ, Titus L, et al. Formation of osteoclast-like cells is suppressed by low frequency, low intensity electric fields. J Orthopaed Res 1996;14:7-15.
  • Shankar VS, Simon BJ, Bax CM, et al. Effects of electromagnetic stimulation on the functional responsiveness of isolated rat osteoclasts. J Cell Physiol 1998;176:53744.
  • Chang K, Chang WH, Tsai MT, Shih C. Pulsed Electromagnetic Fields Accelerate Apoptotic Rate in Osteoclats. Connective Tissue Research 2006; 47: 222-228.
  • Palacıos C. The Role of Nutrients in Bone Health, from A to Z. Crit Rev Food Sci Nutr 2006; 46:621–628.
  • Ciombor DM. The role of electrical stimulation in bone repair. Foot Ankle Clin 2005;10: 579-593.
  • Wu H. Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells. J Huazhong Univ Sci Technolog Med Sci; 25, 2005; 2:185-187.
  • Icaro CA. Stimulation of osteoblast growth by an electromagnetic field in a model of bone-like construct. Eur J Histochem 2006; 50: 199-204.
  • Erdal N. Cytogenetic effects of extremely low frequency magnetic field on Wistar rat bone marrow. Mutat Res 2007; 630: 69-77.
  • Goodman R, Blank M. Insights into electromagnetic interaction mechanisms. J Cell Physiol 2002; 192:16-22.
  • Marmi V, Lisi A, Rieti S, et al. Low electromagnetic field (50 Hz) induces differentiation on (HOK).Bioelectromagnetics 2004; 25: 118-126. oral keratinocytes
  • Wei M, Guizzetti M, Yost M, Costa LG. Exposure to 60-Hz magnetic fields and proliferation of human astrocytoma cells in vitro. Toxicol Appi Pharmacol 2000;162 :166-176.
  • Aldinucci C, Palmi M, Sgaragli G, et al. The effect of pulsed electromagnetic fields on the physiologic behaviour of a human astrocytoma cell line. Biochim Biophys Acta 2000;1499: 101-108.
  • Manni V, Lissi A, Pozzi D, et al. Effects of extremely low frequency (50 Hz) magnetic field on morphological and biochemical properties of human keratinocytes. Bioelectromagnetics 2002;23: 298-305.
  • Pessina GP, Aldinucci C, Palmi M, et al. Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells. Bioelectromagnetics 2001;22:503-510.
  • Schiller PC, D'Ippolito G, Balkan W, Roos BA, Howard GA. Gap-junctional communication is required for the maturation process of osteoblastic cells in culture. Bone, 2001;28:362-369.
  • Zeng QL, Chiang H, Hu GL, et al. ELF magnetic fields induce internalization of gap junction protein connexin 43 in Chinese hamster lung cells. Bioelectromagnetics 2003; 24: 134-138.
  • Aaron RK, Boyan BD, Ciombor DM. Stimulation of growth factor synthesis by electric and electromagnetic fields. Clin Orthop Relat Res 2004; 419:30-37.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Yazıları
Yazarlar

Veysi Akpolat Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2008
Gönderilme Tarihi 2 Mart 2015
Yayımlandığı Sayı Yıl 2008 Cilt: 35 Sayı: 4

Kaynak Göster

APA Akpolat, V. (2008). The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density. Dicle Tıp Dergisi, 35(4), 240-244.
AMA Akpolat V. The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density. diclemedj. Aralık 2008;35(4):240-244.
Chicago Akpolat, Veysi. “The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density”. Dicle Tıp Dergisi 35, sy. 4 (Aralık 2008): 240-44.
EndNote Akpolat V (01 Aralık 2008) The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density. Dicle Tıp Dergisi 35 4 240–244.
IEEE V. Akpolat, “The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density”, diclemedj, c. 35, sy. 4, ss. 240–244, 2008.
ISNAD Akpolat, Veysi. “The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density”. Dicle Tıp Dergisi 35/4 (Aralık 2008), 240-244.
JAMA Akpolat V. The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density. diclemedj. 2008;35:240–244.
MLA Akpolat, Veysi. “The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density”. Dicle Tıp Dergisi, c. 35, sy. 4, 2008, ss. 240-4.
Vancouver Akpolat V. The Effect of Extremely Low Frequency Magnetic Field and Manganese on Bone Mineral Content and Density. diclemedj. 2008;35(4):240-4.