Histopathological Effects of Environmental Pollutants on the Reproductive System of Zebrafish

Abstract

Environmental pollutants cause health problems as well as affect living or non-living things in terrestrial and aquatic ecosystems. Many harmful substances, the use of which is increasing today, directly or indirectly cause deterioration in the quality of life of living things. Pollutions originating from water-primarily affect fish health. Negative effects are observed on the physiological, biochemical, and anatomical structures of fish feeding, migrating, and breeding in the aquatic environment due to pollutants. Zebrafish is an important model organism thanks to its development and easy reproduction. Many potential pollutants in the aquatic environment degrade the quality of water. Natural, industrial, domestic, etc. To the aquatic environment. Pollutants of organic or inorganic origin emitted from sources can cause toxic effects on fish. Pesticides, which are considered as one of these pollutants, are used to destroy the effects of harmful pests while causing death in living things, their negative effects on the reproductive system like many systems have been revealed. Heavy metals negatively affect the biological systems of fish by causing bioaccumulation by ingestion of fish mostly through respiration. Although endocrine-disrupting chemicals (EBC) affect different tissues and organs for generations, their effects on the reproductive system increase by affecting the hormonal system. Nanoparticles affect different biological systems as well as the reproductive system. In this article, the histological effects of environmental pollutants on zebrafish ( Danio reiro) reproductive organs and the reasons for choosing zebrafish as a model organism re-evaluated.

Keywords

• Contamination
• Danio rerio
• gonad
• histopathology
• toxicity

Çevresel Kirleticilerin Zebra Balığı Üreme Sistemi Üzerindeki Etkileri

Abstract

Çevresel kirleticiler, karasal ve sucul ekosistemlerdeki canlı ya da cansız varlıklara etki etmekle beraber sağlık sorunlarına da yol açmaktadır. Günümüzde kullanımı artan pek çok zararlı içerikli madde doğrudan ya da dolaylı olarak canlıların yaşam kalitesinde bozulmalara neden olmaktadır. Su kaynaklı meydana gelen kirlilikler öncelikle balık sağlığını etkilemektedir. Akuatik çevrede beslenen, göç eden, üreyen balıkların kirleticiler nedeni ile fizyolojik, biyokimyasal, anatomik yapılarında olumsuz etkiler gözlenmektedir. Su ortamında suyun kalitesini düşüren pek çok potansiyel kirletici bulunmaktadır. Su ortamına doğal, endüstriyel, evsel vb. kaynaklardan atılan organik veya inorganik kökenli kirleticiler balıklar için toksik etkiye sebep olabilir. Bu kirleticilerden biri sayılan pestisitler, zararlı pestlerin etkilerini yok etmede kullanılırken canlılarda ölüme yol açmakla beraber pek çok sistem gibi üreme sistemindeki olumsuz etkileri ortaya konmuştur. Ağır metaller, balıkların en çok solunum yoluyla vücuda alınmasıyla biyolojik birikimlere yol açarak biyolojik sistemlerini olumsuz etkilemektedir. Endokrin bozucu kimyasallar (EBK), farklı doku ve organlarda etki göstererek nesiller boyu etkilenmeyi sağlamakla beraber hormonal sistemi etkilemesiyle üreme sistemi üzerindeki etkileri artmaktadır. Nanopartiküller, üreme sistemi ile birlikte farklı biyolojik sistemlere de etki etmektedir. Bu derleme makalede çevresel kirleticilerin zebra balığı (Danio rerio) üreme sistemi üzerindeki histolojik etkilerinin değerlendirilmesi ile beraber zebra balığının model organizma olarak seçilmesinin nedenleri anlatılmaktadır.

Keywords

• Zebra balığı
• toksisite
• kirlilik

References

1. Referans1 M. Ahamed, M. S. Alsalhi, and M. K. J. Siddiqui, “Silver nanoparticle applications and human health,” Clinica Chimica Acta, vol. 41, pp. 1841–1848, 2010.
2. Referans2 C. Akbulut, and N.D. Yon, “Histopathology And Cellular Apoptosis At Spermatogenic Cells Of Zebrafish (Danio rerio) At Acute And Sub-Chronic Exposure Of Titanium Dioxide (Tio2) Nanoparticles,” Fresenius Environmental Bulletin, vol. 8, pp. 2991-2997, 2016.
3. Referans3 C. Akbulut, and N.D. Yon, “Bisfenol a’nın zebra balıklarında (danio rerio) teratolojik etkileri,” Sakarya Üniversitesi Fen Bilimleri DErgisi c. 17, s.1, ss. 105-111, 2013.
4. Referans4 Y. Aktop, and İ. T. Cagatay, “Ağır Metallerin Balıklarda Birikimi Ve Etkileri,” Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, c. 6, ss. 37-44, 2020.
5. Referans5 S. Arman, “Pestisit Kaynaklı Kardiyak Toksisite Araştırmalarında Zebra Balığı (Danio rerio),” Düzce Üniversitesi Bilim ve Teknoloji Dergisi, c. 7, ss. 1417-1430, 2019.
6. Referans6 M. Abar Gürol, S. Arman, and N.D. Yon, “Effects Of Mancozeb On The Testicular Histology Of The Zebrafish (Danio rerio),” International Journal of Limnology, vol. 56, pp. 10, 2020.
7. Referans7 C. Bai and M. Tang “Toxicological study of metal and metal oxide nanoparticles in zebrafish,” Journal of Wiley Applied Toxicology, vol.40, pp. 37–63, 2020.
8. Referans8 M. N. H. Bhujyan, H. Kang, J Choi, S. Lim, Y. Kho, and K. Choi, “Effects of 3,4-dichloroaniline (3,4-DCA) and 4,40-methylenedianiline (4,40-MDA) on sex hormone regulation and reproduction of adults,” Chemosphere, vol. 269, pp. 128768, 2021.

9. Referans9 L. Braydich-Stolle, S. Hussain, J. J. Schlager and M. C. Hofmann, “In Vitro Cytotoxicity of Nanoparticles in Mammalian Germline Stem Cells. Toxicological Sciences,” vol. 88, pp. 412–419, 2005.
10. Referans10 J. Cao, G. Wang, T. Wang, G. Chen Wenjing, P Wu, X. He and L. Xie, “Copper caused reproductive endocrine disruption in zebrafish (Danio rerio),” Aquatic Toxicology, vol. 211, pp. 124–136, 2019.
11. Referans11 C. Chakraborty, A. R. Sharma, G. Sharma and S. S. Lee, “Zebrafish: A complete animal model to enumerate the nanoparticle toxicity,” Journal Nanobiotechnology, vol. 14, pp. 65, 2016.
12. Referans12 S. X. Chen, X. Z. Yang Y. Deng, J. Huang, Y. Li, Q. Sun, C. P. Yu, Y. Zhu and W. S. Hong, “Silver nanoparticles induce oocyte maturation in zebrafish (Danio rerio),” Chemosphere vol. 170 pp. 51-60, 2017.
13. Referans13 H. Chen, K. Chen, X. Qiu, H. Xu, G. Mao, T. Zhao, W. Feng, E. S. Okeke, X. Wu and L. Yang, “The reproductive toxicity and potential mechanisms of combined exposure to dibutyl phthalate and diisobutyl phthalate in male zebrafish (Danio rerio),” Chemosphere, vol. 258 pp. 127238, 2020.
14. Referans14 H. Cheng, W. Yan, Q. Wu, J. Lu, C. Liu, T. C. Hung and G. Li, “Adverse reproductive performance in zebrafish with increased bioconcentration of microcystin-LR in the presence of titanium dioxide nanoparticles,” Environmental Science Nano, vol. 5, pp. 1208–1217, 2018.
15. Referans15 S. Çetinkaya, “Endokrin çevre bozucular ve ergenlik üzerine etkileri,” Dicle Tıp Dergisi, c. 36, ss. 59-66, 2009.
16. Referans16 M. Darvishi, R. Safari, S. H. Hoseinifar, A. Shabani, M. Dadar, Z. Jarayedi and M. Paolucci, “Sublethal doses of diazinon affected reproductive, immune, and oxidative status in female zebrafish (Danio rerio),” Aquaculture Reports, vol. 22, pp. 100944, 2022.
17. Referans17 C. E. Davico, A. G. Pereira, L. Nezzi, M. L. Jaramillo, M. Silveira de Melo, Y. M. R. Müller and E. M. Nazari, “Reproductive toxicity of Roundup WG® herbicide: impairments in ovarian follicles of model organism Danio rerio. Environmental Science and Pollution Research,” vol. 28, pp. 15147–15159, 2021.
18. Referans18 N. Dayal, Thakur M., P. Patil, D. Singh, G. Vanage and D. Joshi, “Histological and genotoxic evaluation of gold nanoparticles in ovarian cells of zebrafish (Danio rerio),” Journal of Nanoparticle Research, vol. 18, pp. 291, 2016.
19. Referans19 K. Donaldson, V. Stone, L. Tran, W. G. Kreyling and P. Borm, “Nanotoxicology,” Occupational and Environmental Medicine, vol. 61, pp. 727–8, 2004.
20. Referans20 N. Lewinski, V. Colvin, and R. Drezek, R. “Cytotoxicity Of Nanoparticles,” Small-Journal, vol. 4, pp. 26 – 49, 2008.
21. Referans21 L. A. Dykman and N. G. Khlebtsov, “Gold nanoparticles in biology and medicine: recent advances and prospects," Acta Naturae, vol. 3, pp. 34–55, 2011.
22. Referans22 E. Esmeray, and O. Ozata, “Nanopartiküllerin Çevre Mühendisliğinde Kullanımı ve Temel Laboratuvar Malzemeleri ile Gümüş Nanopartikül (AgNPs) Sentezi,” Avrupa Bilim Ve Teknoloji Dergisi, c. 16, ss. 521-527, 2019.
23. Referans23 . Fang, Q. Shi, Y. Guo, J. Hua, X. Wang and B. Zhou, “Enhanced Bioconcentration of Bisphenol A in the Presence of NanoTiO2 Can Lead to Adverse Reproductive Outcomes in Zebrafish,” Environmental Science & Technology, vol. 19;50, pp. 1005-13, 2015.
24. Referans24 I. Forner-Piquer, S. Beato, F. Piscitelli, S. Santangeli, V. Di Marzo, H. R. Habibi,F. Maradonna and O. Carnevali, “Effects Of Bisphenol-A On Zebrafish Gonads: Focus On The Endocannabinoid System,” Environmental Pollution, vol. 264, pp. 114710, 2020.
25. Referans25 N. Hassanzadeh, “Histopathological Evaluation Of The Zebrafish (Danio rerio) Testis Following Exposure To Methyl Paraben,” International Journal Of Aquatic Biology Dergisi, vol. 5, pp. 71-78, 2007.
26. Referans26 T. C. K. Heiden, C. A. Struble, M. L. Rise, M. J. Hessner, R. J. Hutz and M. J. Carvan, “Molecular targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the zebrafish ovary: Insights into TCDD-induced endocrine disruption and reproductive toxicity,” Reproductive Toxicology, vol. 25, pp. 47–57, 2008.
27. Referans27 J. Hou, L. Li, N. Wu, Y. Su, W. Lin, G. Li and Z. Gu, “Reproduction impairment and endocrine disruption in female zebrafish after long-term exposure to MC-LR: A life cycle assessment,” Environmental Pollution, vol. 208, pp. 477-485, 2016.
28. Referans28 L. P. Hou, Y. Yang, H. Shu, G. G. Ying, J. L. Zhao, Y. B. Chen, Y. H. Chen, G. Z. Fang, X. Li, and J. S. Liu, “Changes in Histopathology, Enzyme Activities, and the Expression of Relevant Genes in Zebrafish (Danio rerio) Following LongTerm Exposure to Environmental Levels of Thallium” Bulletin of Environmental Contamination and Toxicology, vol. 99, pp. 574–581, 2017.
29. Referans29 L. Hou, S. Chen S, W. Shi, H. Chen, Y. Liang, X. Wang, J. Tan, Y. Wang, X. Deng, M. Zhan, J. Long, G. Cai, S Luo, C. Zhang, J Liu, J. Y. S Leung and L Xie, “Norethindrone Alters Mating Behaviors, Ovary Histology, Hormone Production And Transcriptional Expression Of Steroidogenic Genes İn Zebrafish (Danio rerio),” Ecotoxicology And Environmental Safety, vol. 195, pp. 110496, 2020.
30. Referans30 E. Houdeau, L. Bruno, L. Dominique and P. Fabrice, “Nanoparticles and food: an emerging risk in human health?,” Cahiers de Nutrition et de Diététique, vol. 433, pp. 10, 2018.
31. Referans31 X. Huang, I. H. El-Sayed, W. Qian and M. A. Al-Sayed, “Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods,” Journal of the American Chemical Society, vol. 128, pp. 2115–20, 2006.
32. Referans32 M. Jia, M. Teng, S. Tian, J. Yan, Z. Meng, S. Yan, R. Li, Z. Zhou and W. Zhu, “Effects of penconazole enantiomers exposure on hormonal disruption in zebrafish Danio rerio (Hamilton, 1822,” Environmental Science and Pollution Research, vol. 28, pp. 43476–43482, 2021.
33. Referans33 J. Jiang, L. Chen, X. Liu, L. Wang, S. Wu and X. Zhao, “Histology and multi-omic profiling reveal the mixture toxicity of tebuconazole and difenoconazole in adult zebrafish,” Science of the Total Environment, vol. 795, pp. 148777, 2021.
34. Referans34 S. Jin and K. Ye, “Nanoparticle-mediated drug delivery and gene therapy,” Biotechnology Progress, vol. 23, pp. 32–41, 2007.
35. Referans35 S. Barillet, V. Larno, M. Floriani, A. Devaux, C. Adam-Guillermin, “Ultrastructural effects on gill, muscle, and gonadal tissues induced in zebrafish (Danio rerio) by a waterbone uranium exposure,” Aquatic Toxicology, vol. 100, pp. 295-302, 2010.
36. Referans36 B. Kaptaner and G. Ünal, “17α-Etinilestradiol ve 4-n-Nonilfenol’e Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler,” Doğu Fen Bilimleri Dergisi, c. 3, s.2, ss. 83-94, 2020.
37. Referans37 F. E. Kayhan, G. Kaymak, H. E. Esmer Duruel and S. Tartar Kızılkaya, “Biyolojik Araştırmalarda Zebra Balığının (Danio rerio Hamilton, 1822) Kullanılması ve Önemi,” Journal of Gaziosmanpasa Scientific Research, c. 7, s. 2, ss. 38-45, 2018.
38. Referans38 L. Kernen, A. Phan, J. Bo, E. L. Herzog, J. Huynh, H. Segner and L. Baumann, “Estrogens as immunotoxicants: 17α-ethinylestradiol exposure retards thymus development in zebrafish (Danio rerio),” Aquatic Toxicology vol. 242, pp. 106025, 2022.
39. Referans39 S. Klein, S. Petersen, U. Taylor, D. Rath and S. Barcikowski, “Quantitative visualization of colloidal and intracellular gold nanoparticles by confocal microscopy,” Journal of Biomedical Optics, vol. 15, pp. 036015, 2010.
40. Referans40 N. D. Koc, M. N. Muslu, F. E. Kayhan and S. Ozesen Colak, “Histopathological Changes In Ovaries Of Zebrafish (Danio rerio) Following Administration Of Deltamethrin” Fresenius Environmental Bulletin, vol. 18(10), pp. 1872-1878, 2009.
41. Referans41 T. Kotil, C. Akbulut and N. D. Yon, “The Effects Of Titanium Dioxide Nanoparticles On Ultrastructure Of Zebrafish Testis (Danio rerio),” Micron, vol. 100, pp. 38-44, 2017.
42. Referans42 E. Kökdemir Ünşar, and N. A. Perendeci, “Nanopartiküllerin çevresel akıbetleri ve anaerobik parçalanma prosesine etkileri,” Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, c. 22(6), ss. 503-512, 2015.
43. Referans43 X. R. Lan, Y. W. Li, Q. L. Chen, Y. J. Shen and Z. H. Liu, “Tributyltin impaired spermatogenesis and reproductive behavior in male zebrafish,” Aquatic Toxicology, vol. 224, pp. 105503, 2020.
44. Referans44 M. Li, J. Cao, J. Chen, J. Song, B. Zhou, C. Feng and J. Wang, “Waterborne fluoride exposure changed the structure and the expressions of steroidogenic-related genes in gonads of adult zebrafish (Danio rerio),” Chemosphere, vol. 145 pp. 365-375, 2016.
45. Referans45 S Li, Q. Sun, Q. Wu, W. Gui, G. Zhu and D. Schlenk, “Endocrine Disrupting Effects of Tebuconazole on Different Life Stages of Zebrafish (Danio rerio),” Environmental Pollution, vol. 249, pp. 1049-1059, 2019.
46. Referans46 Y. F. Li and C. Chen, “Fate and toxicity of metallic and metal-containing nanoparticles for biomedical applications,” Small, vol. 7(21), pp. 2965–2980, 2011.
47. Referans47 X. L. Liao, Z. F. Chen, T. Zou, Z. C. Lin, X. F. Chen, Y. Wang, Z. Qi and Z. Cai, “Chronic Exposure to Climbazole Induces Oxidative Stress and Sex Hormone Imbalance in the Testes of Male Zebrafish,” Environmental Research, vol. 184, pp. 109310, 2020.
48. Referans48 Z. Z. J. Lim, J. E. J. Li, C. T. Ng, L. Y. L. Yung and B. H. Bay, “Gold nanoparticles in cancer therapy,” Acta Pharmacologica Sinica, vol. 32, pp. 983–90, 2011.
49. Referans49 P. Liu, Y. Zhao, S. Wang, H. Xing and W. F. Dong, “Effect of combined exposure to silica nanoparticles and cadmium chloride on female zebrafish ovaries,” Environmental Toxicology and Pharmacology, vol. 87, pp. 103720, 2021.
50. Referans50 A. J. Lora, A. M. Molina, C. Bellido, A. Blanco, J. G. Monterde and M. R. Moyano, “Adverse Effects Of Bisphenol A On The Testicular Parenchyma Of Zebrafish Revealed Using Histomorphological Methods,” Veterinarni Medicina, vol. 61(10), pp. 577–589, 2016.
51. Referans51 J. Lu, Q. Wu, Q. Yang, G. Li, R. Wang, Y. Liu, C. Duan, S. Duan, X. He, Z. Huang, X. Peng, W. Yan and J. Jiang, “Molecular mechanism of reproductive toxicity induced by beta-cypermethrin in zebrafish,” Comparative Biochemistry and Physiology, Part C vol. 239, pp. 108894, 2021.
52. Referans52 A. Luzio, S. M. Monterio, E. Rocha, A. A. Fontainhas-Fernandes and A. M. Coimbra, “Development And Recovery Of Histopathological Alterations İn Thegonads Of Zebrafish (Danio rerio) After Single And Combined Exposure to Endocrine Disruptors (17-Ethinylestradiol And Fadrozole),” Aquatic Toxicology, vol. 175, pp. 90–105, 2016.
53. Referans53 Y. B. Ma, C. J. Lu, M. Junaid, P. P. Jia, L. Yang, J. H. Zhang and D. S. Pei, “Potential adverse outcome pathway (AOP) of silver nanoparticles mediated reproductive toxicity in zebrafish. Elsevier dergisi,” Chemosphere, vol. 207, pp. 320-328, 2018.
54. Referans54 K. Maharajan, S. Muthulakshmi, C. Karthik, B. Nataraj, K. Nambirajan, D. Hemalatha, S. Jiji, K. Kadirvelu, K. C. Liu and M. Ramesh, “Pyriproxyfen İnduced İmpairment Of Reproductive Endocrine Homeostasis And Gonadal Histopathology İn Zebrafish (Danio rerio) By Altered Expression Of Hypothalamus-Pituitary-Gonadal (Hpg) Axis Genes. Science Of The Total Environment, vol. 735, pp. 139496, 2020.
55. Referans55 B. Manjunatha and G. H. Philip, “Reproductive Toxicity Of Chlorpyrifos Tested İn Zebrafish (Danio rerio): Histological And Hormonal End Points. Toxicology And Industrial Health,” Sage Journals, vol. 32(10), 1808-1816, 2015.
56. Referans56 L. Molina, A. M. Abril, N. L. Benitez, A. Huertas-Abril, P. V. A. Soldado, N. Blanco, C. M. Salvago and M. Rosario, “Proteomic profile of the effects of low-dose bisphenol A on zebrafish ovaries,” Food and Chemical Toxicology, vol. 156, pp. 112435, 2021.
57. Referans57 X. Mu, S. Qi, J. Liu, H. Wang, L. Yuan, L. Qian, T. Li, Y. Huang, C. Wang, Y. Guo and Y. Li, “Environmental level of bisphenol F induced reproductive toxicity toward zebrafish,” Science of the Total Environment vol. 806, pp. 149992, 2022.
58. Referans58 M. T. Noman, M. A. Ashraf and A. Ali, “Synthesis and applications of nano‐TiO2: A review,” Environmental Science and Pollution Research, vol. 26, pp. 3262–3291, 2018.
59. Referans59 A. Orbea, N. Gonzalez-Soto, J. M. Lacave, I. Barrio and M. P. Cajaraville, “Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish,” Comparative Biochemistry and Physiology, Part C vol. 199, pp.59–68, 2017.
60. Referans60 K. Ou-Yang, T. Feng, Y. Han, G. Li and H. Ma, “Bioaccumulation, metabolism, and endocrine-reproductive effects of metolachlor and its S-enantiomer in adult zebrafish (Danio rerio),” Science of the Total Environment vol. 802, pp. 149826, 2022.
61. Referans61 G. Ozbolat, and A. Tuli, “Effects of Heavy Metal Toxicity on Human Health,” Archive Source Review Journal, vol. 25(4), pp. 502-521, 2016.
62. Referans62 P. Perugini, S. Simeoni, S. Scalia, I. Genta, T. Modena, B. Conti and F. Pavanetto, “Effect of nanoparticle encapsulation on the photostability of the sunscreen agent, 2-Ethylhexyl-p-methoxycinnamate,” International Journal of Pharmaceutics, vol. 246, pp. 37–45, 2002.
63. Referans63 A. Petrovici, S. A. Strungaru, M. Nicoara., M. A. Robea, C. Solcan and C. Faggio, “Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers,” Journal of Marine Science and Engineering, vol. 8, pp. 73, 2020.
64. Referans64 G. K. Pitchika, K. V. Swamy, T. S. Ranjani, C. R. Tyler, S. B. Sainath and G. H. Phillip, “Effect of cypermethrin on Reproductive efficacy in zebrafish (Danio rerio): In vivo and silico studies,” Journal of Environmental Biology, vol. 40, pp. 985-994, 2019.
65. Referans65 T. Prow, R. Grebe, C. Merges, J. Smith, D. S. Mcleod, J. F. Leary and G. Lutty, “Nanoparticle tethered biosensors for autoregulated gene therapy in hyperoxic endothelium,” Nanomedicine: Nanotechnology, Biology and Medicine, vol. 2, pp. 276, 2006.
66. Referans66 J. Y. Qin, S. Ru, W. Wang, L. Hao, S. Wei, J. Zhang, J. Q. Xiong, J. Wang and X. Zhang, “Unraveling the mechanism of long-term bisphenol S exposure disrupted ovarian lipids metabolism, oocytes maturation, and offspring development of zebrafish,” Chemosphere vol. 277, pp. 130304, 2021.
67. Referans67 L. Rocco, M. Santonastaso, F. Mottalo, D. Costagliola, T. Suero, S. Pacifico and V. Stingo, “Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio,” Ecotoxicology and Environmental Safety, vol. 113, pp. 223–230, 2015.
68. Referans68 N. Sataloğlu, B. Aydın and A. Turla, “Pestisit Zehirlenmeleri,” TSK Koruyucu Hekimlik Bülteni, c. 6 (3), ss. 169-174, 2007.
69. Referans69 T. Seven, B. Can, B. N. Darende and S. Ocak, “Hava ve Toprakta Ağır Metal Kirliliği,” c.1(2), ss. 91-103, 2018.
70. Referans70 W. J. Shi, D. D. Ma, Y. X. Jiang, L. Xie, J. N. Zhang, G. Y. Huang, H. X. Chen, L. P. Hou, Y. S. Liu and G. G. Ying, “Medroxyprogesterone Acetate Affects Sex Differentiation And Spermatogenesis İn Zebrafish,” Aquatic Toxicology, vol. 212, pp. 70–76, 2019.
71. Referans71 D. Simon, S. Helliwell and K. Robards, “Analytical chemistry of chlorpyrifos and diuron in aquatic ecosystems,” Analytica Chimica Acta, vol. 360, pp. 1-16, 1998.
72. Referans72 O. Simon, B. Gagnaire, V. Camilleri, I. Cavalie, M. Floriani and C. Adam-Guillermin, “Toxicokinetic and toxicodynamic of depleted uranium in the zebrafish, Danio rerio,” Aquatic Toxicology, vol. 197, pp. 9–18, 2018.
73. Referans73 G. Singhal, R. Bhavesh, A. R. Sharma and R. P. Singh, “Ecofriendly biosynthesis of gold nanoparticles using medicinally important Ocimum basilicum leaf extract,” Advanced Science, Engineering and Medicine, vol. 4, pp. 62–66, 2012.
74. Referans74 V. M. Şimşek and N. Uygun, “Bazı tarımsal savaş ilaçlarının turunçgil bahçelerindeki önemli parazitoit ve predatörlere etkilerinin laboratuvar koşullarında araştırılması,” Türkiye biyolojik mücadele dergisi, c. 4 (2), ss. 141-154, 2013.
75. Referans75 B. Tang, J. Tao, S. Xu, J. Wang, C. Hurren, W. Xu, L. Sun and X. Wang, “Using hydroxy carboxylate to synthesize gold nanoparticles in heating and photochemical reactions and their application in textile coloration,” Chemical Engineering Journal, vol. 172, pp. 601-607, 2011.
76. Referans76 M. Tang and C Bai, “Toxicological study of metal and metal oxide nanoparticles in zebrafish,” Journal of Applied Toxicology, vol. 40, pp. 37–63, 2019.
77. Referans77 Z. S. Taylan and H. Böke Özkoç, “Potansiyel ağır metal kirliliğinin belirlenmesinde akuatik organizmaların biokullanılabilirliliği,” BAÜ FBE Dergisi, c. 9(2), ss. 17-33, 2007.
78. Referans78 M. Teng, C. Wang, M. Song, X. Chen, J. Zhang and C. Wang, “Chronic Exposure Of Zebrafish (Danio rerio) To Flutolanil Leads To Endocrine Disruption And Reproductive Disorders,” Environmental Research, vol. 184, pp. 109310, 2020.
79. Referans79 N. Vural, “Toksikoloji,”Ankara, Türkiye: Ankara Universitesi Basimevi, 2005, böl. 3, ss. 344-401.
80. Referans80 C. Wang, J. Zhu, X. Gong, Y. Liang, S. Xu and Y. Yu, “Bioaccumulation of BDE47 in testes by TiO2 nanoparticles aggravates the reproductive impairment of male zebrafish by disrupting intercellular junctions. Nanotoxicology, vol. 15:8 pp.1073-1086, 2021.
81. Referans81 G. Wang, A. S. Stender, W. Sun and N. Fang, “Optical imaging of non-fluorescent nanoparticle probes in live cells,” Analyst, vol. 135, pp. 215–221, 2010.
82. Referans82 J. Wilson-Raw, Z. Zhao, X. Zhang, Y. Chen, H. Liu, Y. Chang, R. George and J. Wang, “Disruption of zebrafish (Danio rerio) reproduction upon chronic exposure to TiO2 nanoparticles.,” Chemosphere, vol. 83, pp. 461–467, 2010.
83. Referans83 C. Weiss and S. Diabate, “A special issue on nanotoxicology,” Archives of Toxicology, vol. 85, pp. 705–706, 2011.
84. Referans84 H. Wu, Q. Rao, J. Zheng, C. Mao, Y. Sun, D. Gu, M. Wang and X. Liu, “Biochemical And Histological Alterations İn Adult Zebrafish（Danio rerio）Ovary Following Exposure To The Tetronic Acid İnsecticide Spirotetramat,” Ecotoxicology And Environmental Safety, vol. 164, pp. 149–154, 2018.
85. Referans85 Q. Xu, D. Wu, Y. Dang, L. Yu, C. Liu and J. Wang, “Reproduction impairment and endocrine disruption in adult zebrafish (Danio rerio) after waterborne exposure to TBOEP,” Aquatic Toxicology, vol. 182, pp. 163–171, 2017.
86. Referans86 R. Yang, X. Wang, J. Wang, P. Chen, Q. Liu, W. Zhong and L. Zhu, “Insights into the sex-dependent reproductive toxicity of 2-Ethylhexyl diphenyl phosphate on zebrafish (Danio rerio),” Environment International, vol. 158, pp. 106928, 2022.
87. Referans87 Y. Yıldırım, N. Ertaş Onmaz N., Z. Gonulalan, H. Hızlısoy, S. Al, C. Candemir Güngör, H. B. Dişli, A. Dışhan and M. Barel, “Bisfenoller ve Fitalatların Halk Sağlığı Üzerine Etkileri,” c. 17(1), ss. 68-75, 2020.
88. Referans88 B. Yıldız Fendoğlu, B. Koçer-Gümüşel and P. Erkekoglu, “Endokrin Bozucu Kimyasal Maddelere ve Etki Mekanizmalarına Genel Bir Bakış,” Hacettepe University Journal Of The Faculty Of Pharmacy, c. 39(1), ss. 30-43, 2019.
89. Referans89 K. Y. Yoon, J. H. Byeon, J. H. Park and J. Hwang, “Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles,” Science of the Total Environment, vol. 373, pp. 572–575, 2007.
90. Referans90 M. Yu, Y. Feng, X. Zhang, J. Wang, H. Tian, W. Wang and S. Ru, “Semicarbazide Disturbs The Reproductive System Of Male Zebrafish (Danio rerio) Through The Gabaergic System,” Reproductive Toxicology, vol. 73, pp. 149-157, 2017.
91. Referans91 H. Segner, “Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption,” Comparative Biochemistry and Physiology, Part C vol. 149, pp. 187–195, 2009.
92. Referans92 E. V. Paravani, M. F. Simoniello, G. L. Poletta and V. H. Casco, “Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells,” Ecotoxicology and Environmental Safety, vol. 173, pp. 1–7, 2019.
93. Referans93 N. Berik, “Titanyum Dioksit ve Nano Titanyum Dioksit Kullanımının Su Ürünlerine Olası Zararları”, Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries, c.1, s.2, ss. 59-65, 2018.